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Culture medium of rhodopseudomonas palustris capable of efficiently reducing COD of water

A technology of rhodopseudomonas palustris and culture medium, which is applied in the field of culture medium for efficiently reducing COD of rhodopseudomonas palustris in water bodies, can solve the problems of increasing the cost of breeding, doubling the amount of use, and difficult to separate, so as to reduce the cost of breeding, The effect of reducing the amount of use and improving the breeding environment

Active Publication Date: 2016-05-04
GUANGZHOU UNIVERSITY +1
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  • Abstract
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  • Claims
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Problems solved by technology

At present, the conventional medium plate separation method is used for isolation and culture. Due to the presence of oxygen pressure and the difficulty of separating high-efficiency Rhodopseudomonas palustris with strong COD degradation ability in ordinary medium, the strains obtained are often not strong in degrading organic matter in aquaculture ponds. , leading to double the use of Rhodopseudomonas palustris in the culture pond, increasing the cost of cultivation, and becoming a bottleneck restricting the production of Rhodopseudomonas palustris

Method used

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  • Culture medium of rhodopseudomonas palustris capable of efficiently reducing COD of water
  • Culture medium of rhodopseudomonas palustris capable of efficiently reducing COD of water
  • Culture medium of rhodopseudomonas palustris capable of efficiently reducing COD of water

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Experimental program
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Embodiment 1

[0016] Enrichment medium: NH 4 SO 4 1g, MgCl 2 0.2g, NaHCO 3 5g,K 2 HPO 4 0.5g, 2g NaCl, 0.8g yeast extract, 40ml trace element biopromoting mother solution, 1 liter water, pH7.1.

[0017] Separation medium: NH 4 SO 4 1g, MgCl 2 0.2g, sodium acetate 3g, NaHCO 3 2.8g, K 2 HPO 4 0.5g, 1.9g of NaCl, 0.4g of yeast extract, 19ml of trace element biopromoting mother solution, 1 liter of water, pH7.1; 19 grams of agar was added to the formula of the separation medium.

[0018] Among them, trace elements promote biological mother solution: Na 2 EDTA500mg, FeSO 4 .7H 2 O200mg, ZnSO 4 .7H 2 O10mg, MnSO 4 .4H 2 O2mg, H 3 BO 3 50mg, CoCl 2 .2H 2 O20mg, NiCl 2 .6H 2 O7mg, CuCl 2 .6H 2 O0.5mg, Na 2 MoO 4 .2H 2 O4mg, CaCl 2 200mg, all ingredients were dissolved in 1000ml water, pH4.4, filter sterilized.

[0019] Isolation of Rhodopseudomonas palustris using the above medium:

[0020] S1. Enrichment culture of Rhodopseudomonas palustris: Put 20g of the black bot...

Embodiment 2

[0023] Enrichment medium: NH 4 SO 4 1.1g, MgCl 2 0.3g, NaHCO 3 5.1g, K 2 HPO 4 0.6g, NaCl2.1g, yeast extract 0.9g, trace element biopromoting mother liquid 41ml, water 1 liter, pH7.2.

[0024] Separation medium: NH 4 SO 4 1.1g, MgCl 2 0.3g, sodium acetate 3.1g, NaHCO 3 2.9g, K 2 HPO 4 0.6g, 2.0g NaCl, 0.5g yeast extract, 20ml trace element biopromoting mother solution, 1 liter water, pH7.23; add 20g agar to the formula of separation medium.

[0025] Among them, trace elements promote biological mother solution: Na 2 EDTA501mg, FeSO 4 .7H 2 O201mg, ZnSO 4 .7H 2 O11mg, MnSO 4 .4H 2 O2.1 mg, H 3 BO 3 51 mg, CoCl 2 .2H 2 O21mg, NiCl 2 .6H 2O7.13mg, CuCl 2 .6H 2 O0.68mg, Na 2 MoO 4 .2H 2 O4.13mg, CaCl 2 201mg, all ingredients were dissolved in 1000ml water, pH4.5, filter sterilized.

[0026] Isolation of Rhodopseudomonas palustris using the above medium:

[0027] S1. Enrichment culture of Rhodopseudomonas palustris: Put 21g of the black bottom mud of ...

Embodiment 3

[0030] Enrichment medium: NH 4 SO 4 1.45g, MgCl 2 0.35g, NaHCO 3 5.25g, K 2 HPO 4 0.7g, NaCl2.2g, yeast extract 1.0g, trace element biopromoting mother liquid 42ml, water 1 liter, pH7.21.

[0031] Separation medium: NH 4 SO 4 1.2g, MgCl 2 0.4g, sodium acetate 3.2g, NaHCO 3 2.9g, K 2 HPO 4 0.7g, NaCl2.1g, yeast extract 0.6g, trace element biopromoting mother liquid 21ml, water 1 liter, pH7.25; add 21 grams of agar to the formula of the separation medium.

[0032] Among them, trace elements promote biological mother solution: Na 2 EDTA502mg, FeSO 4 .7H 2 O202mg, ZnSO 4 .7H 2 O12mg, MnSO 4 .4H 2 O2.2mg, H 3 BO 3 52 mg, CoCl 2 .2H 2 O22mg, NiCl 2 .6H 2 O7.23mg, CuCl 2 .6H 2 O0.78mg, Na 2 MoO 4 .2H 2 O4.13mg, CaCl 2 202.3mg, all ingredients were dissolved in 1000ml of water, pH4.61, filter sterilized.

[0033] Isolation of Rhodopseudomonas palustris using the above medium:

[0034] S1. Enrichment culture of Rhodopseudomonas palustris: Put 22g of the b...

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Abstract

The invention belongs to the technical field of microorganisms, and particularly discloses a culture medium of rhodopseudomonas palustris. The micro-element biological promoting mother liquor which is prepared from 500-503 mg of Na2EDTA, 200-203 mg of FeSO4.7H2O, 10-13 mg of ZnSO4.7H2O, 2-2.3 mg of MnSO4.4H2O, 50-53 mg of H3BO3, 20-23 mg of CoCl2.2H2O, 7-7.3 mg of NiCl2.6H2O, 0.5-0.8 mg of CuCl2.6H2O, 4-4.3 mg of Na2MoO4.2H2O, 200-203 mg of CaCl2 and 1 L of water is added into the culture medium of the rhodopseudomonas palustris, wherein the pH of the micro-element biological promoting mother liquor ranges from 4.4 to 4.7. According to the culture medium of the rhodopseudomonas palustris, the special micro-element biological promoting mother liquor is utilized, the rhodopseudomonas palustris capable of efficiently reducing the chemical oxygen demand (COD) of water is obtained, and therefore the purposes of decreasing the use area of breeding ponds, improving the breeding environment and reducing the breeding cost are achieved. By means of the action of the strains separated from the culture medium, the decreasing rate of the COD in the water can reach 70.41%.

Description

technical field [0001] The invention belongs to the technical field of microbes, and in particular relates to a medium for efficiently reducing COD of water body Rhodopseudomonas palustris. Background technique [0002] At present, my country's aquaculture industry is developing rapidly, but due to improper long-term breeding methods, ponds are aging, water quality is deteriorating, organic matter is seriously polluted, COD (chemical oxygen demand) is greatly increased, and pathogenic microorganisms multiply, forming a large number of fish and shrimp diseases. Therefore seeking a kind of novel water quality improving agent has been the task of top priority. [0003] Rhodopseudomonas palustris is a microorganism that widely exists in nature. It is a kind of organism that uses light as energy, uses organic matter and sulfide in nature as nutrients, and can perform photosynthesis. It can not only purify water quality, but also contains rich nutrition, which can be used as a ba...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12N1/20C12N1/02C02F3/34C12R1/01
CPCC02F3/34C12N1/02C12N1/20
Inventor 江学斌张玲华胡位荣马苗鹏黄瀚威
Owner GUANGZHOU UNIVERSITY
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