Carbofuran bio-barcode immunoassay kit and application thereof

A biological barcode and immunoassay technology, which is applied in the field of Carboweil biological barcode immunoassay assay kits, can solve problems such as inability to apply Carboweil, and achieve the effects of ensuring sensitivity, facilitating operation and production, and improving sensitivity

Active Publication Date: 2016-06-22
INST OF QUALITY STANDARD & TESTING TECH FOR AGRO PROD OF CAAS
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

The kit is detected through a competitive reaction system, which overcomes the defect that the "sandwich" structure mode adopted by the current biological barcode immunoassay method is basically not suitable for the detection of carbofuran

Method used

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  • Carbofuran bio-barcode immunoassay kit and application thereof
  • Carbofuran bio-barcode immunoassay kit and application thereof
  • Carbofuran bio-barcode immunoassay kit and application thereof

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0051] Preparation method of carbofuran biological barcode immunoassay assay kit

[0052] S11. Preparation of single-label colloidal gold probe

[0053] 1), gold nanoparticle synthesis method

[0054] Add 98mL of deionized water to the siliconized beaker respectively, and then add 2mL of 50mM chloroauric acid stock solution respectively, so that the concentration of chloroauric acid is 1mM, stir on a magnetic stirrer at 1000rpm / min, heat at 250°C, and continue to boil after the liquid boils 2min; absorb 10mL of 38.8mM trisodium citrate, quickly add it to the beaker at one time, keep the stirring speed and heating temperature constant, and continue to boil for 6min. It can be found that the color of the solution gradually changes from colorless to purple, and then into wine red. The different reducing doses of trisodium citrate are added to eventually turn into different colors. After the liquid is cooled, deionized water is used to restore the original volume, and it is store...

Embodiment 2

[0082] Kit use method of the present invention

[0083] The specific operations of the Carbofuran biological barcode immunoassay assay kit prepared in the above implementation case 1 are as follows:

[0084] S21. Take out the kit from the refrigerator at 4°C, and equilibrate at room temperature for 10 minutes;

[0085] S22. Sample extraction and purification

[0086] Weigh 10.0g (accurate to 0.01g) sample; add 10mL acetonitrile, homogenize at high speed for 0.5min, add 4.0g anhydrous MgSO 4 After mixing with 1.0g NaCl, vortex at high speed for 1 min, and centrifuge at 10,000 r / min at 4°C for 5 min at high speed. Accurately pipette 2 mL of acetonitrile extract into a 10 mL plastic centrifuge tube, add 50 mg of N-propylethylenediamine (PSA) and 50 mg of C18 solid phase dispersion extraction purifier, vortex at high speed for 0.5 min, and centrifuge at 10,000 r / min at 4°C 5min. Take 1 mL of the supernatant, blow it to dryness with nitrogen at 30°C, and dissolve the residue wi...

experiment example 1

[0094] The methodological examination result of kit of the present invention

[0095] The kit prepared in Example 1 was identified according to the common verification procedures in this field, and the results are shown in Table 1.

[0096] The methodological testing result of test kit of the present invention of table 1

[0097]

[0098] The above results show that the accuracy, specificity, precision, sensitivity and stability of the "Carbofuran Biological Barcode Immunoassay Assay Kit" are fully qualified.

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Abstract

The invention relates to the technical field of food safety inspection immunoassay, in particular to a carbofuran bio-barcode immunoassay kit.The carbofuran bio-barcode immunoassay kit comprises a kit body containing a carbofuran standard, a carbofuran magnetic nanoprobe, a single-labeled colloidal gold nanoprobe, a carbofuran double-labeled colloidal gold nanoprobe, a DNA biochip and silver stain.The carbofuran bio-barcode immunoassay kit is capable of detecting carbofuran content of a sample to be detected simply, conveniently, quickly, sensitively, specifically and stably, good in experimental reproducibility and convenient to operate and produce.

Description

technical field [0001] The invention relates to the technical field of food safety detection immunoassay, in particular to a carbofuran biological barcode immunoassay assay kit and its application. Background technique [0002] Carbofuran is an irreversible cholinesterase inhibitor, which is highly toxic to humans, livestock, fish, birds, etc. It is generally processed into granules or seed coatings for soil or seed treatment. The drug has a long residual effect in the soil. Plants can absorb and conduct it through roots, stems, leaves or seeds, and accumulate more at the leaf margins. Eating the contaminated plants by mistake may cause poisoning. Carbofuran in soil may also contaminate surface and groundwater sources through runoff and infiltration. Therefore, the establishment of efficient and rapid carbofuran detection technology is of great significance for the monitoring of carbofuran residues in the environment and agricultural by-products, the accurate and rapid diag...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): G01N33/577G01N33/532
CPCG01N33/532G01N33/577
Inventor 金茂俊王静杜鹏飞金芬邵华佘永新郑鹭飞王珊珊
Owner INST OF QUALITY STANDARD & TESTING TECH FOR AGRO PROD OF CAAS
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