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Decomposing inoculant for corn straw and preparation method of decomposing inoculant

A corn stalk and decomposing agent technology, applied in the field of microbial compound preparations, can solve problems such as affecting the number of microorganisms and increasing the content of fungal spores

Active Publication Date: 2018-03-16
SHANGHAI CHUANGBO ECOLOGICAL ENG
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0003] In patents such as CN 103031254 B, a high-efficiency straw decomposing agent and its preparation process, CN 103146391 B, a straw decomposing agent and its production process, and CN 103966150 B, a crop straw decomposing bacterial agent, its use method and effect, etc. Fungi such as Trichoderma, Aspergillus or Rhizopus are generally used as the main components of straw decomposing agents, which will greatly increase the content of fungal spores in the soil during use and affect the number of other types of microorganisms in farmland soil

Method used

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  • Decomposing inoculant for corn straw and preparation method of decomposing inoculant
  • Decomposing inoculant for corn straw and preparation method of decomposing inoculant
  • Decomposing inoculant for corn straw and preparation method of decomposing inoculant

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Embodiment 1

[0033] This embodiment is a preparation process of a preferred corn stalk decomposing agent.

[0034] Step 1: Cultivate Bacillus licheniformis, Bacillus megaterium, Streptomyces cellulosus and Rhodopseudomonas palustris;

[0035] (1) Transfer Bacillus licheniformis and Bacillus megaterium into nutrient broth medium for shaking culture respectively, the culture temperature is 37°C, the rotation number of the shaking culture is 200rpm, the culture time is 36h, and then put into Store in refrigerator at 2-4°C until use.

[0036] (2) Transfer Streptomyces cellulose into Gaoshi No. 1 liquid medium for shaking culture, the temperature of the culture is 28°C, the rotation speed of the shaking culture is 200rpm, the time of the culture is 66h, and then put into Store in refrigerator at 2-4°C until use.

[0037] (3) Transfer Rhodopseudomonas palustris into ATYP liquid medium for static culture at 30° C. for 72 hours, and then store in a 2-4° C. refrigerator for later use.

[0038] T...

Embodiment 2

[0042] This embodiment is another preferred preparation process of corn stalk decomposing agent.

[0043] Step 1: Cultivate Bacillus licheniformis, Bacillus megaterium, Streptomyces cellulosus and Rhodopseudomonas palustris;

[0044] (1) Transfer Bacillus licheniformis and Bacillus megaterium into nutrient broth medium for shaking culture respectively, the culture temperature is 30°C, the rotation number of the shaking culture is 220rpm, the culture time is 48h, and then put into Store in refrigerator at 2-4°C until use.

[0045] (2) Transfer Streptomyces cellulosus into Gaoshi No. 1 liquid medium for shaking culture, the temperature of the culture is 30°C, the rotation speed of the shaking culture is 180rpm, the time of the culture is 60h, and then put into Store in refrigerator at 2-4°C until use.

[0046] (3) Transfer Rhodopseudomonas palustris into ATYP liquid medium for static culture at 35° C. for 48 hours, and then store in a refrigerator at 2-4° C. for later use.

...

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Abstract

The invention provides a decomposing inoculant for corn straw and a preparation method of the decomposing inoculant. The decomposing inoculant has the advantages that the decomposing inoculant does not contain fungi and can effectively promote the decomposing of the corn straw without supplementing extra nitrogen sources; Bacillus licheniformis, Bacillus megaterium, Streptomyces cellulosae and Rhodopseudomonas palustris in the complex microbial inoculant has good decomposing ability to livestock and poultry manure and the corn straw, and the Bacillus and the Streptomyces can also generate metabolite inhibiting the fungi and can effectively inhibit pathogenic bacteria in soil and on the corn straw returning to the field so as to achieve a disease control effect.

Description

technical field [0001] The invention relates to a microbial compound preparation, in particular to a corn stalk decomposing agent and a preparation method thereof. Background technique [0002] In recent years, as governments at all levels have paid more and more attention to environmental protection, the proportion of corn stalks that have been incinerated and returned to the field has become higher and higher. However, due to the longer natural rotting time of corn stalks, it will affect the next crop. Therefore, after the straw is returned to the field, a straw decomposing agent should be used to effectively promote the maturity of the corn straw, so that the organic matter and nitrogen, phosphorus, potassium and other elements in the straw can become nutrients for crop growth. [0003] In patents such as CN 103031254 B, a high-efficiency straw decomposing agent and its preparation process, CN 103146391 B, a straw decomposing agent and its production process, and CN 10396...

Claims

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Application Information

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IPC IPC(8): C12N1/20C05F17/00C12R1/10C12R1/11C12R1/465C12R1/01
CPCC12N1/20C05F17/20Y02W30/40
Inventor 江旸马正驰
Owner SHANGHAI CHUANGBO ECOLOGICAL ENG
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