PCR primer sets, probe sets and kits for the detection of various respiratory viruses

Abstract

The invention discloses a PCR primer set for detecting multiple respiratory viruses, a probe set and a kit. All sequences of PCR primers are shown in SEQ ID NO.1-SEQ ID NO.18. Probes comprise at least two of molecular beacon probes for the nine respiratory viruses, wherein the two ends of the probes are provided with fluorophores and quenching groups respectively. All sequences are shown in SEQ ID NO.19-SEQ ID NO.27. The invention further discloses a PCR reaction solution containing the PCR primers and the probes and the kit containing the PCR reaction solution for at least one person. By means of the PCR primer set, the probe set and the kit, the nine respiratory viruses can be rapidly and accurately detected in a parting mode.

Description

technical field [0001] The invention relates to a PCR primer set, a probe set and a kit for detecting various respiratory viruses. Background technique [0002] Respiratory tract infection is one of the most common clinical diseases, which can be caused by a variety of pathogens, including bacteria, viruses, mycoplasma, chlamydia, etc. Among them, acute respiratory tract infection (Acute Respiratory Tract Infection, ARTI) is an important cause of illness and death in children worldwide. The World Health Organization (WHO) published an article in the "Lancet" magazine in 2005 and reported that in 2000-2003, among the 10.6 million children under the age of 5 who died each year, 54% of them died from infectious diseases, of which 54% died from pneumonia. Children accounted for 19%. ARTI is strictly defined as all respiratory infections, however, in reality, the acute lower respiratory tract accounts for the majority of severe respiratory diseases, accounting for 20% of all de...

AI Technical Summary

Problems solved by technology

[0004] With the development of the medical level, the above-mentioned traditional methods can no longer satisfy the detection of respiratory pathogens. Clinically, there is a need for a sensitive, specific, fast and simple method for the identification and detection of respiratory multi-pathogens, in order to be able to do better. Individualized treatment, and meet the needs of sanitation and epidemic prevention and epidemic control, and epidemiological research on infectious diseases

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