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Method for cultivating photosynthetic bacteria in Rhodopseudomonas on large scale

A technology of Rhodopseudomonas and photosynthetic bacteria, applied in microorganism-based methods, biochemical equipment and methods, bacteria and other directions, can solve problems such as inability to obtain photosynthetic bacteria, non-disclosure of culture medium technology, and achieve cost Low, economical cost and time savings, easy-to-obtain results

Inactive Publication Date: 2016-07-13
FISHERY MACHINERY & INSTR RES INST CHINESE ACADEMY OF FISHERY SCI
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

When using artificially prepared culture medium for small-scale culture in the laboratory, it is necessary to add a variety of ingredients to the culture medium, especially yeast extract or beef extract rich in nutrients, in order to promote the high-density growth and reproduction of photosynthetic bacteria. And generally only photosynthetic bacteria that grow in the planktonic state can be obtained, but photosynthetic bacteria that grow in the attached state cannot be obtained
There are also some units that can cultivate photosynthetic bacteria on a large scale and sell photosynthetic bacteria products, but the culture medium or culture medium technology is generally not disclosed

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0028] Add 2.0kg of fresh water aquatic products compound feed into the glass jar with total volume of 70L in July at high temperature. The height of the glass jar is 40cm, which contains 60L of tap water. The glass jar is placed in a glass greenhouse, and there is no shade around the glass jar. Then add Rhodopseudomonas photosynthetic bacteria mother liquor (this photosynthetic bacteria mother liquor comes by online shopping, is general aquaculture on the market with high concentration Rhodopseudomonas photosynthetic bacteria liquid) in water and feed mixture, let The photosynthetic bacteria concentration in the initial water body reached 1.0×10 8 cell / L. The feed is a freshwater fish floating granular material with a crude protein content of about 28wt% and a carbohydrate content of 40wt%.

[0029] After 3 days, it can be seen that after the feed in the tank is soaked in water, part of it disperses and sinks to the bottom of the water, and part of it still floats on the wa...

Embodiment 2

[0032]Add 1.2kg of fresh water aquatic products compound feed into the glass jar with total volume of 70L in July at high temperature. The height of the glass jar is 40cm, which contains 60L of tap water. The glass jar is placed in a glass greenhouse, and there is no shade around the glass jar. Then add Rhodopseudomonas photosynthetic bacteria mother liquor in water and feed mixture, make this photosynthetic bacteria concentration reach 2.8 * 10 in the initial water body 8 cell / L. The feed is a sinking pellet with a crude protein content of about 35 wt% and a carbohydrate content of 35 wt%.

[0033] After 3 days, it could be seen that the feed in the tank was soaked in water, scattered and sank to the bottom of the water, and a faint sour smell appeared, and the water color was light yellowish white; after 7 days, there were more foams in the tank, the water body became light khaki, and the odor increased. After 15 days, the feed rot was more obvious, the smell was heavier, ...

Embodiment 3

[0036] In August of high temperature, 12.0kg of fresh water aquatic products compound feed is added to the plexiglass column that total volume is 140L, and the plexiglass column height is 100cm, and tap water 100L is filled therein. The plexiglass column is placed in the glass greenhouse, and there is no shade around the plexiglass column. Then add Rhodopseudomonas photosynthetic bacteria mother liquor in water and feed mixture, make this photosynthetic bacteria concentration reach 3.3 * 10 in the initial water body 9 cell / L. The feed is a freshwater fish compound feed with a crude protein content of about 40wt% and a carbohydrate content of 32wt%.

[0037] After 3 days, it can be seen that part of the feed in the column dispersed and sank to the bottom of the water after being soaked in water, and some still floated on the water surface, with a faint sour smell, and the water color was light yellowish white; after 7 days, more foam appeared in the column, and the water body ...

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Abstract

The invention discloses a method for cultivating photosynthetic bacteria in Rhodopseudomonas on large scale, comprising: adding common aquatic compound feed into tap water according to a ratio of compound feed to water being 20-120 g to 1L; placing in a light-permeable container in an open lit environment at 28-45 DEG C, adding photosynthetic bacteria mother liquid for fermentation culture, wherein addition density of photosynthetic bacteria is 1.0*108-5.0*1010 cell / L at beginning, cultivating until dense photosynthetic bacteria in Rhodopseudomonas growing to 1 mm in thickness attach to the inner wall of the container, and growing high-density photosynthetic bacteria in water to obtain high-density photosynthetic bacteria in Rhodopseudomonas in both attaching and floating statuses.The compound feed can be converted into photosynthetic bacteria in Rhodopseudomonas, harmless treatment and utilization of nutrients in a water body can be promoted, conversion of the nutrients in the water body can be accelerated, and the formed photosynthetic bacteria are directly useful in aquaculture or sewage purification.

Description

technical field [0001] The invention relates to a method for cultivating water body microorganisms, in particular to a method for large-scale cultivating photosynthetic bacteria of the genus Rhodopseudomonas. Background technique [0002] Photosynthetic bacteria (Photosynthetic Bacteria) is a general term for a class of prokaryotic organisms with a primitive light energy synthesis system, which can perform non-oxygenous photosynthesis under anaerobic conditions, and is a class of Gram-negative bacteria. At present, the photosynthetic bacteria species that are widely used in the fields of water treatment and aquaculture are Rhodopseudomonas (Rhodopseudomonas) of the family Rhodospirillum, which belongs to the kingdom of prokaryotes in microbial classification, and the phototrophic prokaryotic phylum. Red Photosynthetic Bacteria, Rhodospirillales. The main characteristics of photosynthetic bacteria: can withstand low temperature, will not die even if frozen; can withstand hig...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12N1/20C12R1/01
CPCC12N1/20
Inventor 王小冬金娇辉刘兴国顾兆俊车轩陆诗敏朱林时旭
Owner FISHERY MACHINERY & INSTR RES INST CHINESE ACADEMY OF FISHERY SCI
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