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Method for measuring ratio of nicotine carbon, hydrogen and nitrogen stable isotopes in tobacco

A technology of isotope ratio and determination method, which is applied in the determination of hydrogen and nitrogen stable isotope ratio, and the field of nicotine carbon in tobacco, can solve the problems of complex nicotine extraction and purification process, etc., and achieves simple operation, high efficiency and good repeatability. Effect

Inactive Publication Date: 2016-10-26
CHINA NAT TOBACCO QUALITY SUPERVISION & TEST CENT
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  • Abstract
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Problems solved by technology

Jamin et al. used stable isotope technology to successfully partition tobacco leaves from South America, Europe, South Asia and Africa ( Plant, Cell Environ. 1997, 20(5) : 589-599.), however, this method requires complex nicotine extraction and purification processes, and only the isotope ratio determination of nicotine carbon and nitrogen in tobacco

Method used

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  • Method for measuring ratio of nicotine carbon, hydrogen and nitrogen stable isotopes in tobacco
  • Method for measuring ratio of nicotine carbon, hydrogen and nitrogen stable isotopes in tobacco
  • Method for measuring ratio of nicotine carbon, hydrogen and nitrogen stable isotopes in tobacco

Examples

Experimental program
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Effect test

Embodiment 1

[0029] 1. Instruments and reagents

[0030] Isotope mass spectrometer (AS1310 autosampler, Trace GC Ultra gas chromatograph, GC IsoLink combustion and thermal conversion device (combustion device is used to determine carbon and nitrogen in nicotine, thermal conversion device is used to determine hydrogen in nicotine) , Delta VAdvantage stable isotope ratio mass spectrometer. Thermo Fisher, USA); AE163 electronic balance (sensitivity: 0.0001g, Mettler, Switzerland); high-purity helium (99.999%, carrier gas); nicotine reference material comes from Indiana, USA University Isotope Laboratory, #2 (δ 13 C =7.72±0.02‰, δ 15 N=-5.94±0.15‰), #3 (δ 13 C=-30.05±0.02‰, δ 15 N=-33.62±0.18‰), #4 (δ 13 C=-2.06±0.02‰, δ 15 N=-15.49±0.13‰), #5 (δ 13 C=-29.63±0.01‰, δ 15 N=--6.03±0.04‰, δD=-161.30±1.7‰); n-hexane (chromatographically pure, TEDIA, USA); sodium hydroxide (analytical pure, Sinopharm); ultrapure water (Milli-Q Integral ultrapure Water system system), Y-8 speed control oscil...

Embodiment 2

[0048] According to the assay method described in Example 1, select a kind of tobacco sample B, record the δ of wherein nicotine 13 C, δD and δ 15 N is -31.17, -211.44 and -6.83, respectively.

Embodiment 3

[0050] According to the assay method described in Example 1, a kind of tobacco sample C is selected, and the δ of nicotine is recorded therein. 13 C, δD and δ 15 N were -32.97, -229.05 and -5.51, respectively.

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PUM

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Abstract

A method for measuring ratio of nicotine carbon, hydrogen and nitrogen stable isotopes in tobacco is characterized in that extracting nicotine from tobacco by using an n-hexane and sodium hydroxide solution system, performing liquid sample injection, separating the nicotine from other impurities in extract by using a gas chromatograph fitted with a capillary chromatographic column, analyzing carbon stable isotope ratio Delta 13C, hydrogen stable isotope ratio (Delta D) and nitrogen stable isotope ratio Delta 15N in the nicotine by using a gas chromatography and isotope ratio mass spectrograph (GC-IRMS), and calibrating and calculating the values Delta 12C, Delta D and Delta 15N of the nicotine in tobacco according to measured values Delta 12C, Delta D and Delta 15N in nicotine isotope standard matters. By using GC-IRMS technology, nicotine carbon, hydrogen and nitrogen stable isotope ratio in tobacco can be measured quickly and accurately, and a technique is provided for tracing tobacco products.

Description

technical field [0001] The invention belongs to the technical field of stable isotope analysis, in particular to a method for measuring the ratios of nicotine carbon, hydrogen and nitrogen stable isotopes in tobacco. Background technique [0002] Tobacco leaves are the basis of cigarette production in my country, and the intrinsic quality of tobacco leaves directly affects the quality of cigarette products. Affected by the environment of the producing area, such as longitude and latitude, altitude, temperature and humidity, precipitation, soil type and other factors, the quality of tobacco leaves in different planting areas varies greatly, and the style characteristics are very different. Making full use of the flavor characteristics of tobacco leaves from various regions in the formula of cigarette leaf groups and highlighting the style characteristics and flavor quality of cigarette brands is of great significance for making cigarette products bigger, more specialized and ...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): G01N30/02
CPCG01N30/02G01N2030/025
Inventor 韩书磊陈欢马潇付亚宁刘彤侯宏卫胡清源
Owner CHINA NAT TOBACCO QUALITY SUPERVISION & TEST CENT
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