Fast propagating method for atractylis lancea
A kind of Cangzhu, rapid technology, applied in the field of rapid propagation of Atractylodes atractylodes, can solve the problems of inconspicuous effect and great problems in survival rate, and achieve the effect of enhancing survival rate, simple method and high survival rate
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Embodiment 1
[0024] A method for rapid propagation of Atractylodes atractylodis, comprising the following steps:
[0025] 1) Selection and processing of seeds: choose large and plump Atractylodes atractylodes seeds, disinfect with potassium permanganate with a mass concentration of 10%, soak in alcohol with a volume concentration of 75% for 30 minutes after 30 minutes, and use a mass concentration of 75% for 30 minutes. Soak in 3% ammonia water for 10 minutes, then rinse with sterile water;
[0026] 2) Seedling cultivation: Inoculate the Atractylodes atractylodes seeds treated in step 1) on the germination medium and cultivate them for 1 to 2 weeks to grow seedlings;
[0027] 3) Induction of clustered buds: cutting off the hypocotyls of the seedlings and inserting them into the differentiation medium for culturing to obtain clustered shoots, dividing the clustered shoots into individual plants and continuously culturing for 3 generations on the differentiation medium to obtain regenerated ...
Embodiment 2
[0039] A method for rapid propagation of Atractylodes atractylodis, comprising the following steps:
[0040] 1) Selection and processing of seeds: choose large and plump Atractylodes atractylodes seeds, disinfect with potassium permanganate with a mass concentration of 5%, soak in alcohol with a volume concentration of 75% for 20 minutes after 30 minutes, and use a mass concentration of Soak in 3% ammonia water for 5 minutes, then rinse with sterile water;
[0041] 2) Seedling cultivation: Inoculate the Atractylodes atractylodes seeds treated in step 1) on the germination medium and cultivate them for 1 to 2 weeks to grow seedlings;
[0042] 3) Induction of clustered buds: cutting off the hypocotyls of the seedlings and inserting them into the differentiation medium for culturing to obtain clustered shoots, dividing the clustered shoots into individual plants and continuously culturing for 3 generations on the differentiation medium to obtain regenerated plants;
[0043] 4) r...
Embodiment 3
[0054] A method for rapid propagation of Atractylodes atractylodis, comprising the following steps:
[0055] 1) Selection and processing of seeds: choose large and plump Atractylodes atractylodes seeds, disinfect with potassium permanganate with a mass concentration of 7%, soak in alcohol with a volume concentration of 75% for 25 minutes after 30 minutes, and use a mass concentration of 7% for 25 minutes. Soak in 3% ammonia water for 7 minutes, then rinse with sterile water;
[0056] 2) Seedling cultivation: Inoculate the Atractylodes atractylodes seeds treated in step 1) on the germination medium and cultivate them for 1 to 2 weeks to grow seedlings;
[0057] 3) Induction of clustered buds: cutting off the hypocotyls of the seedlings and inserting them into the differentiation medium for culturing to obtain clustered shoots, dividing the clustered shoots into individual plants and continuously culturing for 3 generations on the differentiation medium to obtain regenerated pla...
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