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A microbe decomposing agent for livestock and poultry manure waste and a preparing method and applications thereof

A microbial decomposing agent and waste technology, which is applied in the preparation and application of organic fertilizers, organic fertilizers, etc., can solve the problem of poor stability of decomposing effect of livestock and poultry manure waste, failure to fully consider the ability of bacteria to produce enzymes, bacteria Unreasonable combination with strains and other problems, to achieve the effect of prolonging the shelf life of products, good promotion and use value, and beneficial to plant growth

Inactive Publication Date: 2017-06-20
新疆肥力沃生物工程有限公司
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, in the existing decomposing agent products produced, most of the decomposing agent products are bacterial agent products developed and produced for decomposing livestock and poultry manure or its mixture with livestock and poultry manure waste under stacking conditions. The low bacterial count and too small enzyme activity make it difficult to meet the actual needs of market applications. At the same time, the selection of bacteria and strain combinations for decomposer products is not reasonable, and the enzyme production ability of the bacteria itself and the strains are not fully considered. The interaction between each other has symbiotic, mutual and antagonistic effects on the product
In particular, there are few types of fungi, and the stability of the decomposing effect of livestock and poultry manure waste in use is poor

Method used

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  • A microbe decomposing agent for livestock and poultry manure waste and a preparing method and applications thereof
  • A microbe decomposing agent for livestock and poultry manure waste and a preparing method and applications thereof
  • A microbe decomposing agent for livestock and poultry manure waste and a preparing method and applications thereof

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0031] Example 1 Preparation of Paenibacillus polymyxa powder:

[0032] A. Transfer the freeze-dried Paenibacillus polymyxa strains to the prepared test tube slope in a sterile environment, control the temperature at 35°C, cultivate for 36 hours, then transfer to eggplant-shaped bottles for 30 hours, check After confirming that there is no miscellaneous bacteria, it is used to inoculate the fermenter; the concentration of each component of the medium in the eggplant-shaped bottle is as follows: tryptone 8-10g / L, yeast extract 3-5g / L, sodium chloride 3-5g / L, solvent for water;

[0033] B. Preparation of medium material: The medium material in the fermenter needs to be proportioned according to the ratio of 0.5g of glucose, 0.5g of beef extract, 0.5g of peptone and 0.5g of sodium chloride per liter of water, mixed and dissolved in water, and transported to inside the fermenter;

[0034] C. Sterilization and cooling: put steam into the fermenter, start stirring, keep warm for 3...

Embodiment 2

[0040] Example 2 Preparation of Rhodopseudomonas rhodopsin powder:

[0041] A. Transfer the freeze-dried Rhodopseudomonas strains to the pre-prepared test tube slope in a sterile environment, control the temperature at 30-35°C, cultivate for 36 hours, and then transfer to an eggplant bottle for 32 hours. After checking and confirming that there is no miscellaneous bacteria, it is used for fermenter inoculation; the concentration of each component of the medium in the eggplant-shaped bottle is as follows: tryptone 8-10g / L, yeast extract 3-5g / L, sodium chloride 3-5g / L , the solvent is water;

[0042] B, prepare culture medium material: culture medium material in the fermenter needs to be configured with the proportioning of glucose 5g, yeast extract 2g, peptone 1g, soybean meal powder 5g and potassium dihydrogen phosphate 0.5g according to every liter of water, so that it is dissolved in water, into the fermenter;

[0043] C. Sterilization and cooling: put steam into the ferme...

Embodiment 3

[0049] Example 3 Preparation of Trichoderma reesei powder:

[0050] A. Preparation of culture medium mixture: according to the proportioning by mass percentage, the proportioning of the materials is 70% of bran, 15% of soybean meal, and 15% of corn flour. 0.5% magnesium sulfate heptahydrate, 3.5% iron sulfate, 0.2% potassium dihydrogen phosphate, and distilled water to make the water content reach 60%, and the trace elements are dissolved with water and added together with water ;

[0051] B. Sterilization and cooling: heat up the medium mixture material obtained in the process of A to 121°C, keep the temperature at 121°C and keep the pressure for 1 hour, then start to ventilate and cool to the interlayer of the fermentation tank, and cool to 30°C;

[0052] C, inoculation: after mixing Trichoderma reesei spore powder and 1 kg flour, inoculate on the culture medium mixture material in the fermentation tank after the high temperature and high pressure sterilization that obtains...

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Abstract

The invention relates to a microbe decomposing agent for livestock and poultry manure waste, and a preparing method and applications thereof. The decomposing agent includes paenibacillus polymyxa powder, rhodopseudomonas rutila powder, trichoderma reesei powder, endomycopsis fibuligera powder and European verbena herb powder. The total number of effective living bacteria in the decomposing agent is 2-2.5*10<8> per gram, enzyme production activity is good and product quality and properties are stable. Bacteria, fungi and a traditional Chinese medicine, which are matched, are adopted as raw materials of the decomposing agent. Through controlling a microbe fermentation process, concentration and drying steps are performed to obtain microbe powder the water content of which is within 10%, and then the microbe powder is mixed with the traditional Chinese medicine that is the European verbena herb powder. The decomposing agent has advantages of rapid temperature rising, good decomposition effects and stable quality and properties when the decomposing agent is used for treating the livestock and poultry manure waste.

Description

technical field [0001] The invention belongs to the field of microbial preparations, and relates to a microbial decomposing agent for livestock and poultry manure waste, a preparation method and application thereof. Background technique [0002] The annual production of various livestock and poultry manure wastes in my country is as high as billions of tons. In order to deal with these livestock and poultry manure wastes, many farmers directly discharge them into rivers, lakes and seas. Livestock and poultry manure waste not only pollutes the environment, but also seriously wastes resources and destroys ecology. In order to fundamentally solve this contradiction, my country has carried out research on "recycling of livestock and poultry manure waste", the main purpose of which is to select and use a good decomposing agent for livestock and poultry manure waste to realize the recycling of livestock and poultry manure waste. The fermentation of bio-organic fertilizer is carrie...

Claims

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Application Information

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IPC IPC(8): C12N1/20C12N1/14C05F11/08C05F15/00
CPCC12N1/20C05F3/00C05F11/08C12N1/14C05F17/00
Inventor 马金平王娟
Owner 新疆肥力沃生物工程有限公司
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