Running water type exposure device applied to small aquatic organisms

Abstract

The invention discloses a running water type exposure device applied to small aquatic organisms. The running water type exposure device comprises a tested substance dilute solution system, a peristaltic pump, a constant-temperature water bath device and an organism exposure system, wherein the organism exposure system comprises a plurality of treatment group exposure cans, a solvent control exposure can and a blank controlexposure can; a stirring device is arranged at the bottom of each exposure can; a support block is arranged on the inner wall of each exposure can; a support plate is arranged on each support block; a plurality of through holes are formed in each support plate; a sleeve ring is arranged at the edge of each through hole; the outer circular surface of each sleeve ring is sleeved with cylindrical tubes to form an experiment cavity; each experiment cavity is communicated with an outside test solution via a sieve net; a second through hole is also formed in the central position of each support plate; the organism exposure system is placed in the constant-temperature water bath device. Through the structure design, the running water type exposure device can carry out an exposure test on the easily-volatile, easily-hydrolytic, easily-photolytic, easily-biodegradable and insoluble substances; the stability and the accuracy of the exposure concentration are ensured; and the small aquatic organisms cannot flow out of the exposure device along with the water flow.

Description

technical field [0001] The invention relates to the field of environmental toxicology of aquatic organisms, in particular to a flowing water exposure device for small aquatic organisms. Background technique [0002] The toxicity data of aquatic organisms such as fish and daphnia are important ecotoxicity test data that must be provided in the registration and management of pesticides and other chemicals in various countries. Different aquatic organisms have different requirements for the applicable toxicity exposure equipment due to their different individual sizes. Taking Daphnia as an example, Daphnia magna is a representative species of aquatic Daphnia. It is selected as a model organism for aquatic toxicity due to its short life history, large reproduction, large body size, and sensitivity to changes in environmental conditions. Water body exposure is closest to the natural living environment of daphnia, which can be divided into static water exposure, semi-static water ...

AI Technical Summary

Problems solved by technology

The static water exposure type does not need to replenish the stock solution, and long-term exposure experiments cannot be carried out; the concentration of the semi-static water exposure type will fluctuate repeatedly within a certain period of time, which will affect the experimental results; only the flowing water type exposure can ensure the accuracy of the experimental results

[0003] At present, there are still some difficulties in the stability of the system and the maintenance of the constant concentration of the system during the test period for some flow-type equipment for small organisms in the laboratory, and the high-level container siphon and the direct overflow method of the simple exposure device are used in the test. Potential for small exposed organisms to flow out of the test system with the water flow

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