Preparation method of high-concentration microalgae living cell long-acting preservative

Abstract

The invention discloses a preparation method of a high-concentration microalgae living cell long-acting preservative. The preparation method comprises the steps of fermenting and culturing rhodopseudomonas capsulate and halophila campisalis in a glutamate culture medium to obtain bacterial sludge, embedding the bacterial sludge into sodium alginate carrier particles, and continuously adsorbing nutrient matrix powder containing inorganic salts, vitamins, organic carbon sources and the like into the carrier particles to obtain photosynthetic bacterium carrier particle nutrient salt mixed particles, and carrying out spray drying and powder homogenization to obtain the high-concentration microalgae living cell long-acting preservative. The preservative is simple to use, only a certain amount of preservative powder needs to be added into the concentrated algae liquid, bacteria are increased in the embedded particles, and the generated metabolites can maintain the ecological balance of an algae concentrated solution. The method is simple and feasible, and the vigor of concentrated algae living cells can be maintained for 10-30 days in spring, summer, autumn and autumn without decay.

Description

technical field [0001] The invention relates to a method for maintaining and improving cell viability during long-term storage and transportation of a large amount of microalgae concentrate in hot season. Background technique [0002] Aquatic microalgae such as Chlorella, Dunaliella salina, Haematococcus pluvialis, etc., because microalgae themselves contain a lot of nutrients and pigment content, are the best aquatic food. At present, there are mainly microalgae living cells and microalgae dry powder. Microalgae living cells are rich in nutrients, but are prone to death and spoilage during transportation. The existing microalgae concentrates are usually transported by low-temperature refrigeration, which leads to problems such as high cost and low algal cell viability. [0003] At present, the transportation of bait algae mainly includes the following methods. [0004] (1) Low temperature transport of microalgae, low temperature usually refers to the temperature of 0-4 °...

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Problems solved by technology

[0004] (1) Microalgae are transported at low temperature. Low temperature usually refers to the temperature at 0-4°C. At this temperature, this species can transport 2×10 microalgae per ml. 10 Cell concentration, there is a problem of high cost, and the vigor of algae is significantly reduced, so it is not suitable for the situation where live algae regulate the water quality of aquaculture water

[0005] (2) Microalgae are transported at room temperature. Microalgae are transported at room temperature in two ways: light and dark. However, under light conditions, the concentration of algae cells is low, generally 10 per ml. 6 less than 10 cells per milliliter 7 The algae cell concentrate will be spoiled within 24 hours, the transportation efficiency is low, and the cost is high

[0011] 3. Patent (200410036386.8) "Preparation method of dry products of Monocystis algae and its products and applications". This method is convenient for transportation by concentrating, drying, vacuuming or nitrogen-filling the algae, but the algae cells in this patent have died, and the algae partial loss of nutrients in cells

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