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Rhodopseudomonas strain, biocontrol microbial inoculum and biocontrol fermentation liquid, and corresponding preparation methods and application thereof in controlling phytophthora blight of pepper

A technology of Rhodopseudomonas and biocontrol agents, which is applied in the prevention and control of agricultural diseases and insect pests, in the field of Rhodopseudomonas strains and their bacterial liquid, which can solve the problems of difficult chemical control, outstanding drug resistance, pesticide residues, etc. problems, to achieve the effect of low cost, short cultivation time, and no phytotoxicity of crops

Active Publication Date: 2013-07-31
CHANGSHA AGREEN BIO TECH LTD CO
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0005] The technical problem to be solved by the present invention is to provide a simple process, green environmental protection, The easy-to-use rhodopseudomonas strains and the corresponding biocontrol agents and biocontrol fermentation liquid prepared by them, and at the same time provide the preparation method and application of the corresponding antibacterial agents and fermentation liquid, which can prevent and treat pepper blight

Method used

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  • Rhodopseudomonas strain, biocontrol microbial inoculum and biocontrol fermentation liquid, and corresponding preparation methods and application thereof in controlling phytophthora blight of pepper
  • Rhodopseudomonas strain, biocontrol microbial inoculum and biocontrol fermentation liquid, and corresponding preparation methods and application thereof in controlling phytophthora blight of pepper

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0026] Embodiment 1: Rhodopseudomonas PSB-06 biocontrol agent and preparation method thereof.

[0027] A strain of Rhodopseudomonas, named Rhodopseudomonas PSB-06, its preservation number in the China Center for Type Culture Collection is CCTCC No: M2012518, the address of the preservation unit is located in Wuhan University, China, and the preservation date is 2012 December 7th.

[0028] The above rhodopseudomonas strains can be applied in antagonizing Phytophthora capsici or preventing and controlling capsicum blight.

[0029] In this example, first use Rhodopseudomonas PSB-06 (i.e. Rhodopseudomonas CCTCC M2012518) to make a biocontrol agent. The biocontrol agent is activated and seeded by Rhodopseudomonas PSB-06 , Obtained after production and cultivation. The specific preparation method of the biocontrol agent of the present embodiment comprises the following steps:

[0030] (1) Activation: Cultivate the ultra-low temperature preservation species of Rhodopseudomonas PSB...

Embodiment 2

[0035] Embodiment 2: Rhodopseudomonas PSB-06 biocontrol fermentation liquid and its preparation method.

[0036] A biocontrol fermented liquid of the Rhodopseudomonas strain of the present invention, the biocontrol fermented liquid is mainly the supernatant obtained by fermenting and centrifuging the biocontrol bacterial agent obtained in Example 1.

[0037] The preparation method of the biocontrol fermentation liquid of the present embodiment specifically includes the following steps: take the biocontrol agent prepared in Example 1, ferment and cultivate it for 6 days, and ferment and cultivate it at a temperature of 30°C to 35°C and a light condition of 3000Lx It is carried out under the condition of ~4000Lx, the composition of the fermentation medium is the same as that of the above-mentioned photosynthetic bacteria liquid medium, and then centrifuged at 6000rpm for 5min to 8min, the supernatant after centrifugation is collected, and the supernatant after centrifugation is f...

Embodiment 3

[0038] Example 3: Inhibition effect of Rhodopseudomonas PSB-06 biocontrol fermentation liquid on Phytophthora capsici.

[0039] Mix the biocontrol fermentation broth of Example 2 with 5×PDA medium in molten state at 45°C to 50°C (200g of potato juice, 2g of glucose, 200ml of water) according to 1:2, 1:1, 2:1 respectively , to make a mixed plate; take the medium of PDA without adding the above-mentioned biocontrol fermentation liquid and the PDA medium of adding photosynthetic bacteria medium as controls, which are respectively denoted as CK and CK+P. Insert a Phytophthora capsici fungus cake with a diameter of 6mm that has been pre-cultured for 5 days in the center of the plate, inoculate one piece on each plate, repeat 3 times for each treatment, and place it upside down in a 28°C incubator for constant temperature cultivation. Record the size of the colony diameter, and calculate the bacteriostatic rate according to the following formula:

[0040] Bacterial inhibition rate ...

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Abstract

The invention discloses a Rhodopseudomonas strain. The name of the Rhodopseudomonas strain is Rhodopseudomonas PSB-06, and the collection number is CCTCC NO:M2012518. The invention also discloses a biocontrol microbial inoculum which is obtained through the activation, seed culture and production culture of the strain. The preparation of the biocontrol microbial inoculum comprises the multiple steps of activation, seed culture and production culture. The invention also discloses a Rhodopseudomonas strain biocontrol fermentation liquid which is prepared from the biocontrol microbial inoculum through fermentation and centrifugation. Each of the Rhodopseudomonas strain, the biocontrol microbial inoculum and the biocontrol fermentation liquid can be used in antagonizing phytophthora capsici or controlling phytophthora blight of pepper.

Description

technical field [0001] The invention belongs to the technical field of biopesticides, and in particular relates to a rhodopseudomonas strain and its bacterial solution, and also relates to a corresponding preparation process and application in the prevention and control of agricultural diseases and insect pests. Background technique [0002] Capsicum, also known as pepper, sea pepper, pepper, spicy horn, Qin pepper, etc., is a plant of the genus Capsicum in the family Solanaceae. Capsicum is an annual or perennial herb, and capsicum is an indispensable vegetable and condiment in people's dietary life. At present, pepper has developed into the second largest vegetable crop after Chinese cabbage in my country, and its output value and benefit then rank first among vegetable crops. [0003] Pepper production is affected by many diseases and insect pests, and pepper blight is one of the main soil-borne diseases. The disease has a wide distribution range and occurs in all peppe...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12N1/20C12P1/04A01N63/00A01N63/02A01P3/00C12R1/01
Inventor 刘勇罗源华张德咏张卓张松柏彭静谭新球成飞雪
Owner CHANGSHA AGREEN BIO TECH LTD CO
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