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A method for identifying American ginseng using a specific peptide group

A technology of meat ginseng and peptides, applied in the biological field, can solve problems such as lack of technical level and deceiving consumers

Active Publication Date: 2017-11-10
INSPECTION & QUARANTINE TECH CENT SHANDONG ENTRY EXIT INSPECTION & QUARANTINE BUREAU
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0004] Due to the lack of a particularly effective method of identifying sea cucumber species at present, the method of identifying sea cucumber species is only based on traditional appearance observation, lacking technical support, resulting in many unscrupulous merchants cheating consumers by shoddy goods and false ones. Therefore, it is necessary to An efficient and accurate method for identifying sea cucumbers

Method used

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  • A method for identifying American ginseng using a specific peptide group
  • A method for identifying American ginseng using a specific peptide group
  • A method for identifying American ginseng using a specific peptide group

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0094] Example 1, Sequence Source and Alignment Information of Specific Polypeptides

[0095] 1. The above polypeptides SEQ ID NO.1: EIAALAPPTMK; SEQ ID NO.2: LCYVFLDFEQEMATAASSSSLEK; SEQ ID NO.3: CDETLFQPSFIGMESAGIHETTYNSIMK all come from the protein actin isoform 2 [Holothria glaberrima], and its Accesion number on NCBI is gi|215882299 . After mass spectrometry analysis, the peptides SEQ ID NO.1: EIAALAPPTMK; SEQ ID NO.2: LCYVFLDFEQEMATAASSSSLEK; SEQ ID NO.3: CDETLFQPSFIGMESAGIHETTYNSIMK do exist in American ginseng with good peak shape and high intensity.

[0096] figure 1 It is the chromatogram of polypeptide EIAALAPPTMK in American ginseng, its m / z is 571.3.

[0097] figure 2 It is the chromatogram of polypeptide LCYVFLDFEQEMATAASSSSLEK in American ginseng, its m / z is 876.1.

[0098] image 3 is the chromatogram of polypeptide CDETLFQPSFIGMESAGIHETTYNSIMK in American ginseng, its m / z is 1069.5.

[0099] 2. The above polypeptide SEQ ID NO.4: ETYSIYIYK comes from the...

Embodiment 2

[0105] Example 2, Sequence Source and Alignment Information of Specific Polypeptides

[0106] 1. The above polypeptides SEQ ID NO.7: PYDDVLDPAYVISSR; SEQ ID NO.8: IMDDVLDPAYVISSR are all derived from the protein arginine kinase [Apostichopus japonicus], and its Accesion number on NCBI is gi|4586462. After mass spectrometry analysis, the peptides SEQ ID NO.7: PYDDVLDPAYVISSR; SEQ ID NO.8: IMDDVLDPAYVISSR do exist in American ginseng, and the peak shape is good and the intensity is high.

[0107] Figure 7 It is the chromatogram of polypeptide PYDDVLDPAYVISSR in American ginseng, its m / z is 855.4.

[0108] Figure 8 is the chromatogram of the polypeptide IMDDVLDPAYVISSR in American ginseng, its m / z is 565.3.

[0109] 2. The above polypeptide SEQ ID NO.9: DVITGEELFSDSYK; SEQ ID NO.10: LVDDLYWR comes from the protein translationally controlled tumor protein [Stichopus monotuberculatus], and its Accesion number on NCBI is gi|659902918. After mass spectrometry analysis, the pept...

Embodiment 3

[0116] Example 3, Sequence Source and Alignment Information of Specific Polypeptides

[0117] 1. The above polypeptides SEQ ID NO.13: DAGVIAGLQVLR; SEQ ID NO.14: SEIHELVLVGGSTR; SEQ ID NO.15: VEIESFFDGEDFSETLTR are all derived from heat shock protein 70 [Apostichopus japonicus], and its Accesion number on NCBI is gi|194465888. After mass spectrometry analysis, the peptides SEQ ID NO.113: DAGVIAGLQVLR; SEQ ID NO.14: SEIHELVLVGGSTR; SEQ ID NO.15: VEIESFFDGEDFSETLTR do exist in American ginseng with good peak shape and high intensity.

[0118] Figure 13 It is the chromatogram of polypeptide DAGVIAGLQVLR in American ginseng, its m / z is 606.4.

[0119] Figure 14 It is the chromatogram of the polypeptide SEIHELVLVGGSTR in American ginseng, and its m / z is 748.9.

[0120] Figure 15 is the chromatogram of the polypeptide VEIESFFDGEDFSETLTR in American ginseng, and its m / z is 1061.0.

[0121] 2. The above peptides SEQ ID NO.16: DDLVNNLGTIAK; SEQ ID NO.17: EEAYDYLEPDTIEQLVK; SEQ ...

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Abstract

The invention relates to a method for identifying isostichopus badionotus through specific peptide fragment sets and marker peptide. When one set of marker peptide is used independently or combined for use, the marker peptide can be used for identifying the isostichopus badionotus. The marker peptide is a low-homology polypeptide fragment of isostichopus badionotus protein, the low-homology polypeptide fragment means that the peptide fragment is particular to the isostichopus badionotus, and the length of the polypeptide fragment is 5-30 pieces of amino acid. Even though it is found that part of the protein is consistent with plant or bacteria and other species, when the isostichopus badionotus is compared with thelenota ananas, acaudina molpadioides, stichopus japonicas and isostichopus fuscus, the marker peptide is particular to the isostichopus badionotus.

Description

technical field [0001] The invention belongs to the field of biotechnology, and in particular relates to a method for identifying American ginseng (Isostichopus badionotus) by using a specific peptide group. Background technique [0002] Sea cucumbers are rich in acidic mucopolysaccharides, which have the effects of immunomodulators, anticoagulants, blood sugar control and blood fat reduction on the human body. Therefore, sea cucumber enjoys the reputation of "treasure house of nutrition", and modern people regard sea cucumber as "the first of the eight delicacies of seafood". There are many kinds of sea cucumbers in the market, and the prices of different sea cucumbers vary greatly due to their different nutritional value and taste. American meat ginseng is a kind of sea cucumber. It grows naturally in cold waters. Its growth period is long, generally 7-10 years, so its nutritional value is extremely high. In addition to being rich in protein and various trace elements pos...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): C07K14/435
CPCC07K7/06C07K7/08C07K14/43509C12N9/0008C12N9/1223C12Q1/48C12Y102/01009C12Y207/03003G01N30/02G01N30/06G01N30/14G01N30/72G01N33/6842G01N33/6848G01N2030/067G01N2030/146G01N2333/9123G01N2410/00G01N2560/00G01N2570/00
Inventor 张晓梅张鸿伟梁成珠鲍蕾王境堂
Owner INSPECTION & QUARANTINE TECH CENT SHANDONG ENTRY EXIT INSPECTION & QUARANTINE BUREAU
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