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Mechanical extraction method for chick embryo myoblasts

A technology of myoblasts and chicken embryos, which is applied in the field of bioengineering to achieve the effects of high survival rate, low price and cost saving

Inactive Publication Date: 2016-08-24
YANGZHOU XIANGLONG POULTRY DEV CO LTD +1
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

There are few domestic reports on the in vitro culture and biological characteristics of poultry myoblasts

Method used

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Examples

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Effect test

Embodiment

[0029] Take 6 well-developed 12-embryo chicken embryos, spray the eggshells with 75% alcohol, gently tap the air chamber with the blunt tip of the tweezers, and then use the curved tweezers to gently pinch the eggshell away from the air chamber , lift off the buffy membrane, take out the chicken embryo with small curved tweezers and put it into the ultra-clean bench (put it on the filter paper sterilized by ultraviolet light), wipe off the skin of the chicken abdomen with the blunt side of the curved tweezers, and expose the breast muscles on both sides Finally, take out the pectoralis major muscle with straight scissors and put it into a petri dish with D-Hank's added. Wash the cut pectoralis major muscle three times with D-Hank's, then suck D-Hank's off, press the muscle with one tweezer, and tear the muscle with the other curved tweezer to break it into a loose shape, and then use scissors Cut up the pectoralis major muscle, add 2-3ml cell culture medium to the chopped musc...

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PUM

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Abstract

The invention relates to a cell extraction method in the technical field of bioengineering, in particular to an extraction method for chick embryo myoblasts. The method includes the following steps that firstly, chick embryos are sterilized, and pectoralis major is taken and put into a D-Hank's culture dish; secondly, the pectoralis major is trimmed; thirdly, the pectoralis major is oscillated and suspended through a vortex oscillator; fourthly, filtration and centrifugation are carried out; fifthly, cell plate laying and differential attachment are carried out; sixthly, digestion is carried out to obtain the myoblasts; seventhly, counting and plate planting are carried out. By means of the extraction method for the chick embryo myoblasts, namely, the mechanical oscillation extraction method for the chick embryo myoblasts, it is guaranteed that the purity, number and vitality of the extracted myoblasts are higher than those of myoblasts extracted through other methods, and a foundation is laid for further development of poultry muscle development regulation and control mechanism experiments.

Description

technical field [0001] The invention relates to a cell extraction method in the technical field of bioengineering, in particular to a method for extracting chicken embryo myoblasts. Background technique [0002] Muscle tissue contains different cell subsets, including myoblasts (Vanden et al., 2004), muscle satellite cells (Mauro et al., 1961), pluripotent precursor cells (Jiang et al., 2002), Pluripotent stem cells (Caseet a1., 2005) and so on. Muscle-derived stem cells have been widely proven to have lateral differentiation potential and can differentiate into tissue cell types of the three germ layers, including (adipocytes, bone cells, chondrocytes, endothelial cells, glial cells, and blood cells) (Sun et al. , 2004; Hwang et a1., 2004; Farace et a1., 2004). Myoblasts are the main muscle stem cells, the main seed cells for muscle regeneration and repair, and originate from the stem cells of the embryonic mesoderm. Myoblasts include myoblasts in the embryo and satellit...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12N5/073C12N5/077
CPCC12N5/0603C12N5/0658C12N2509/00C12N2509/10
Inventor 赵振华李春苗黄华云黎寿丰王钱保梁忠刘一帆赵东伟卢建李新
Owner YANGZHOU XIANGLONG POULTRY DEV CO LTD
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