Ferment method for producing coenzyme Q10
A fermentation method and coenzyme technology are applied in the field of microbial fermentation to achieve the effects of reducing fermentation costs, requiring low fermentation conditions and moderate fermentation temperature
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[0015] Inoculate Agrobacterium sp. slant LQ10 (Agrobacterium sp.) preserved in paraffin oil into a 5ml test tube culture medium, culture at 30°C for 12 hours, and then transfer to a 400ml medium Erlenmeyer flask and culture at 30°C for 12 hours. Medium composition of two-stage seed culture (g / L): glucose 5g, peptone 5g, beef extract 5g, yeast powder 1g, MgSO 4 ·7H 2 O 0.49g, agar 20g, sterilized at 121°C for 15min.
[0016] Then 4 liters of culture solution are inserted into 5 liters of full-automatic fermenters for fermentation, culture medium (g / L): glucose 28g, sucrose molasses 50g, yeast powder 5g, corn steep liquor 28g, (NH 4 ) 2 sO 4 7g, KH 2 PO 4 0.4g, Na 2 HPO 4 12H 2 O 1.5g, MgSO 4 ·7H 2 O 0.3g, add 0.01% defoamer to sterilize at 121℃ for 15min. The fermentation temperature is 30°C, the dissolved oxygen content is 30%, the stirring speed is 400rpm, and the pH value is 7.2. After 48 hours of cultivation, the pH value is adjusted to 6.5 to facilitate the acc...
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