Looking for breakthrough ideas for innovation challenges? Try Patsnap Eureka!

Microbial preparation used for repairing heavy metal contaminated soil and preparation method and application thereof

A technology of microbial preparations and composite microbial preparations, applied in the field of soil remediation, can solve problems such as poor remediation effect, easy inactivation, and high processing costs, and achieve the effect of being conducive to popularization and utilization and outstanding effects

Inactive Publication Date: 2018-10-26
江苏世邦生物工程科技有限公司
View PDF6 Cites 5 Cited by
  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Moreover, soil heavy metal pollution has the characteristics of concealment, accumulation, hysteresis and long-term nature. It is difficult to repair and the repair effect is not good. Therefore, heavy metal pollution is the focus of environmental science and technology research.
[0004] Biological enzyme is a kind of biological chelating agent, which is currently widely used to deal with the restoration of environmental pollution. When biological enzymes combine with heavy metals, they will form complexes, thereby reducing pollution, and the repair effect is better. However, the use of biological enzymes will be affected by temperature. , PH, etc., it is very easy to inactivate, which leads to a series of problems such as poor treatment effect and high treatment cost. It is urgent to find a solution that can comprehensively solve the above pollution problems.

Method used

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
View more

Image

Smart Image Click on the blue labels to locate them in the text.
Viewing Examples
Smart Image
  • Microbial preparation used for repairing heavy metal contaminated soil and preparation method and application thereof

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0026] Immobilization of high-temperature-resistant α-amylase: Add mesoporous molecular sieve material SBA-15 to high-temperature-resistant α-amylase at a temperature of 60°C; pH of 6.0; rotational speed of 220rpm; immobilization time of 16h. Obtained after transformation.

[0027] The preparation method of Rhodopseudomonas palustris DSM5859 inoculant comprises: culturing Rhodopseudomonas palustris DSM5859 to the logarithmic phase, and then inoculating the strains cultivated to the logarithmic phase to the culture medium according to the inoculation amount of 3% of the volume of the culture medium. In the base, 30 DEG C shaking table shakes 180rpm, cultivates for 72 hours, obtains the fermented liquid of Rhodopseudomonas palustris DSM5859, adds adsorbent to this fermented liquid, and dries to obtain Rhodopseudomonas palustris DSM5859 bacterial agent; The adsorbent is bentonite. The live bacteria content of Rhodopseudomonas palustris DSM5859 was 1×10 8 cfu / g.

[0028] The me...

Embodiment 2

[0035]All culture mediums adopted in this example are the same as Example 1.

[0036] Immobilization of high-temperature-resistant α-amylase: Add mesoporous molecular sieve material SBA-15 to high-temperature-resistant α-amylase at a temperature of 50°C; pH of 7.0; rotational speed of 180rpm; immobilization time of 10h. Obtained after transformation.

[0037] The preparation method of Rhodopseudomonas palustris DSM5859 inoculant comprises: culturing Rhodopseudomonas palustris DSM5859 to the logarithmic phase, and then inoculating the strains cultivated to the logarithmic phase to the culture medium according to the inoculum amount of 6% of the volume of the culture medium. In the base, 35 DEG C shaking table shakes 220rpm, cultivates for 48 hours, obtains the fermented liquid of Rhodopseudomonas palustris DSM5859, adds adsorbent to this fermented liquid, and dries, obtains Rhodopseudomonas palustris DSM5859 bacterial agent; The adsorbent is activated carbon. The live bacteri...

Embodiment 3

[0042] The culture medium in this example is the same as Example 1.

[0043] Immobilization of high temperature-resistant α-amylase: Add mesoporous molecular sieve material SBA-15 to high-temperature resistant α-amylase at a temperature of 70°C; pH of 6.0; rotation speed of 200rpm; immobilization time of 13h. Obtained after transformation.

[0044] The preparation method of Rhodopseudomonas palustris DSM5859 inoculant comprises: culturing Rhodopseudomonas palustris DSM5859 to the logarithmic phase, and then inoculating the strains cultivated to the logarithmic phase to the culture medium according to the inoculum amount of 4% of the medium volume. medium, at 32°C, shaking on a shaking table at 180rpm, and cultivating for 60 hours to obtain a fermented liquid of Rhodopseudomonas palustris DSM5859, adding an adsorbent to the fermented liquid, and drying to obtain the fermented liquid of Rhodopseudomonas palustris DSM5859 ; The adsorbent is bentonite and activated carbon. The l...

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to View More

PUM

No PUM Login to View More

Abstract

The invention discloses a microbial compound preparation used for repairing heavy metal contaminated soil. The compound microbial preparation is characterized by comprising rhodopseudomonas palustrisDSM5859 fungicide, lactobacillus plantarum NJ2205 fungicide, bacillus subtilis BNCC188062 fungicide and immobilized high temperature resistance alpha-amylase. The invention further discloses a preparation method and application of the microbial preparation. According to the microbial preparation, the immobilized high temperature resistance alpha-amylase is applied to the field of soil remediationfor the first time, the rhodopseudomonas palustris DSM5859 fungicide, the lactobacillus plantarum NJ2205 fungicide, the bacillus subtilis BNCC188062 fungicide and the immobilized high temperature resistance alpha-amylase are mixed for preparing the microbial enzyme preparation for the first time, the removal rate of Cd, Cr, Cu, Ni and Zn reaches 85% or above, and the preparation is environmentallyfriendly, cannot cause secondary pollution, and is beneficial for large-scale utilization and popularization for oil.

Description

[0001] Technical solutions [0002] The invention belongs to the technical field of soil remediation, and in particular relates to a microbial preparation for remediating heavy metal-contaminated soil, a preparation method and application thereof. Background technique [0003] With the acceleration of urbanization and industrialization, the degree of heavy metal pollution in the soil near cities and industrial areas is also increasing, and the polluted area is gradually expanding. According to reports, the heavy metal-contaminated soil in my country has exceeded 20 million hm 2 . Moreover, soil heavy metal pollution has the characteristics of concealment, accumulation, lag and long-term nature. It is difficult to repair and the repair effect is not good. Therefore, heavy metal pollution is the focus of environmental science and technology research. [0004] Biological enzyme is a kind of biological chelating agent, which is currently widely used to deal with the restoration o...

Claims

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to View More

Application Information

Patent Timeline
no application Login to View More
IPC IPC(8): C12N1/20B09C1/10C12R1/38C12R1/25C12R1/125
CPCB09C1/10C12N1/20
Inventor 谢悦波陈求稳何庆成金波姚竣耀
Owner 江苏世邦生物工程科技有限公司
Who we serve
  • R&D Engineer
  • R&D Manager
  • IP Professional
Why Patsnap Eureka
  • Industry Leading Data Capabilities
  • Powerful AI technology
  • Patent DNA Extraction
Social media
Patsnap Eureka Blog
Learn More
PatSnap group products

Browse by: Latest US Patents, China's latest patents, Technical Efficacy Thesaurus, Application Domain, Technology Topic, Popular Technical Reports.

© 2024 PatSnap. All rights reserved.Legal|Privacy policy|Modern Slavery Act Transparency Statement|Sitemap|About US| Contact US: help@patsnap.com