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Cleavers-containing curvularia culture medium and preparation method thereof

A technology of pig scorpion and culture medium, which is applied in the field of microbial cultivation, can solve problems such as waste, and achieve the effect of simple operation, simple formula and low cost

Active Publication Date: 2016-07-06
INST OF PLANT PROTECTION SHANDONG ACAD OF AGRI SCI
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

This is undoubtedly a huge waste for weed populations that contain a large amount of biomass resources

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0031] Embodiment 1 The preparation of medium provided by the present invention

[0032] Take 20g of pig's scorpion, remove the root and wash, manually chop until the stem and leaf fragments are less than 3 cm, boil the stem and leaves of pig's scorpion for 30 minutes, and filter the weed stem and leaves with gauze. Add 1.0g of peptone, 1.0g of glucose, 0.5g of magnesium sulfate heptahydrate, 0.5g of potassium dihydrogen phosphate, and add distilled water to 1000mL to adjust the pH to 6.5-8.0, and add 15-20g of agar to the juice obtained by filtration. The solution after constant volume was kept at 121°C, 1.05×10 5 Sterilize in a sterilizing pot under Pa for 20 minutes, and set aside after sterilization. According to the composition, the medium was named as Curvularia suis medium (Bedstraw curvularia medium, BSC).

Embodiment 2

[0033] Embodiment 2 Comparison of BSC culture medium of the present invention and other culture medium containing weeds

[0034] In this example, barnyardgrass, crabgrass, foxtail, sagebrush and amaranth were used as additional weeds at the same time, and a horizontal comparison was made with the culture medium added with pigtail. The cultured strain used in this embodiment is the model strain of Curvularia lunata: Curvularia lunae.

[0035] Table 1 The culture results of adding different weed media

[0036] culture medium Colony diameter (cm) Sporulation (10 9 ) CK 4.7±0.2 c 0.7±0.1 c pig scorpion 5.7±0.1 a 33.9±1.6 a barnyard grass 5.4±0.1 ab 11.3±3.6 b crabgrass 5.1±0.1 b 10.7±0.9 b foxtail 5.6±0.5 a 8.9±1.1 b Artemisia persicae 2.9±0.4 d 0.2 d Amaranth 2.8±0.2 d 0 e

[0037] Note: The values ​​in the table are mean ± standard error (n=4), different letters after the data in the same row indic...

Embodiment 3

[0039] The comparison of embodiment 3 BSC medium of the present invention and other commonly used medium

[0040] Potato dextrose medium (PDA): 200g potatoes, manually cut into potato pieces of about 2×2 cm, boiled in boiling water for 30 minutes, filtered through gauze, and retained the juice. Add 20 g of glucose and 20 g of agar to the juice obtained by filtration, and set the volume to 1000 ml. The solution after constant volume was kept at 121°C, 1.05×10 5 Sterilize in a sterilizing pot under Pa for 20 minutes, and set aside after sterilization.

[0041] Carrot medium (CA): Carrot 200g, artificially cut into carrot pieces about 2×2 cm, boiled in boiling water for 30 minutes, filtered with gauze, and retained the juice. Add 20 g of agar to the juice obtained by filtration, and set the volume to 1000 ml. The solution after constant volume was kept at 121°C, 1.05×10 5 Sterilize in a sterilizing pot under Pa for 20 minutes, and set aside after sterilization.

[0042]Corns...

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PUM

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Abstract

The invention belongs to the technical field of microorganism culture, and particularly relates to a cleavers-containing curvularia culture medium and a preparation method thereof.According to a formula for preparing 1000 ml of the culture medium, 20-160 g of cleavers, 1.0 g of peptone, 1.0 g of glucose, 0.5 g of magnesium sulfate heptahydrate, 0.5 g of monopotassium phosphate and 15-20 g of agar are taken, distilled water is added till the volume is 1000 ml, and the pH is regulated to be 6.5-8.0.By means of the cleavers-containing curvularia culture medium and the preparation method thereof, the curvularia culture cost is reduced; by adding common field grass into a culture medium, the fine culture medium widely applied to curvularia is developed.

Description

technical field [0001] The invention belongs to the technical field of microorganism culture, and in particular relates to a culture medium for Curvularia suis containing the scorpion and a preparation method thereof. Background technique [0002] Curvularia was proposed by Boedijn in 1933. Its main features are: the conidia have 3 or 4 diaphragms, and the middle cells are usually asymmetrically enlarged so that the spores look obviously curved. Curvularia is an important plant pathogen, which mainly parasitizes corn, wheat, barley, oats, rye, rice, sorghum, sugarcane and other gramineous crops and turfgrass, causing plant leaf spots, bacterial wilt, Seed discoloration and other symptoms. Curvularia can cause maize yellow spot, and the conidia of the diseased fungus in the diseased residue can overwinter on the surface and serve as the source of primary infection in the following year. Curvularia lunata can infect tall fescue used in turf, and exogenous salicylic acid can...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12N1/14C12R1/645
CPCC12N1/14
Inventor 李健李美高兴祥房锋
Owner INST OF PLANT PROTECTION SHANDONG ACAD OF AGRI SCI
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