Molecular marker for identifying sesame male sterile line of sesame and identifying method thereof

Abstract

The invention provides a molecular marker for identifying sesame male sterile line of sesame and an identifying method thereof. The molecular marker uses Primer Premier 5.0 software to design primers (InDel70F and InDel70R); when a homozygous recessive sterile plant is amplified by the primers, the length of an amplifying fragment is 140bp; when a dominant homozygous fertile plant is amplified, the length of an amplifying fragment is 130bp; when a dominant hybrid fertile plant is amplified, two amplifying fragments are used, wherein the length of one amplifying fragment is 140bp, and the length of the other amplifying fragment is 130bp. The molecular marker has the advantages that the male sterility aided selection of the sesame is performed by the molecular marker, the transformation cycle is shortened, the transformation efficiency is improved, the troublesome procedure of selfing identification of sterility required by each generation in the transformation process is not needed, the traditional phenotype selection is converted into the gene selection, and the accuracy and scientificity of selection are improved; the male sterile line of the sesame is identified by the molecular marker in the seedling period, the production purity of sesame seeds is guaranteed, the labor is saved, the cost is reduced, and the working efficiency is improved.

Description

technical field [0001] The invention relates to the field of biotechnology, in particular to a molecular marker for identifying sesame cell nuclear male sterile lines and an identification method thereof. Background technique [0002] Sesame (Sesamumindicum L.) is an important high-quality oil crop and characteristic agricultural product in my country, and it is also an important export-earning agricultural product. In recent years, the contradiction between the supply and demand of edible oil in our country is very prominent. Taking various measures to continuously increase the output of sesame and improve the production efficiency of sesame is a major topic of sesame research. The problem in sesame production is that the yield per unit area is low and unstable. Sesame varieties are very sensitive to environmental variability, including lack of tolerance to disease, waterlogging, and response to changes in light and temperature. Practice has proved that sesame hybrids hav...

AI Technical Summary

Problems solved by technology

The problem in sesame production is that the yield per unit area is low and unstable

At present, sesame uses the male sterile two-line method for hybrid seed production. The two-line seed production technology used in production is not perfect, and only 50% of the production population is sterile. During the seed production process, it is necessary to remove the anthers according to the characteristics of the anthers during the flowering stage. Fertile plants in female parent row

This is time-consuming and laborious, increases the cost of seed production, and the purity of the seeds cannot be guaranteed, which undoubtedly limits the application of sesame hybrids to a certain extent, so the early identification of sesame male sterile lines is particularly important.

Images