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Compound microorganism culture and application thereof in producing carotenoid

A technology of compound microorganisms and cultures, applied in the field of microorganisms, to achieve the effect of low raw materials and reduced enterprise costs

Active Publication Date: 2018-11-13
黑龙江省黑沃土生物科技有限公司
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0004] The purpose of the present invention is to solve the defects in the production of carotenoids in the prior art, and provide a compound microbial culture and its application in the production of carotenoids

Method used

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  • Compound microorganism culture and application thereof in producing carotenoid
  • Compound microorganism culture and application thereof in producing carotenoid

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0034] A composite microbial culture comprising Bacillus subtilis, Rhodopseudomonas capsulata and Rhodotorula viscosus;

[0035] First, the rice straw is crushed to 2 cm, and then subjected to steam explosion pretreatment under the conditions of a pressure of 1.5 MPa and a residence time of 10 min;

[0036] Pass the steam-exploded rice straw through a 50-mesh sieve, collect the under-sieve, and then mix it with distiller's grains at a mass ratio of 3:1 to obtain a mixture, then add it to water that accounts for 2 times the weight of the mixture, heat to 60°C, and keep warm. Stir at 100rpm for 90min, then heat to 121°C, keep warm for 5min, and naturally cool to room temperature to obtain a culture solution;

[0037] Put the Bacillus subtilis seed solution into the photosynthetic culture fermenter containing the culture solution according to the inoculum amount of 7% (volume ratio) for cultivation, the temperature is 30°C, the dissolved oxygen concentration is 1mg / L, the light i...

Embodiment 2

[0042] A composite microbial culture comprising Bacillus subtilis, Rhodopseudomonas capsulata and Rhodotorula viscosus;

[0043] First, the rice straw is crushed to 5 cm, and then subjected to steam explosion pretreatment under the conditions of a pressure of 1.8 MPa and a residence time of 5 min;

[0044] Pass the steam-exploded rice straw through a 50-mesh sieve, collect the under-sieve, and then mix it with distiller's grains at a mass ratio of 3:1 to obtain a mixture, then add it to water that accounts for 3 times the weight of the mixture, heat to 70°C, and keep warm. Stir at 100rpm for 80min, then heat to 121°C, keep warm for 4min, and naturally cool to room temperature to obtain a culture solution;

[0045] Put the Bacillus subtilis seed solution into the photosynthetic culture fermenter containing the culture solution according to the inoculation amount of 6% (volume ratio) for cultivation, the temperature is 30°C, the dissolved oxygen concentration is 2mg / L, the light...

Embodiment 3

[0050] The changes in the main components of rice straw caused by blasting are shown in Table 1:

[0051] Table 1

[0052] index

[0053] Conclusion: Blasting caused the cell wall of straw to be destroyed, and some hemicellulose and cellulose were degraded and dissolved, which was beneficial to the subsequent enzymatic hydrolysis of cellulose by cellulase; the crystallinity and polymerization degree of cellulose decreased during the blasting pretreatment process, and hemicellulose passed It is degraded into monosaccharides and oligosaccharides by hydrolysis, which can be used as a carbon source for the strain.

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Abstract

The invention belongs to the technical field of microorganisms and discloses a compound microorganism culture for preparing carotenoid. The compound microorganism culture comprises bacillus subtilis,rhodopseudomonas capsulate and rhodotorula glutinis. The compound microorganism culture increases yield of carotenoid and lowers culture cost.

Description

technical field [0001] The invention belongs to the technical field of microbes, and in particular relates to a compound microbial culture and its application in the production of carotenoids. Background technique [0002] Carotenoids are a kind of fat-soluble pigments, most of which are difficult to dissolve in water but soluble in organic solvents. They are yellow, orange or red polyene compounds. Because of their wide distribution, diverse structures and many functions, they have become an important of natural pigments. It exists simultaneously in plants, animals and microorganisms. Carotenoids have a variety of biological effects due to their structural properties, and studies have shown that carotenoids may have preventive and other biologically active effects on heart disease, cancer, cataracts, and several chronic human diseases. In addition, carotenoids, as an excellent food additive and nutritional supplement for improving human nutrition, have long been recognize...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12N1/20C12N1/16C12P23/00C12R1/125C12R1/38C12R1/645
CPCC12N1/16C12N1/20C12P23/00
Inventor 李玉刚钟银珊应建航应佳丽
Owner 黑龙江省黑沃土生物科技有限公司
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