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Method for preparing rhodopseudomonas palustris for feed by utilizing white maize meal as raw material

A technology for swamp rhodopseudomonas and rice noodles, which is applied in the directions of microorganism-based methods, biochemical equipment and methods, bacteria, etc., can solve the problems of difficult to effectively popularize and apply, cumbersome operation process, long production cycle, etc., and achieve economic and social benefits. Remarkable, good application effect, low price effect

Active Publication Date: 2015-01-21
HENAN ACAD OF SCI INST OF BIOLOGY LIABILITY
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  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

Domestic production of Rhodopseudomonas palustris generally uses plastic barrels, etc., using the characteristics of Rhodopseudomonas paluscans to grow under light anaerobic conditions. The production cycle is long, generally more than 7 days. The combination of ingredients in the base formula is relatively complicated, and generally requires several or more than a dozen nutrients such as carbon sources, nitrogen sources, phosphorus sources, trace elements, vitamins, etc. to adjust the pH, and the actual preparation process is cumbersome. To meet the needs of the feed farming industry for feed production, its innovation is imperative

Method used

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  • Method for preparing rhodopseudomonas palustris for feed by utilizing white maize meal as raw material
  • Method for preparing rhodopseudomonas palustris for feed by utilizing white maize meal as raw material
  • Method for preparing rhodopseudomonas palustris for feed by utilizing white maize meal as raw material

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Embodiment Construction

[0023] The specific implementation of the present invention will be described in detail below in conjunction with the accompanying drawings and examples.

[0024] Given by accompanying drawing, the present invention is realized by the following steps in concrete implementation:

[0025] A. prepare bacterial classification, the method is:

[0026] (1) Strain activation, using Rhodopseudomonas palustris AS1.2352, inserting Rhodopseudomonas palustris AS1.2352 bacterial strain into the ATYP agar liquid medium in the glass petri dish, culture medium Drop sterilized liquid paraffin on the surface to seal the liquid surface, and at 29°C, irradiate with a 40W incandescent lamp at a distance of 20cm from the lower end of the lamp, and anaerobic static culture for 6 days to obtain activated strains; the ATYP agar liquid The formula of the medium is dipotassium hydrogen phosphate K 2 HPO 4 1.0 g, calcium chloride CaCl 2 0.1g, sodium bicarbonate NaHCO 3 3.0 g, sodium acetate CH 3...

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Abstract

The invention discloses a method for preparing rhodopseudomonas palustris for feed by utilizing white maize meal as a raw material. The preparation of rhodopseudomonas palustris for feed can be effectively solved, so that the requirement of the feed breeding industry for feed production is met. According to the technical scheme, the method comprises the following steps: preparing a strain, culturing the strain, preparing a liquid fermentation culture medium, inoculating, performing fermentation cultivation, performing carrier adsorption and drying. The method disclosed by the invention has the advantages that 1, the raw materials of the fermentation medium mainly are white maize meal and other materials which are rich in resources, low in price and simple and readily available; 2, the fermentation medium is simple in formula, fermentation production in an automatic liquid fermentation tank is realized, the operation is convenient, the culture is convenient, the growth speed is high, and the fermentation period is greatly shortened and is only 72-96 hours; 3, the rhodopseudomonas palustris for feed can be prepared; 4, the rhodopseudomonas palustris for feed is good in application effect and outstanding in economic and social benefits; and 5, the microbial strain rhodopseudomonas palustris AS1.2352 permitted in the Department of Agriculture in China is used for fermentation production, the safety is high, and the rhodopseudomonas palustris is a green additive for feed.

Description

technical field [0001] The invention relates to the technical field of biological fermentation, in particular to a method for preparing rhodopseudomonas palustris for feed by using white cornmeal as a raw material. Background technique [0002] According to Dong Xiuzhu's (2001) "Common Bacteria System Identification Manual" classification, Rhodopseudomonas palustris belongs to purple non-sulfur bacteria, Rhodopseudomonas genus, in the natural carbon, nitrogen, sulfur conversion cycle It plays an important role and is one of the main participants in the material cycle and energy flow in the environment. It has the ability to enrich a variety of trace elements. The bacteria are rich in nutrients, high in protein content, and rich in various biologically active substances, and the active strains Contains certain disease-resistant and stress-resistant substances, and has strong adaptability, can tolerate high-concentration organic wastewater and strong decomposition and transfor...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12N1/20A23K1/16C12R1/01
CPCC12N1/20
Inventor 刘德海解复红权淑静王跃先殷圆圆韩磊郝益民马焕谢红霞吴华军杨东杰刘海军丁芳贾彬陈国参
Owner HENAN ACAD OF SCI INST OF BIOLOGY LIABILITY
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