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Application of rhodopseudomonas palustris fermentation liquor to rice blast prevention and control

A fermentation technology of Rhodopseudomonas palustris and bacteria, which is applied in the application field of Rhodopseudomonas palustris fermentation liquid in the prevention and control of rice blast, and achieves the effects of short cultivation time, low cost and simple production process

Active Publication Date: 2016-07-27
HUNAN PLANT PROTECTION INST
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

The photosynthetic bacterium Rhodopseudomonas palustris fermentation liquid has not been reported in the prevention and control of rice blast

Method used

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  • Application of rhodopseudomonas palustris fermentation liquor to rice blast prevention and control
  • Application of rhodopseudomonas palustris fermentation liquor to rice blast prevention and control

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0030] A preparation method of Rhodopseudomonas palustris LY-6 fermented liquid of the present invention, comprises the following steps:

[0031] (1) Activation: Culture the preserved species of Rhodopseudomonas palustris LY-6 strain according to the double-layer plate culture method until red single colonies appear; the medium used is solid medium for photosynthetic bacteria, and the formula is: ( NH 4 ) 2 SO 4 0.1wt%, MgSO 4 0.02wt%, NaHCO 3 0.5wt%, K 2 HPO 4 0.05wt%, NaCl0.02wt%, yeast extract 0.15wt% and agar 1.5wt%, pH=7.0.

[0032] (2) Seed culture: Put the red single colony cultivated in step (1) into a 120mL serum bottle, and use photosynthetic bacteria The liquid medium is cultivated to the logarithmic growth phase; the medium used is a photosynthetic bacteria liquid medium, and the formula is: (NH 4 ) 2 SO 4 0.1wt%, MgSO 4 0.02wt%, NaHCO 3 0.5wt%, K 2 HPO 4 0.05wt%, NaCl 0.02wt%, yeast extract 0.15wt% (all mass fractions), pH=7.0.

[0033] (3) Producti...

Embodiment 2

[0037] Example 2: Prevention and control of leaf blast in the field with Rhodopseudomonas palustris LY-6 fermented liquid

[0038] The fermented liquid (concentration 5 * 10 9 cfu / mL), the inactivated fermentation broth prepared in Comparative Example 1, and chemical pesticides (Diaobenling, 120mL / mu) were sprayed on the rice leaves in the late tillering stage, and each treatment was repeated 3 times, 20L per mu, Spray once every 15 days and apply 3 times in total. 15 days after the last spraying, the rice leaf disease index was calculated by sampling according to the 5-point method, and the respective control effects were calculated.

[0039] figure 1 It is a comparison chart of the control effects of the inactivated fermentation broth of Comparative Example 1, the fermentation broth of Example 1, and chemical pesticides on rice leaf blast. A and B represent significant differences (Pfigure 1 It can be seen that the control effects of applying inactivated fermentation broth...

Embodiment 3

[0040] Example 3: Prevention and control of panicle blast in the field of Rhodopseudomonas palustris LY-6 fermented liquid

[0041] The fermented liquid (concentration 5 * 10 9 cfu / mL), the inactivated fermentation broth prepared in Comparative Example 1, and chemical pesticides (Diaobenling, 120mL / mu) were sprayed on the rice leaves at the heading stage, and each treatment was repeated 3 times, 20L per mu, Spray once every 15 days, and apply 2 times in total. After 15 days, the rice panicle disease index was calculated by sampling according to the 5-point method, and the respective control effects were calculated.

[0042] figure 2 It is a comparison chart of the control effects of the inactivated fermentation broth of Comparative Example 1, the fermentation broth of Example 1, and chemical pesticides on rice panicle blast, A and B represent significant differences (P<0.01). The control effects of applying inactivated fermentation broth, fermentation broth, and pesticides...

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Abstract

The invention discloses application of rhodopseudomonas palustris fermentation liquor to rice blast prevention and control.The rhodopseudomonas palustris fermentation liquor is obtained by culturing rhodopseudomonas palustris LY-6.Rhodopseudomonas palustris is used for preventing and controlling rice blast for the first time, and the rhodopseudomonas palustris fermentation liquor has the advantages of being environmentally friendly, nontoxic to human and livestock, free of phytotoxicity to crops, easy and convenient to apply and the like.

Description

technical field [0001] The invention relates to the technical field of biopesticides, in particular to the application of a fermented liquid of Rhodopseudomonas palustris in preventing and controlling rice blast. Background technique [0002] Rice blast is one of the important diseases of rice. The disease is widely distributed. It occurs in more than 80 countries and regions in the world, and it also occurs in the north and south rice regions of my country. In areas where the disease is endemic, the yield is generally reduced by 10% to 20%, and in severe cases it can reach 40% to 50%. Especially heavy fields may even fail to harvest. For the control of rice blast, chemical control is still the main method at present, but due to the increase of drug resistance and untimely discovery, the application of pesticides is extremely unscientific and reasonable, and the dosage and frequency of pesticide applications seriously exceed the standard, causing major problems such as envir...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A01N63/02A01P3/00
CPCA01N63/10
Inventor 刘勇张德咏陈岳谭新球苏品肖友伦李小娟
Owner HUNAN PLANT PROTECTION INST
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