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Application of photosynthetic bacterium and bacillus subtilis mixed bacterial liquid in preparing microbial inoculum for preventing and controlling red mouth disease of turbot

A technology of Bacillus subtilis and photosynthetic bacteria, which is applied in the biological field to achieve the effects of effective bacterial agents, no pathogenic bacteria resistance, and good safety

Inactive Publication Date: 2010-12-22
SOUTH CHINA UNIV OF TECH
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0006] The purpose of the present invention is to overcome the deficiencies of various food safety problems caused by the abuse of antibiotics in the existing treatment of fish diseases, and to provide an application of a mixed bacterial solution of photosynthetic bacteria and Bacillus subtilis in the preparation of a bacterial agent for preventing and treating turbot redmouth disease

Method used

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  • Application of photosynthetic bacterium and bacillus subtilis mixed bacterial liquid in preparing microbial inoculum for preventing and controlling red mouth disease of turbot
  • Application of photosynthetic bacterium and bacillus subtilis mixed bacterial liquid in preparing microbial inoculum for preventing and controlling red mouth disease of turbot
  • Application of photosynthetic bacterium and bacillus subtilis mixed bacterial liquid in preparing microbial inoculum for preventing and controlling red mouth disease of turbot

Examples

Experimental program
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Effect test

Embodiment 1

[0020] The preparation of embodiment 1 fungicide

[0021] (1) Preparation of Photosynthetic Bacteria Liquid

[0022] Concentration is 10 9 cfu / mL of Rhodopseudomonas sp. (Rhodopseudomonas sp., CGMCC 1.2193, purchased from China General Microorganism Culture Collection and Management Center) was inoculated into the optimized RCVBN liquid medium at 0.1% of the medium volume, at 30°C, light intensity The bacterium solution obtained after 48h of light anaerobic cultivation under 3000lx was inoculated into the optimized RCVBN medium according to 10% of the medium volume, and light anaerobic fermentation was cultivated for 48h, and then 6000rpm was centrifuged for 15min to obtain the thalline, and the thalline was added without Bacteria in phosphate buffer can be obtained at a concentration of 10 12 cfu / mL photosynthetic bacteria broth.

[0023] (2) Preparation of Bacillus subtilis bacterial liquid

[0024] Concentration is 10 9 Cfu / mL Bacillus subtilis (Bacillus Subtilis Cohn,...

Embodiment 2

[0026] Application of embodiment 2 fungicides 1

[0027] There are 5000 turbots in each turbot breeding pond (the turbot comes from a fish farm in Shandong Province), the average weight of the turbot is 25±0.5g, and the water temperature is 25°C.

[0028] There are four experimental groups A, B, C, and D on average, and each experimental group of B, C, and D has 4 groups, namely B1, C1, D1; B2, C2, D2; B3, C3, D3; B4, C4, D4; 2 repetitions in each group. The five experimental groups were treated as follows:

[0029] Group A: the breeding method of factory production (control group).

[0030] Group B: Sprinkle the mixed bacterial solution of photosynthetic bacteria and Bacillus subtilis in the water, so that the concentration of the mixed bacterial solution of photosynthetic bacteria and Bacillus subtilis in the water body reaches 10 and 10 respectively. 3 、10 5 、10 7 cfu / mL, fed common feed, and added bacteria after changing the whole pool water every 40 days.

[0031] G...

Embodiment 3

[0043] Example 3 Application of fungicides 2

[0044] Each turbot breeding pond suffers from 30 turbots with red mouth disease (sick fish snout and ventral maxillary hemorrhage), the water temperature is 25°C, and the treatment time is 8 days.

[0045]There are five experimental groups A, B, C, D, and E on average, and each experimental group C, D, and E has 4 groups, namely C1, D1, E1; C2, D2, E2; C3, D3, E3; C4, D4, E4; C1, E1; C2, E2; C3, E3; C4, E4 each group is divided into 2 groups, namely C11, C12...E41, E42, each group has 2 repetitions. The five experimental groups were treated as follows:

[0046] Group A: the breeding method of factory production (control group).

[0047] Group B: splash antibiotics (povidone-iodine, 1mg / L-2mg / L) in water, and feed common feed.

[0048] Group C: Sprinkle the mixed bacterial solution of photosynthetic bacteria and Bacillus subtilis in the water, so that the concentration of the mixed bacterial solution of photosynthetic bacteria a...

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Abstract

The invention discloses an application of photosynthetic bacterium and bacillus subtilis mixed bacterial liquid in preparing microbial inoculum for preventing and controlling red mouth disease of turbot. The application is characterized in that photosynthetic bacterium culture medium and bacillus subtilis culture medium are mixed according to the ratio of 1:1 on the basis of the number of bacteria; the photosynthetic bacterium is at least one of rhodopseudomonas, rhodopseudomonas palustris and capsule erythrocyte. The application method of the bacterial liquid comprises the following steps: spraying bactericide into a water body to ensure that the final concentration of the photosynthetic bacterium and bacillus subtilis mixed bacterial liquid is between 10 and 107cfu / ml, or dipping feed in the photosynthetic bacterium and bacillus subtilis mixed bacterial liquid, wherein the concentration of the photosynthetic bacterium and the bacillus subtilis in the mixed bacterial liquid is between 10 and 107cfu / mL. The bactericide is effective, environement-friendly and without public nuisance, has excellent application prospect, and has important guidance meaning for preventing overuse of antibiotics.

Description

technical field [0001] The invention belongs to the field of biotechnology, and relates to the application of fungi, in particular to the application of photosynthetic bacteria and bacillus subtilis in preparing fungal agents for preventing and treating turbot redmouth disease. Background technique [0002] Turbot is a rare flounder native to the coast of Europe. Its body is flat and nearly round. The eyes are located on the left side of the body. The side with the eyes is blue-brown, with some melanin and a small amount of prickles, and the side without eyes is smooth and white. The dorsal and anal fins have no hard spines, are very long, the head and tail fins are small, the middle part of the body is thick, and the internal organs are small. Turbot is naturally distributed along the European coast on the eastern side of the Atlantic Ocean, and is commonly found in the North Sea and Baltic Sea. It has the advantages of adapting to low water temperature life, fast growth, ...

Claims

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Application Information

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IPC IPC(8): A01N63/00A01P1/00A61K35/74A61P31/04A61K35/742
Inventor 蔡俊鹏肖小丽
Owner SOUTH CHINA UNIV OF TECH
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