Multi-step efficient enzyme hydrolysis process method of lignocellulose

A technology of lignocellulose and process method, which is applied in the direction of fermentation, etc., can solve the problems of increasing production cost, post-treatment process complexity, complex reactor design, large amount of cellulase, etc., to reduce concentration cost, reduce water consumption, The effect of low investment cost

Inactive Publication Date: 2017-09-29
QINGDAO INST OF BIOENERGY & BIOPROCESS TECH CHINESE ACADEMY OF SCI
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  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0005] Although cellulose hydrolysis technology has made some progress, there are still many difficult problems to solve: for example, in order to obtain a higher hydrolysis conversion rate and sugar product concentration, the amount of cellulase is usually large, and the amount of enzyme added is usually 20-45FPU / g raw material; additional additives are required to promote enzymatic hydrolysis, which increases the production cost and the complexity of the product post-treatment process; the complex reactor design leads to high investment costs
These unfavorable factors restrict the development of related industries of fiber sugar platform

Method used

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  • Multi-step efficient enzyme hydrolysis process method of lignocellulose
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Experimental program
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Effect test

Embodiment 1

[0030] The lignocellulose of wheat straw after 20wt% ammonium sulfite delignification treatment (140°C, 2 hours, liquid-to-solid ratio 6) was used as the substrate of enzymatic hydrolysis for two-stage enzymatic hydrolysis to sugar production. The specific reaction conditions of 1# and 2# enzymatic hydrolysis tanks are respectively: enzyme consumption is 11FPU / g substrate and 4FPU / g substrate (enzyme total consumption 15FPU / g substrate); Enzyme hydrolysis time is 36 hours and 12 hours ( The total reaction time is 48 hours); the temperature, substrate concentration and pH value of each stage of enzymatic hydrolysis are 50° C., 8% and 4.8. When the sixth batch of enzymatic hydrolysis of lignocellulosic raw materials was carried out, the enzymatic hydrolysis system reached a stable production state. At this time, the sugar concentration obtained by the 1# enzyme hydrolysis tank is about 53g / L, and the total sugar conversion rate of the substrate is 82%.

[0031] The shape change...

Embodiment 2

[0033]Using commercial bleached softwood pulp as raw material (enzymatic hydrolysis substrate), directly adopt 3-stage enzymatic hydrolysis to produce sugar. The specific reaction conditions of 1#, 2# and 3# enzyme hydrolysis tanks are: enzyme dosage 20FPU / g substrate, 5FPU / g substrate and 5FPU / g substrate (total enzyme dosage 30FPU / g substrate); enzyme hydrolysis The time is 48 hours, 12 hours and 12 hours (the total reaction time is 72 hours); the substrate concentration is 20%, 15% and 10%; the temperature and pH value of each stage of enzymatic hydrolysis are 50°C and 4.8. When the enzymatic hydrolysis of the eighth batch of lignocellulosic raw materials was carried out, the enzymatic hydrolysis system reached a stable production state. At this time, the sugar concentration obtained by the 1# enzyme hydrolysis tank is about 126g / L, and the total sugar conversion rate of the substrate is 77%.

Embodiment 3

[0035] After corn stalks were delignified with 12wt% NaOH (130° C., 2 hours, liquid-solid ratio 6), three stages of enzymatic hydrolysis for sugar production were carried out. The specific reaction conditions of 1#, 2# and 3# enzyme hydrolysis tanks are respectively: the enzyme dosage is 12FPU / g substrate, 5FPU / g substrate and 3FPU / g substrate (the total enzyme dosage is 20FPU / g substrate); The hydrolysis time is 24 hours, 12 hours and 12 hours (the total reaction time is 48 hours); the temperature, substrate concentration and pH value of each stage of enzyme hydrolysis are 50° C., 12% and 4.8. When the enzymatic hydrolysis of the eighth batch of lignocellulosic raw materials was carried out, the enzymatic hydrolysis system reached a stable production state. At this time, the sugar concentration of the product obtained by the 1# enzyme hydrolysis tank is about 82g / L, and the conversion rate of the total sugar of the substrate is 83%.

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Abstract

The invention discloses a multi-step efficient enzyme hydrolysis process method of lignocellulose. The method comprises the following steps of 1, feeding a fresh lignocellulose raw material, cellulase and water into a first enzyme hydrolysis tank, and performing hydrolysis under a condition of certain temperature and pH value; 2, after the first enzyme hydrolysis reaction in the step 1 is finished, performing solid-liquid separation, wherein the liquid obtained through separation is directly taken out to be used as a sugar product, and the solid obtained through separation is directly fed into a second enzyme hydrolysis tank to be reacted; 3, after the second enzyme hydrolysis reaction in the step 2 is finished, performing solid-liquid separation, wherein the liquid obtained through separation is conveyed into the first enzyme hydrolysis tank to continuously participate in the enzyme hydrolysis reaction, and the solid obtained through separation is directly fed into a third enzyme hydrolysis tank to be continuously reacted; etc.and n, after the (n-1)th enzyme hydrolysis reaction in the step (n-1) is finished, performing solid-liquid separation, wherein the liquid obtained through separation is conveyed into the (n-2)th enzyme hydrolysis tank to continuously participate in the enzyme hydrolysis reaction, and the solid obtained through separation is directly fed into an nth enzyme hydrolysis tank to be continuously reacted.

Description

technical field [0001] The invention belongs to the technical field of lignocellulosic enzymatic hydrolysis, and in particular relates to a method for improving lignocellulosic enzymatic hydrolysis saccharification conversion rate and product sugar concentration by using a multi-stage enzymatic hydrolysis process. Background technique [0002] With the shortage of petroleum resources and people's increasing attention to energy and environmental issues, the utilization of lignocellulosic biomass has been paid more and more attention by countries all over the world, and has become a new development field and scientific research hotspot. Lignocellulose is an abundant and cheap renewable resource, and the dry matter produced by photosynthesis of green plants around the world reaches 1.55×10 11 tons, mainly including cellulose, hemicellulose and lignin. [0003] Efficient hydrolysis of lignocellulose into fermentable sugars is a crucial link in the conversion process of biofuels...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12P19/14
CPCC12P19/14
Inventor 刘超李滨冯晓燕
Owner QINGDAO INST OF BIOENERGY & BIOPROCESS TECH CHINESE ACADEMY OF SCI
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