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Light sheet microscopic imaging conversion apparatus

A technology for microscopic imaging and conversion devices, which can be used in measuring devices, fluorescence/phosphorescence, and material analysis through optical means, and can solve the problems that an inverted microscope cannot be converted into a light-sheet imaging system, and achieve the effect of reducing costs

Active Publication Date: 2016-10-26
HUAZHONG UNIV OF SCI & TECH
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  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

[0004] Aiming at the above defects or improvement needs of the prior art, the present invention provides a light sheet microscopic imaging conversion device, the purpose of which is to make the relative movement between the miniaturized laser light sheet generation device and the sample in the vertical direction, so that the use Ordinary inverted fluorescence microscopes can collect high-resolution axial fluorescence images that can stack stereoscopic images, thereby solving the technical problem that existing inverted microscopes cannot be converted into light-sheet imaging systems

Method used

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Embodiment 1

[0031] The light sheet microscopic imaging conversion device provided by the invention, such as figure 1 As shown, the light sheet microscopic imaging conversion device provided by the present invention includes: a light sheet laser light source 1, a sample holder 2, and a mover 3, and the preferred solution also includes a two-dimensional mobile platform; the light sheet laser light source 1 , to generate a light sheet laser, which is irradiated horizontally on the sample 4 fixed on the sample holder 2; the mover 3 is connected with the sample holder 2, and is used to drive the sample holder 2 to move in the vertical direction , the two-dimensional moving platform is connected with a vertical motion for providing two-dimensional movement in a horizontal plane.

[0032] The light sheet laser light source 1 includes a light source 11 and a light sheet generation unit 12, the light source 11 is used to generate collimated laser light, and the light sheet generation unit 12 is us...

Embodiment 2

[0041] Heart development of transgenic fish embryos using FLK-GFP as observations. Flik fluorescent protein is expressed in endothelial cells and cardiomyocytes, so the fish's circulatory system, including blood vessels, and the heart emit green fluorescence under the action of excitation light. If observed with an ordinary wide-field illuminated inverted fluorescence microscope, due to extensive out-of-focus light irradiation, the tissues around the heart are excited to fluoresce, the entire inverted image has low contrast and serious light pollution, and the cross-sectional image from the heart is not obvious. Such as Figure 4 shown on the upper left side of the . After simply installing a light-sheet microscopic imaging conversion device, using the ultra-thin light-sheet illumination generated by the accessory can almost completely eliminate the pollution of out-of-focus light, and the obtained heart section image is clear and high-contrast, such as Figure 4 shown on th...

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Abstract

The invention discloses a light sheet microscopic imaging conversion apparatus. The light sheet microscopic imaging conversion apparatus comprises a light sheet laser light source, a sample clamping device and a motion device; the light sheet laser light source generates light sheet laser which is horizontally irradiated onto a sample which is fixedly arranged on the sample clamping device; and the motion device is connected with the sample clamping device and used for driving the sample clamping device to move in a direction forming an angle less than 15 degrees with the vertical direction. The light sheet microscopic imaging conversion apparatus is a small-sized conversion apparatus with low cost; the light sheet microscopic imaging conversion apparatus is installed on an ordinary inverted fluorescence microscope, so that the light sheet microscopic imaging conversion apparatus has a light sheet microscopic imaging capacity; and a great breakthrough on the three-dimensional imaging can be realized by virtue of small modification, so that the three-dimensional imaging cost of a biological sample can be greatly reduced.

Description

technical field [0001] The invention belongs to the field of microscopic imaging, and more specifically relates to a light sheet microscopic imaging conversion device. Background technique [0002] Light sheet microscopy is a new type of microscopic imaging technology invented in this century. Compared with laser confocal scanning and ordinary wide-field fluorescence microscopy, it has many advantages such as low phototoxicity, high axial resolution, and large imaging dynamic range. Scope 3D viewing. Light sheet microscopy is different from traditional fluorescence microscopy, it uses an additional illumination path to generate an ultra-thin laser light sheet to illuminate the sample, and only the extremely thin plane illuminated by the light sheet in the sample will be excited to produce fluorescence, and then collect a series of fluorescence images with high axial resolution by setting the microscopic imaging optical path in a direction orthogonal to the light sheet illu...

Claims

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Application Information

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IPC IPC(8): G01N21/01G01N21/64
CPCG01N21/01G01N21/6458G01N2201/103
Inventor 费鹏刘胜江浩聂俊关泽一
Owner HUAZHONG UNIV OF SCI & TECH
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