81 results about "Rhodopseudomonas species" patented technology

The invention relates to a microbe germ composition, a cyanophyte biological germ leaven which contains the microbe germ, a cyanophyte fermentation nutrient, a cyanophyte biological organic fertilizer and a method for preparation, wherein the microbe germ composition contains plant lactobacillus, bacillus subtilis and swamp Rhodopseudomonas, the cyanophyte biological germ leaven is prepared by microbe germ composition and base, the cyanophyte fermentation nutrient is prepared by cyanophyte biological germ leaven and cyanophyte, the cyanophyte biological organic fertilizer is prepared by cyanophyte fermentation nutrient, organics base and water-loss reducer. The microbe germ composition is provided with the ability for fermenting solid cyanophyte, which can transform nutriment in the cyanophyte into the material for crops. The cyanophyte fermentation nutrient can be superior animal feed. The cyanophyte biological organic fertilizer is provided with crop pest control effect, which adds the soil organics content and improves nutrient utility to accelerate the plant growth belonging to natural superior organics fertilizer.

This invention relates to a kind of photosynthetic bacterium that has a high assimilation utility to nitrite nitrogen. More specifically, this invention provides a Rhodopseudomonas palustris strain that can assimilate nitrite nitrogen, and its r ejuvenation, preservation, biological detection and application. The photosynthetic bacterium is a kind of biological water purifier and biological fertilizer, and can be used in controlling the content of nitrite nitrogen in water body for aquaculture, improve environmental pollution, and eliminate cinnamic acid and benzoic acid. The photosynthetic bacterium can be used as nitrogen-fixing biological fertilizer and cucumber growth promoter.

The invention discloses a making method of polyvalent composite microbe fertilizer, which comprises the following steps: culturing bacteria under each fitful culture medium to prepare; allocating ferment culture medium; setting constant volume of ferment tank when the pH value is 7.4; sterilizing; weighing each 10% gel-shaped bacillus and blown-ball Azotobacter to inoculate at 30 deg.c with the ventilation quantity at 1: 0.5; stirring to culture for 12h; weighing 10% thin yellow streptomycete to culture for 10h under the same condition after detecting; culturing 7% huge bacillus and bacillus subtilis for 10h; culturing 10% brewery saccharomycete for 10h; weighing 30% composite bacteria of red coccus, capsula Rhodopseudomonas and marsh capsula Rhodopseudomonas and prunosus red coccus to culture at 30 deg.c for 10h without stirring; being first choice fertilizer to manufacture green pollution-free crop.

The invention discloses compound bactericide for treating sewage and a sewage treatment method thereof, and belongs to the technical field of sewage treatment. The compound bactericide comprises the following active ingredients from following strains: rhodopseudomonas, anaerobic ammonium oxidation bacteria, candida utilis, thiobacillus denitrificans, white-rot fungi, lactobacillus plantarum, bifidobacterium, lactobacillus acidophilus, green nonsulfur bacteria, phosphorus-accumulating bacteria, aspergillus oryzae, nitrosomas, streptomyces, bacillus subtilis and propionibacterium acidipropionici. The invention also provides the method using the compound bactericide to treat the sewage. The compound bactericide and the sewage treatment method have the advantages that the effect of treating sewage is better, the technology is simple, the implementing is easy, the operability is strong, the pollution to environment is avoided, the industrial requirements are met, and the application prospect is better.

The invention discloses a Rhodopseudomonas strain. The name of the Rhodopseudomonas strain is Rhodopseudomonas PSB-06, and the collection number is CCTCC NO:M2012518. The invention also discloses a biocontrol microbial inoculum which is obtained through the activation, seed culture and production culture of the strain. The preparation of the biocontrol microbial inoculum comprises the multiple steps of activation, seed culture and production culture. The invention also discloses a Rhodopseudomonas strain biocontrol fermentation liquid which is prepared from the biocontrol microbial inoculum through fermentation and centrifugation. Each of the Rhodopseudomonas strain, the biocontrol microbial inoculum and the biocontrol fermentation liquid can be used in antagonizing phytophthora capsici or controlling phytophthora blight of pepper.

The invention discloses a method of treating and recycling food processing waste water by using a photosynthetic bacterium, which belongs to the technical field of sewage treatment. The method comprises the following steps: adding a micro-molecular carbon source substance into food processing waste water until a final concentration of the micro-molecular carbon source substance is 160 to 2000 mg/L and adding a micro-molecular nitrogen source substance into the food processing waste water until a final concentration of the micro-molecular nitrogen source substance is 55 to 1000 mg/L; adjusting the pH value of the waste water to be 7.0 to 9.0; then adding Rhodopseudomonas palustris in a logarithmic phase into the waste water until a final concentration of Rhodopseudomonas palustris is 240 to 2000 mg/L; carrying out treatment at a temperature of 25 to 30 DEG C for 96 to 120 h; and controlling externally applied illumination intensity to be 500 to 1500 lux by using an electric incandescent lamp and controlling the concentration of dissolved oxygen in the waste water to be 0.5 to 1.0 mg/L. The method provided by the invention is simple and easily practicable, enables process flow to be simplified and a recycling degree of sewage to be improved, the problem of secondary pollution in traditional sewage treatment processes is avoided, and energy consumption is lowered down.

The invention relates to a composite microbial preparation for promoting the growth of rape and a preparation method thereof. The composite microbial preparation for promoting the growth of the rape is characterized by comprising 20-40 parts by weight of strain culturing substance and 60-80 parts by weight of microbial carrier, wherein the strain culturing substance comprises the following raw materials in parts by weight: 2-8 parts of bacillus subtilis, 2-8 parts of bacillus megaterium, 5-10 parts of rhodopseudomonas palustris and 1-4 parts of azotobacter chroococcum. The preparation method of the composite microbial preparation for promoting the growth of the rape comprises the following steps: culturing 2-8 parts of bacillus subtilis, 2-8 parts of bacillus megaterium and the rhodopseudomonas palustris together, culturing the azotobacter chroococcum independently, mixing and fermenting. The invention has the advantages of promoting the growth and the propagation of beneficial microorganisms, increasing the absorption of the root system of the rape to nitrogen, phosphorus, potassium and other organic substances and promoting the normal development of the root system, thus promoting the growth of the rape and reducing the applied chemical fertilizer.

The invention discloses a fish protein polypeptide biological bacterial fertilizer, which is composed of the following raw materials in parts by weight: 15-17 parts of deep sea fish, 4-6 parts of bean cake powder, 7-9 parts of rice bran, 6-8 parts of molasses, 0.045-0.05 part of strain and 50-60 parts of water, wherein the strain comprises saccharomyces cerevisiae and rhodopseudomonas palustris of which bacteria amount ratio is (1-12):(0.8-1.0). The beneficial effects of the fish protein polypeptide biological bacterial fertilizer are as follows: (1) quick action: after the fertilizer is used for 2-3 days, new roots are obviously increased, fruits are remarkably enlarged with bright color and lustre and deep green leaves; (2) the fertilizer can be used for improving fruit setting rate, reducing deformity fruits, improving single fruit weight, and realizing completely filled fruits and excellent mouthfeel; (3) the fertilizer can effectively relieve root senescence, soil compaction and salinization of soil caused by long-term fertilization of a fertilizer, and strengthen the capability of continuous cropping resistance, disease resistance and cold tolerance of crops; and (4) the fertilizer is fertilized to improve the use ratio of the fertilizer.

The invention discloses a production method of a bdellovibrio bacteriovorus preparation. The production method comprises the following: (1) preparing host bacteria mixed suspension: taking rhodopseudomonas sphaeroides P4 or rhodopseudomonas palustris 101 in photosynthetic bacteria and E.Coli600 as mixed host bacteria for producing and culturing bdellovibrio bacteriovorus; (2) preparing a culture solution; (3) carrying out a fermentation process of the bdellovibrio bacteriovorus. The viable count in the prepared bdellovibrio bacteriovorus preparation is obviously improved and a lot of plaques can be produced, so that the use effect of the bdellovibrio bacteriovorus preparation is improved, and the cracking activity, stability and disease prevention and treatment effect of the bdellovibrio bacteriovorus are enhanced.

A method for reinforcing flocculation performance of rhodopseudomonas faecalis of light-fermentative bacteria relates to a method for reinforcing flocculation performance of rhodopseudomonas faecalis and aims to solve the problems that flocculation performance of existing light-fermentative cells is poor, and hydrogen generation of a light-fermentative hydrogen generation reactor is low in efficiency due to continuous loss of biomass live-weight. The method includes steps of firstly, seeding 10% of rhodopseudomonas faecalis RLD-53 bacterium liquid to a growth culture medium, disposing the growth culture medium on a swing bed of 120rpm, culturing in continuous light with illumination intensity of 150W/m2 at the temperature of 35 DEG C for 16 hours, and obtaining seed liquid; secondly, seeding the seed liquid obtained in the first step to a hydrogen generation culture medium containing L-cysteine according to 10% seeding quantity, disposing the hydrogen generation culture medium on the swing bed of 120rpm, and culturing in continuous light at the temperature of 35 DEG C for 72 hours, and obtaining bacteria suspension containing flocculating constituents. The method can effectively improve flocculation performance of light-fermentative cells and realize flocculation growth of the light-fermentative cells and can be used for the field of light-fermentative hydrogen generation.

The invention discloses a pretreatment method for enhancing anaerobic sludge digestion by virtue of a compound microorganism bacterium agent. The pretreatment method comprises the following steps: concentrating residual sludge, adding a compound microorganism bacterium agent, performing stirring reaction, inoculating the pretreated residual sludge into an anaerobic digestion pond according to a certain ratio, and performing mesophilic anaerobic digestion on anaerobic sludge, wherein the compound microorganism bacterium agent is prepared by mixed fermentation of 5%-20% of each of bacillus subtilis, streptomyces albus, streptomyces griseus, candida utilis, aspergillus sojae, lactobacillus bulgaricus, lactococcus lactis, rhodopseudomonas palustris, rhodopseudomonas capsulate and rhodopseudomonas spheroides, the total viable count is not less than 2*108/ml, and the pH value of the compound microorganism bacterium agent is 3-4. According to the method, the sludge hydrolysis process can be accelerated, the sludge retention time can be shortened, the biological gas production rate can be increased, and the anaerobic digestion efficiency can be improved.

The invention discloses a method for preparing rhodopseudomonas palustris for feed by utilizing white maize meal as a raw material. The preparation of rhodopseudomonas palustris for feed can be effectively solved, so that the requirement of the feed breeding industry for feed production is met. According to the technical scheme, the method comprises the following steps: preparing a strain, culturing the strain, preparing a liquid fermentation culture medium, inoculating, performing fermentation cultivation, performing carrier adsorption and drying. The method disclosed by the invention has the advantages that 1, the raw materials of the fermentation medium mainly are white maize meal and other materials which are rich in resources, low in price and simple and readily available; 2, the fermentation medium is simple in formula, fermentation production in an automatic liquid fermentation tank is realized, the operation is convenient, the culture is convenient, the growth speed is high, and the fermentation period is greatly shortened and is only 72-96 hours; 3, the rhodopseudomonas palustris for feed can be prepared; 4, the rhodopseudomonas palustris for feed is good in application effect and outstanding in economic and social benefits; and 5, the microbial strain rhodopseudomonas palustris AS1.2352 permitted in the Department of Agriculture in China is used for fermentation production, the safety is high, and the rhodopseudomonas palustris is a green additive for feed.

The invention discloses a method for producing an aerobic fermentation photosynthetic bacteria livestock culturing feed additive, belonging to the field of microbial fermentation. The technical problem to be solved of the invention is to provide a method for producing an aerobic fermentation photosynthetic bacteria livestock culturing feed additive which has stable quality and low energy consumption. The adopted technical scheme comprises the followings steps of: performing strain amplification culture, inoculating marsh rhodopseudomonas in an amount 8-12 percent based on the volume of an amplification culture medium, and fermenting at the temperature 27 DEG C and the light intensity 1,000-3,000 lux for 3-4 days to obtain a strain culture fluid; performing fermentation production: inoculating bacteria in an amount 10-18 percent based on the volume of the fermentation culture medium at the temperature 29 DEG C, stirring at the speed 100 r/min, keeping the solved oxygen at 1.8 mg/L in atank with sterile air, and keeping the internal pressure of the tank at 0.02-0.04MPa; and fermenting for 5 days under the dark condition to obtain the photosynthetic bacteria livestock culturing feedadditive. Compared with the prior art, the method has the advantage that: the energy consumption of fermentation production is lowered.

The invention relates to a papermaking wastewater bio-decolorization method. The papermaking wastewater bio-decolorization method belongs to a papermaking wastewater depth treatment decolorization technology. The papermaking wastewater bio-decolorization method is characterized in that 1, yielding water which is high-yield pulping papermaking integrated wastewater passing through thick and thin grilles and being subjected to secondary treatment and coagulation agent addition treatment is utilized as a treatment object, wherein the chemical oxygen demand (COD) of the yielding water is less than or equal to 150mg/L and the chroma is less than or equal to 80 times; 2, the papermaking wastewater bio-decolorization method utilizing active carbon as a decolorization bacterium carrier is carried out in a special airlift biochar circulation reactor, and in other words, active carbon, secondary yielding water and compressed air are put in the same reactor so that decolorization depth treatment is realized; and 3, in a decolorization process with addition of white rot fungi and rhodopseudomonas, other chemical preparations are not needed, wherein a COD removal rate can reach above 50%; a chroma clearance rate can reach above 80%; and a higher clearance rate can be obtained when a coagulant addition amount is increased and reaction residence time of treated wastewater in a reactor is prolonged. The papermaking wastewater bio-decolorization method utilizes active carbon as a decolorization microbe carrier and compressed air as a power source of oxygen and active carbon bed expansion, and realizes flowing of decolored wastewater and compressed air in active carbon, a dynamic balance between active carbon adsorption and adsorbed substance biodegradation, continuous water yielding and stable water-yielding operation.

The invention discloses a microbial compound preparation used for repairing heavy metal contaminated soil. The compound microbial preparation is characterized by comprising rhodopseudomonas palustrisDSM5859 fungicide, lactobacillus plantarum NJ2205 fungicide, bacillus subtilis BNCC188062 fungicide and immobilized high temperature resistance alpha-amylase. The invention further discloses a preparation method and application of the microbial preparation. According to the microbial preparation, the immobilized high temperature resistance alpha-amylase is applied to the field of soil remediationfor the first time, the rhodopseudomonas palustris DSM5859 fungicide, the lactobacillus plantarum NJ2205 fungicide, the bacillus subtilis BNCC188062 fungicide and the immobilized high temperature resistance alpha-amylase are mixed for preparing the microbial enzyme preparation for the first time, the removal rate of Cd, Cr, Cu, Ni and Zn reaches 85% or above, and the preparation is environmentallyfriendly, cannot cause secondary pollution, and is beneficial for large-scale utilization and popularization for oil.

The invention relates to a microbe decomposing agent for livestock and poultry manure waste, and a preparing method and applications thereof. The decomposing agent includes paenibacillus polymyxa powder, rhodopseudomonas rutila powder, trichoderma reesei powder, endomycopsis fibuligera powder and European verbena herb powder. The total number of effective living bacteria in the decomposing agent is 2-2.5*10<8> per gram, enzyme production activity is good and product quality and properties are stable. Bacteria, fungi and a traditional Chinese medicine, which are matched, are adopted as raw materials of the decomposing agent. Through controlling a microbe fermentation process, concentration and drying steps are performed to obtain microbe powder the water content of which is within 10%, and then the microbe powder is mixed with the traditional Chinese medicine that is the European verbena herb powder. The decomposing agent has advantages of rapid temperature rising, good decomposition effects and stable quality and properties when the decomposing agent is used for treating the livestock and poultry manure waste.

The invention discloses a facultative anaerobic Rhodopseudomonas strain which is collected in China Center for Type Culture Collection, and the collection number is CCTCC NO:M2012366. The Rhodopseudomonas strain can inhibit Pseudoperonospora cubensis Rostov and promote cucumber growth. When in use, a photosynthetic bacteria liquid can be directly sprayed, or prepared into a fermentation liquid and sprayed. The preparation method of the photosynthetic bacteria liquid and photosynthetic bacteria fermentation liquid comprises the following steps: activating the Rhodopseudomonas strain; inoculating the activated and cultured single colony into a serum bottle, and carrying out seed culture; carrying out production culture to obtain the photosynthetic bacteria liquid; and centrifugating the photosynthetic bacteria liquid, collecting the supernate, and filtering through a biofilter to obtain the photosynthetic bacteria fermentation liquid. The method has the advantages of low production cost, favorable antagonistic effect, high efficiency, no toxicity, environmental protection and the like.

The invention discloses an application of photosynthetic bacterium and bacillus subtilis mixed bacterial liquid in preparing microbial inoculum for preventing and controlling red mouth disease of turbot. The application is characterized in that photosynthetic bacterium culture medium and bacillus subtilis culture medium are mixed according to the ratio of 1:1 on the basis of the number of bacteria; the photosynthetic bacterium is at least one of rhodopseudomonas, rhodopseudomonas palustris and capsule erythrocyte. The application method of the bacterial liquid comprises the following steps: spraying bactericide into a water body to ensure that the final concentration of the photosynthetic bacterium and bacillus subtilis mixed bacterial liquid is between 10 and 107cfu/ml, or dipping feed in the photosynthetic bacterium and bacillus subtilis mixed bacterial liquid, wherein the concentration of the photosynthetic bacterium and the bacillus subtilis in the mixed bacterial liquid is between 10 and 107cfu/mL. The bactericide is effective, environement-friendly and without public nuisance, has excellent application prospect, and has important guidance meaning for preventing overuse of antibiotics.

The invention belongs to the technical field of microorganisms and discloses a compound microorganism culture for preparing carotenoid. The compound microorganism culture comprises bacillus subtilis,rhodopseudomonas capsulate and rhodotorula glutinis. The compound microorganism culture increases yield of carotenoid and lowers culture cost.

The invention discloses a preparation method of a high-concentration microalgae living cell long-acting preservative. The preparation method comprises the steps of fermenting and culturing rhodopseudomonas capsulate and halophila campisalis in a glutamate culture medium to obtain bacterial sludge, embedding the bacterial sludge into sodium alginate carrier particles, and continuously adsorbing nutrient matrix powder containing inorganic salts, vitamins, organic carbon sources and the like into the carrier particles to obtain photosynthetic bacterium carrier particle nutrient salt mixed particles, and carrying out spray drying and powder homogenization to obtain the high-concentration microalgae living cell long-acting preservative. The preservative is simple to use, only a certain amount of preservative powder needs to be added into the concentrated algae liquid, bacteria are increased in the embedded particles, and the generated metabolites can maintain the ecological balance of an algae concentrated solution. The method is simple and feasible, and the vigor of concentrated algae living cells can be maintained for 10-30 days in spring, summer, autumn and autumn without decay.

The invention provides a decomposing inoculant for corn straw and a preparation method of the decomposing inoculant. The decomposing inoculant has the advantages that the decomposing inoculant does not contain fungi and can effectively promote the decomposing of the corn straw without supplementing extra nitrogen sources; Bacillus licheniformis, Bacillus megaterium, Streptomyces cellulosae and Rhodopseudomonas palustris in the complex microbial inoculant has good decomposing ability to livestock and poultry manure and the corn straw, and the Bacillus and the Streptomyces can also generate metabolite inhibiting the fungi and can effectively inhibit pathogenic bacteria in soil and on the corn straw returning to the field so as to achieve a disease control effect.

This invention is associated with the microbe fermentation. It is the mothod of producing coenzyme Q10 by fermentation. The existing method adopts rhodopseudomonas and the production must use light and the duration is very long, and cost is very high. This invention uses agrobacterium to produce coenzyme Q10 by fermentation, so the growth does not need light. The it can realize high density fermentation with low cost.

The invention relates to a preparation method for a plantation fertilizer special for Chinese yews. The method comprises the following steps, firstly, tung oil cakes are added in a pulverizer, pulverized and added with water, and the water content is kept in 60-65%; secondly, by weight, 0.15-0.2% of trichoderma viride, 0.15-0.2% of aspergillus oryzae, 0.05-0.1% of bacillus subtilis and 0.05-0.1%of rhodopseudomonas palustris are added in the above tung oil cake residues, the mixture is mixed uniformly and subjected to compost primary fermentation, and the fermentation temperature is controlled at 60-65 DEG C; thirdly, when the primary fermentation temperature is lowered to 40 DEG C, by weight, 0.05-0.1% of azotobacter chroococcum, 0.05-0.1% of bacillus megaterium and 0.05-0.1% of bacillus mucilaginosus are added in the compost and the mixture is subjected to secondary fermentation, and the fermentation temperature is kept under 50 DEG C; fourthly, by weight, 85-95% of the fermented organic fertilizer and 5-15% of the potassium sulfate composite fertilizer are mixed uniformly. The provided plantation fertilizer special for Chinese yews can promote growth of Chinese yews and does not pollute the environment.