The invention discloses an extraction method of stevioside. The extraction method comprises the following steps: crushing dry stevia leaves, adding into an extraction tank, then adding water of whichthe mass is several times of that of the stevia, and stirring; adding cellulase, laccase and pectinase into the extraction tank, and extracting while stirring; adding a flocculant into the extractiontank, flocculating, and filtering to obtain a filtrate; adsorbing the filtrate by using macroporous adsorption resin, desorbing by using ethanol, concentrating a desorbed solution, and then performingdesalting desorption by using cationic resin to obtain a secondarily desorbed solution; concentrating the secondarily desorbed solution, and performing spray drying to obtain stevioside powder. Through addition of a mixed enzyme of the cellulase, the laccase and the pectinase into a stevia crushing solution, the addition amount of the water and the number of extractions in the extracting step arereduced, and the extracting yield is 13-32% higher than that when no enzyme is added, and the concentration cost and the operation complexity of a subsequent process are reduced.