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Extraction method of stevioside

A steviol glycoside and extraction technology, applied in the field of biological extraction, can solve the problems of limited outflow of active ingredients, long time, low outflow rate, etc., and achieve the effects of reducing difficulty, reducing the amount of added water, and reducing the number of extractions.

Inactive Publication Date: 2018-10-16
HUAIYIN TEACHERS COLLEGE
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Because the plant cell wall structure is complex, the outflow of active ingredients is restricted, resulting in long time and low outflow rate

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0022] Example 1: Weigh 100 kg of stevia dry leaves, mechanically crush them to a size of 5-10 cm, and put them into an extraction tank; add 500 L of tap water, add 5 g of cellulase at 200 U / g (the ratio is 0.01 U / g), 10,000 U 0.5g of laccase / g (the ratio is 0.05U / g), 16.5g of 30,000 U / g pectinase (the ratio is 5U / g), leaching and stirring for 45min, the leaching temperature is 37°C, and steviol glycosides are obtained. Extract liquid; add sodium polyacrylate to the extraction tank, flocculate for 30 minutes, and filter to obtain 488L of filtrate; after the obtained filtrate is absorbed by D107 macroporous adsorption resin, it is analyzed with ethanol with a mass concentration of 75%, and the analysis liquid is concentrated 3 times After desalination and analysis with 732 cationic resin, the secondary analysis solution was obtained; after the secondary analysis solution was concentrated to 80L, it was spray-dried to obtain 11.6kg of finished steviol glycoside powder.

[0023] ...

Embodiment 2

[0024] Example 2: Weigh 100 kg of stevia dry leaves, mechanically crush them to a size of 5-10 cm, and put them into an extraction tank; add 300 L of tap water, add 200 U / g of cellulase 50 g (the ratio is 0.1 U / g), 10,000 U 5g of laccase / g (the ratio is 0.5U / g), 66g of 30,000 U / g pectinase (the ratio is 20U / g), leaching and stirring for 30min, the leaching temperature is 37°C, and the steviol glycoside extract is obtained Add sodium polyacrylate in the extraction tank, after flocculation for 45min, filter to obtain 290L of filtrate; after the obtained filtrate is adsorbed by D107 type macroporous adsorption resin, analyze with ethanol with a mass concentration of 75%, and analyze the solution after being concentrated 3 times. 732 cationic resin was desalted and analyzed to obtain a secondary analysis solution; after the secondary analysis solution was concentrated to 60L, it was spray-dried to obtain 11.8kg of finished steviol glycoside powder.

[0025] Among them, in the fini...

Embodiment 3

[0026] Example 3: Weigh 100 kg of stevia dry leaves, mechanically crush them to a size of 5-10 cm, and put them into an extraction tank; add 400 L of tap water, add 25 g of 200 U / g cellulase (the ratio is 0.05 U / g), 10,000 U 2g of laccase / g (ratio of 0.2U / g), 33g of pectinase of 30,000 U / g (ratio of 10U / g), leaching and stirring for 40min, leaching temperature of 37°C, to obtain steviol glycoside extract Add sodium polyacrylate to the extraction tank, after flocculation for 40min, filter to obtain 388L of filtrate; after the obtained filtrate is adsorbed by D107 type macroporous adsorption resin, analyze with ethanol with a mass concentration of 75%, and the solution is concentrated by 732 The cationic resin was desalted and analyzed to obtain a secondary analysis solution; after the secondary analysis solution was concentrated to 70L, it was spray-dried to obtain 11.8kg of steviol glycoside powder finished product.

[0027] Among them, in the finished product of steviol glyco...

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PUM

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Abstract

The invention discloses an extraction method of stevioside. The extraction method comprises the following steps: crushing dry stevia leaves, adding into an extraction tank, then adding water of whichthe mass is several times of that of the stevia, and stirring; adding cellulase, laccase and pectinase into the extraction tank, and extracting while stirring; adding a flocculant into the extractiontank, flocculating, and filtering to obtain a filtrate; adsorbing the filtrate by using macroporous adsorption resin, desorbing by using ethanol, concentrating a desorbed solution, and then performingdesalting desorption by using cationic resin to obtain a secondarily desorbed solution; concentrating the secondarily desorbed solution, and performing spray drying to obtain stevioside powder. Through addition of a mixed enzyme of the cellulase, the laccase and the pectinase into a stevia crushing solution, the addition amount of the water and the number of extractions in the extracting step arereduced, and the extracting yield is 13-32% higher than that when no enzyme is added, and the concentration cost and the operation complexity of a subsequent process are reduced.

Description

technical field [0001] The invention belongs to the field of biological extraction, and specifically relates to a process for extracting steviol glycosides from stevia rebaudiana, in which cellulase, laccase and pectinase are added during the extraction process to improve the extraction yield and efficiency Methods. Background technique [0002] Steviol Glycosides, also known as steviol glycosides and steviosides, is a high-sweetness, low-calorie natural non-nutritive sweetener extracted from the leaves of Compositae plants (Stevia Rebaudia). Steviol glycosides have multiple pharmacological activities in the body and have no toxic side effects, and have been used as a substitute for natural sucrose. Steviol glycosides mainly include stevioside, steviobioside and rebaudioside A-F, among which stevioside and steviobioside account for the main content. Steviol glycosides are extracted, clarified and crystallized from the dried leaves of Stevia rebaudiana with water. In actual...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C07H15/256C07H1/08
CPCC07H1/08C07H15/256
Inventor 贺建龙熊鹏刘晓燕夏军徐继明
Owner HUAIYIN TEACHERS COLLEGE
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