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Ferment method for producing coenzyme Q10

A fermentation method and coenzyme technology are applied in the field of microbial fermentation to achieve the effects of reducing fermentation costs, requiring low fermentation conditions and moderate fermentation temperature

Inactive Publication Date: 2006-12-27
广州市微生物研究所 +1
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AI Technical Summary

Problems solved by technology

[0003] The purpose of the present invention is to overcome existing coenzyme Q 10 Problems in the growth method, providing a method that does not require light and can be fermented at high density to produce coenzyme Q 10 Methods

Method used

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Examples

Experimental program
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Effect test

Embodiment 1

[0015] Inoculate Agrobacterium sp. slant LQ10 (Agrobacterium sp.) preserved in paraffin oil into a 5ml test tube culture medium, culture at 30°C for 12 hours, and then transfer to a 400ml medium Erlenmeyer flask and culture at 30°C for 12 hours. Medium composition of two-stage seed culture (g / L): glucose 5g, peptone 5g, beef extract 5g, yeast powder 1g, MgSO 4 ·7H 2 O 0.49g, agar 20g, sterilized at 121°C for 15min.

[0016] Then 4 liters of culture solution are inserted into 5 liters of full-automatic fermenters for fermentation, culture medium (g / L): glucose 28g, sucrose molasses 50g, yeast powder 5g, corn steep liquor 28g, (NH 4 ) 2 sO 4 7g, KH 2 PO 4 0.4g, Na 2 HPO 4 12H 2 O 1.5g, MgSO 4 ·7H 2 O 0.3g, add 0.01% defoamer to sterilize at 121℃ for 15min. The fermentation temperature is 30°C, the dissolved oxygen content is 30%, the stirring speed is 400rpm, and the pH value is 7.2. After 48 hours of cultivation, the pH value is adjusted to 6.5 to facilitate the acc...

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PUM

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Abstract

This invention is associated with the microbe fermentation. It is the mothod of producing coenzyme Q10 by fermentation. The existing method adopts rhodopseudomonas and the production must use light and the duration is very long, and cost is very high. This invention uses agrobacterium to produce coenzyme Q10 by fermentation, so the growth does not need light. The it can realize high density fermentation with low cost.

Description

technical field [0001] The invention relates to the field of microbial fermentation, in particular to a fermentation production of coenzyme Q 10 Methods. Background technique [0002] coenzyme Q 10 There are three kinds of production methods of animal and plant tissue extraction, chemical synthesis and microbial fermentation. Among them, the animal and plant extraction method has high cost and is not suitable for modern industrial production; the chemical synthesis method has complex reactions, many by-products, and high cost. Microbial fermentation is currently only used in Japan. However, the bacterial species used by Japanese companies is Rhodopseudomonas sp., and light must be used during fermentation production, which takes a long time to cultivate, thus increasing production costs. Contents of the invention [0003] The purpose of the present invention is to overcome existing coenzyme Q 10 Problems in the growth method, providing a method that does not require li...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12P7/26C12R1/01
Inventor 董加喜张高贤陆勇军熊辉华张添元李运南
Owner 广州市微生物研究所
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