Fast propagating method for atractylis lancea

A kind of Cangzhu, rapid technology, applied in the field of rapid propagation of Atractylodes atractylodes, can solve the problems of inconspicuous effect and great problems in survival rate, and achieve the effect of enhancing survival rate, simple method and high survival rate

Active Publication Date: 2016-10-12
JIANGSU MAODISHANDAO CHINESE MEDICINAL HERBAL PLANTING
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

[0002] Atractylodes atractylodis is a commonly used traditional Chinese medicine. It has the functions of drying dampness and invigorating the spleen, expelling wind and cold, and improving eyesight. It is an endangered medicinal plant. According to the survey, most of the atractylodes currently on the market are Atractylodes atractylodes. Atractylodes atractylodes is rarely seen, so in order to solve the rapid reproduction of Atractylodes atractylodes, there are many breeding methods, but the effect is not obvious, and the survival rate is also a big problem

Method used

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Experimental program
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Effect test

Embodiment 1

[0024] A method for rapid propagation of Atractylodes atractylodis, comprising the following steps:

[0025] 1) Selection and processing of seeds: choose large and plump Atractylodes atractylodes seeds, disinfect with potassium permanganate with a mass concentration of 10%, soak in alcohol with a volume concentration of 75% for 30 minutes after 30 minutes, and use a mass concentration of 75% for 30 minutes. Soak in 3% ammonia water for 10 minutes, then rinse with sterile water;

[0026] 2) Seedling cultivation: Inoculate the Atractylodes atractylodes seeds treated in step 1) on the germination medium and cultivate them for 1 to 2 weeks to grow seedlings;

[0027] 3) Induction of clustered buds: cutting off the hypocotyls of the seedlings and inserting them into the differentiation medium for culturing to obtain clustered shoots, dividing the clustered shoots into individual plants and continuously culturing for 3 generations on the differentiation medium to obtain regenerated ...

Embodiment 2

[0039] A method for rapid propagation of Atractylodes atractylodis, comprising the following steps:

[0040] 1) Selection and processing of seeds: choose large and plump Atractylodes atractylodes seeds, disinfect with potassium permanganate with a mass concentration of 5%, soak in alcohol with a volume concentration of 75% for 20 minutes after 30 minutes, and use a mass concentration of Soak in 3% ammonia water for 5 minutes, then rinse with sterile water;

[0041] 2) Seedling cultivation: Inoculate the Atractylodes atractylodes seeds treated in step 1) on the germination medium and cultivate them for 1 to 2 weeks to grow seedlings;

[0042] 3) Induction of clustered buds: cutting off the hypocotyls of the seedlings and inserting them into the differentiation medium for culturing to obtain clustered shoots, dividing the clustered shoots into individual plants and continuously culturing for 3 generations on the differentiation medium to obtain regenerated plants;

[0043] 4) r...

Embodiment 3

[0054] A method for rapid propagation of Atractylodes atractylodis, comprising the following steps:

[0055] 1) Selection and processing of seeds: choose large and plump Atractylodes atractylodes seeds, disinfect with potassium permanganate with a mass concentration of 7%, soak in alcohol with a volume concentration of 75% for 25 minutes after 30 minutes, and use a mass concentration of 7% for 25 minutes. Soak in 3% ammonia water for 7 minutes, then rinse with sterile water;

[0056] 2) Seedling cultivation: Inoculate the Atractylodes atractylodes seeds treated in step 1) on the germination medium and cultivate them for 1 to 2 weeks to grow seedlings;

[0057] 3) Induction of clustered buds: cutting off the hypocotyls of the seedlings and inserting them into the differentiation medium for culturing to obtain clustered shoots, dividing the clustered shoots into individual plants and continuously culturing for 3 generations on the differentiation medium to obtain regenerated pla...

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Abstract

The invention discloses a fast propagating method for atractylis lancea. The method comprises the following steps of seed selection and treatment, wherein large and plump atractylis lancea seeds are selected and disinfected with potassium permanganate with the concentration of 5% to 10%, the seeds are soaked with 75% ethyl alcohol for 20-30 minutes 30 minutes later and soaked with 3% ammonium hydroxide for 5-10 minutes, and then the seeds are thoroughly washed with aquae sterilisata; seedling cultivation, wherein a sprouting medium is inoculated with the atractylis lancea seeds for culture for 1-2 weeks, and seedlings grow out; multiple shoot induction, wherein plumular axes of the seedlings are cut off, a differential medium is inoculated with the plumular axes for culture, multiple shoots are obtained, the multiple shoots are divided into single plants to be continuously cultured on the differential medium for 3 generations, and regenerated plants are obtained; root induction, wherein the regenerated plants are transplanted to a rooting medium for rooting induction, and plants with developed root systems are obtained; transplanting, wherein the plants with the developed root systems are transplanted into a test field for field management and grow under natural conditions. According to the method, explant is selected for propagation, and by means of the treatment method, the survival rate of the atractylis lancea seedlings is increased.

Description

technical field [0001] The invention belongs to the field of plant planting, and in particular relates to a rapid propagation method of atractylodes atractylodes. Background technique [0002] Atractylodes atractylodis is a commonly used traditional Chinese medicine. It has the functions of drying dampness and invigorating the spleen, expelling wind and cold, and improving eyesight. It is an endangered medicinal plant. According to the survey, most of the atractylodes currently on the market are Atractylodes atractylodes. Cangzhu is rarely seen, so in order to solve the rapid reproduction of Atractylodes atractylodes, there are many breeding methods, but the effect is not obvious, and the survival rate is also very problematic. Contents of the invention [0003] Purpose of the invention: The purpose of the invention is to provide a method for rapid propagation of Atractylodes atractylodes, which has high germination rate, short cultivation time, strong operability, high ap...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A01H4/00
CPCA01H4/00
Inventor 经守萄
Owner JIANGSU MAODISHANDAO CHINESE MEDICINAL HERBAL PLANTING
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