Method for preparing high-purity glucomannan

A glucomannan, high-purity technology, applied in the direction of pharmaceutical formulations, medical preparations with non-active ingredients, medical preparations containing active ingredients, etc., can solve the problems affecting the purity and color of white and polysaccharides, and achieve the The effect of low content, controllable quality and high purity

Active Publication Date: 2016-06-15
SUZHOU QIUSHI HERBAL HEALTH TECH CO LTD
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0004] During the further development of the preparation process of Baiji polysaccharide, the inventor found that although the water extraction and alcohol precipitation process can effectively obtain Baiji polysaccharide, but due to the high viscosity of the extract of Baiji polysaccharide, the polyphenols coexisting in the extract during the alcohol precipitation process Impurities such as pigments and insoluble particles will be precipitated together, which will affect the purity and color of white and polysaccharides; some organic substances such as oligosaccharides will also be precipitated during the alcohol precipitation process during acid hydrolysis

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0024] Example 1: Preparation of Baiji polysaccharide BT180

[0025] Baiji medicinal material is a commercial product, and the polysaccharide contained in Baiji is analyzed by HPGPC as a homogeneous polysaccharide of Mw186kD.

[0026] (1) Weigh 4.0kg of Baiji herb decoction pieces, add 80kg and 70kg of water to extract twice, each time for 1.5hr, concentrate the extract to 40L, add 1.5 times of 95% ethanol to precipitate, and filter with 200 mesh filter cloth to obtain Baiji polysaccharide crude product GM-A (yield 15% based on solid content); take 60g of GM-A (calculated based on solid content, the same below), dissolve it in 3L water at 60°C, and prepare 2.0% GM-A solution;

[0027] (2) Add 20g of granular activated carbon filter aid to 2.0% GM-A, boil for 30min, let stand overnight at room temperature, filter with absorbent cotton and filter paper, and obtain 2.7L of light yellow solution GM-B;

[0028] (3) Filter GM-B with a 450nm water-based microporous membrane to obta...

Embodiment 2

[0032] Example 2: Preparation of Baiji polysaccharide BT10

[0033] Baiji polysaccharide 10 was prepared by acid hydrolysis, and it was a homogeneous polysaccharide of Mw16kD by HPGPC analysis.

[0034] (1) Weigh 20g of Baiji polysaccharide 10 crude product GM-A, dissolve it in 200mL water for injection at 60°C, and prepare a 10.0% GM-A solution;

[0035] (2) Add 0.2g of activated carbon filter aid for injection to GM-A, boil for 30min, let stand at room temperature for 4h, filter with absorbent cotton and filter paper, and obtain 180mL of nearly colorless solution GM-B;

[0036] (3) Use 450nm, 200nm water microporous membranes to filter GM-B successively to obtain 170mL of transparent and nearly colorless solution GM-C;

[0037] (4) Concentrate GM-C with a polysulfone ultrafiltration membrane module with a molecular weight cut-off of 2kD, and at the same time add a total of 500mL of water for injection to the ultrafiltration circulating fluid, and finally obtain a transparen...

Embodiment 3

[0040] Example 3: Preparation of Baiji polysaccharide BT180 oral liquid

[0041] (1) Weigh 60 g of the crude polysaccharide GM-A of Example 1, dissolve it in 3 L of water at 60° C., and prepare a 2.0% GM-A solution;

[0042] (2) Add 30 g of granular activated carbon filter aid to 2.0% GM-A, boil for 30 min, let stand at room temperature for 6 h, centrifuge, filter through absorbent cotton and filter paper, and obtain a light yellow solution GM-B 2.8L;

[0043] (3) Use 450nm and 220nm water microporous membranes to filter GM-B successively to obtain transparent light yellow solution GM-C 2.7L;

[0044] (4) Concentrate GM-C with a polysulfone ultrafiltration membrane module with a molecular weight cut-off of 40kD, and at the same time add a total of 6L of pure water to the ultrafiltration circulating fluid, and finally obtain a transparent and colorless retentate GM-D2.7L;

[0045] (5) The GM-D obtained in (4) was prepared by adding purified water to a final volume of 5.4L, sub...

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Abstract

The invention discloses a method for preparing high-purity glucomannan. The method comprises main steps as follows: a solution with the concentration of 0.5%-10.0% is prepared from homogeneous polysaccharide raw materials which have the molecular weight in a range of 10-1,000 kD and are selected from naturally sourced konjac polysaccharides, Bletilla striata polysaccharides or hydrolysates of the konjac polysaccharides or Bletilla striata polysaccharides; a filter aid is added to the solution for treatment, an obtained mixture is filtered and is sequentially filtered with a 100-1,000-nm micro-filtration membrane and concentrated with an ultrafiltration membrane with the molecular weight cut off of 2-100 kD, an obtained product is concentrated and dried, and the high-purity glucomannan with the purity of 90% or higher or a liquid preparation of the high-purity glucomannan is obtained. The method has the advantages that the preparation process is concise and controllable, and enlarged production is realized easily; the obtained glucomannan has high purity and low content of related substances and can meet the quality standard for medicinal bulk pharmaceutical chemicals, thereby being applicable to preparation of drugs and related medical products such as pharmaceutical excipients, medical equipment and the like.

Description

technical field [0001] The invention relates to a glucomannan extract extracted from natural plants, and also relates to the application of the glucomannan extract in the preparation of medicines and medical products, belonging to the field of biomedicine. technical background [0002] Glucomannan is a common natural viscous polysaccharide. Its main chain is composed of D-mannose and D-glucose. grooming. Such polysaccharides are mainly found in plants, such as konjac, aloe, bletilla striata (also known as bletilla striata, etc.), and are widely used in natural food, cosmetics, medical supplies and other fields, and have significant economic value. [0003] Baiji polysaccharide is the main active ingredient in traditional Chinese medicine Baiji, which has the functions of astringent, hemostasis, and nourishing the lungs, and is used in related medicines. The existing Baiji polysaccharide (also known as Baiji gum) generally adopts the production process of water extraction a...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C08B37/02A61K31/736A61K47/36
CPCA61K31/736A61K47/36C08B37/0003C08B37/0087
Inventor 刘江云蔡培烈杨世林
Owner SUZHOU QIUSHI HERBAL HEALTH TECH CO LTD
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