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Kit for determining haptoglobin and preparation method thereof

A technology of binding globin and kit, applied in the fields of medicine and biochemistry, can solve the problems of low measurement accuracy and complicated operation, and achieve the effects of improving sensitivity, facilitating dispersion and stability

Inactive Publication Date: 2016-10-26
ANHUI IPROCOM BIOTECH CO LTD
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0007] The technical problem to be solved by the present invention is to provide a kit for measuring haptoglobin and its preparation method in order to overcome the defects of complicated operation and low measurement accuracy in the prior art

Method used

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  • Kit for determining haptoglobin and preparation method thereof

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0072] The kit of the present invention includes reagent R1 and reagent R2 two-liquid components independent of each other, wherein

[0073] Reagent R1:

[0074] Tris buffer 100 mmol / L

[0075] Polyethylene glycol-8000 30 g / L

[0076] Tween-80 15ml / L

[0077] Sodium chloride 150 mmol / L

[0078] Sodium azide 0.4 g / L

[0079] The solvent is purified water

[0080] Reagent R2:

[0081] Tris buffer 100 mmol / L

[0082] Glycerin 20ml / L

[0083] Tween-80 14ml / L

[0084] Sodium chloride 200 mmol / L

[0085] Sodium azide 0.4 g / L

[0086] Anti-human haptoglobin antibody 10.0 ml / L

[0087] Its solvent is purified water.

Embodiment 2

[0089] The kit of the present invention includes reagent R1 and reagent R2 two-liquid components independent of each other, wherein

[0090] Reagent R1:

[0091] Tris buffer 185 mmol / L

[0092] Polyethylene glycol-2000 45 g / L

[0093] Polyoxyethylene phenyl ether 10.0ml / L

[0094] Potassium chloride 300 mmol / L

[0095] Sodium Benzoate 0.1g / L

[0096] The solvent is purified water

[0097] Reagent R2:

[0098] Tris buffer 185 mmol / L

[0099] Gum Arabic 10.0 ml / L

[0100] Polyoxyethylene phenyl ether 10.0ml / L

[0101] Potassium chloride 250 mmol / L

[0102] Sodium Benzoate 0.1 g / L

[0103] Anti-human haptoglobin antibody 18.0 ml / L

[0104] Its solvent is purified water.

Embodiment 3

[0106] Kit preparation and method of use

[0107] 1. Prepare the reagent according to the content of the following components:

[0108] Reagent R1:

[0109] Tris buffer 100 mmol / L

[0110] Polyethylene glycol-8000 30 g / L

[0111] Tween-80 15ml / L

[0112] Sodium chloride 150 mmol / L

[0113] Sodium azide 0.4 g / L

[0114] The solvent is purified water

[0115] Reagent R2:

[0116] Tris buffer 100 mmol / L

[0117] Glycerin 20ml / L

[0118] Tween-80 14ml / L

[0119] Sodium chloride 200 mmol / L

[0120] Sodium azide 0.4 g / L

[0121] Anti-human haptoglobin antibody 10.0 ml / L

[0122] The solvent is purified water;

[0123] 2. Parameter setting of automatic biochemical analyzer

[0124] (a) Detection temperature: 37°C;

[0125] (b) Detection wavelength: main wavelength 340nm, secondary wavelength 700nm;

[0126] (c) Reaction time: 10 minutes, among them, the incubation time is 5 minutes, measure and read the absorbance A1 immediately after adding the reagent R2, read the a...

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Abstract

The invention discloses a kit for determining haptoglobin and a preparation method thereof. The kit comprises dual-liquid components of a reagent R1 and a reagent R2 which are independent from each other. The reagent R1 is prepared from a buffer solution, an accelerator, a surface active agent, inorganic salt ions and a corrosion remover, and a solvent of the reagent R1 is purified water; the reagent R2 is prepared from a buffer solution, a suspending agent, a surface active agent, inorganic salt ions, a corrosion remover and haptoglobin antibodies, and a solvent of the reagent R2 is purified water. The preparation method comprises the steps that the reagents are prepared according to the component content; a to-be-tested sample is mixed with the reagent R1 and the reagent R2 to be subjected to a sufficient reaction; a fully automatic biochemical analyzer is used for determining an absorptivity difference obtained after the reaction; according to an absorptivity change value, the concentration of haptoglobin in the sample is worked out. The kit has the advantages of being convenient to operate, high in accuracy and the like.

Description

technical field [0001] The invention relates to the technical fields of medicine and biochemistry, in particular to a kit for measuring haptoglobin and a preparation method thereof. Background technique [0002] Haptoglobin, also known as haptoglobin, is an acidic glycoprotein with a molecular weight of 85,000. It is widely present in serum and other body fluids of humans and various mammals. In CAM electrophoresis and agarose gel electrophoresis, binding beads The protein is located in the α2 zone, and there are two pairs of peptide chains (α chain and β chain) in the molecule to form a tetramer of α2β2. Haptoglobin is mainly synthesized in the liver, and its degradation is also in the liver, with a half-life of about 3.5 to 4 days. [0003] The main function of haptoglobin is to combine with free hemoglobin to form a stable complex, which is then disposed of by the monocyte-macrophage system. Under normal circumstances, human red blood cells can remain in circulation desp...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): G01N33/68
CPCG01N33/68G01N2333/47
Inventor 蔡晓辉庄庆华吴铮徐运
Owner ANHUI IPROCOM BIOTECH CO LTD
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