Cycloastragenol extract and preparation method and application thereof

A technology of cycloastragenol and extract, which is applied in the field of medicine, can solve the problems of very few studies on cycloastragenol extract, and achieve the effects of slowing down aging damage, improving cell vitality, and anti-aging activity

Inactive Publication Date: 2016-11-09
ZHEJIANG UNIV OF TECH
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0005] So far, most of the work has focused on how to obtain high-purity CAG and what biological activity CAG single products have, and they are all based on astragaloside IV monomers, and there are very few related studies on cycloastragenol extracts.

Method used

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  • Cycloastragenol extract and preparation method and application thereof
  • Cycloastragenol extract and preparation method and application thereof
  • Cycloastragenol extract and preparation method and application thereof

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Experimental program
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Effect test

Embodiment 1

[0033] Embodiment 1 The preparation method of the astragaloside IV crude extract of the present invention:

[0034]The Radix Astragali used complies with the relevant provisions of the 2010 edition of the Chinese Pharmacopoeia. Take 30 g of Astragalus membranaceus coarse powder, add 10 times of 75% ethanol with pH of 13 and soak overnight, use the same solution to reflux extract twice, each time for 2 hours, filter, combine the filtrates, evaporate the solvent under reduced pressure, and then freeze-dry to obtain astragaloside IV The crude extract was 0.8084g, and the content of astragaloside IV was determined to be 0.7399% by HPLC detection.

Embodiment 2

[0035] Embodiment 2 The preparation method of astragaloside IV crude extract of the present invention:

[0036] The Radix Astragali used complies with the relevant provisions of the 2010 edition of the Chinese Pharmacopoeia. Take 30 g of Astragalus membranaceus coarse powder, add 10 times of 75% ethanol with pH of 12 and soak overnight, use the same solution to reflux extract twice, each time for 2 hours, filter, combine the filtrates, evaporate the solvent under reduced pressure and then freeze-dry to obtain astragaloside IV The crude extract was 0.8080g, and the content of astragaloside IV was determined to be 0.7381% by HPLC detection.

Embodiment 3

[0037] Embodiment 3 The preparation method of the astragaloside IV crude extract of the present invention:

[0038] The Radix Astragali used complies with the relevant provisions of the 2010 edition of the Chinese Pharmacopoeia. Take 30 g of Astragalus membranaceus coarse powder, add 10 times of 75% ethanol with pH 11 and soak overnight, use the same solution to reflux extract twice, each time for 2 hours, filter, combine the filtrates, evaporate the solvent under reduced pressure and then freeze-dry to obtain astragaloside IV The crude extract was 0.9088g, and the content of astragaloside IV was determined to be 0.6596% by HPLC detection.

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Abstract

The invention provides a cycloastragenol extract. A preparation method of the cycloastragenol extract comprises the steps that coarse astragalus membranaceus powder is extracted by using an alkaline alcohol water solution with a pH value of 11-13 to obtain a coarse astragaloside extract; the obtained coarse astragaloside extract is enriched and purified through macroporous resin to obtain an astragaloside extract; the obtained astragaloside extract is subjected to Smith degradation to obtain the cycloastragenol extract. For cycloastragenol extract preparation, an optimum extraction method is obtained through multiple times of tests conducted on the pH value, sodium periodate dosage and methanol concentration in the extraction conditions. The method is good in reproducibility, simple and easy to operate. The prepared cycloastragenol extract can obviously retard senility hurt of H2O2 to PC12 cells, and improve cell vitality, and the cycloastragenol extract obtained by adopting the preparation method has certain anti-aging activity and can be applied to preparation of anti-aging medicines and health-care products.

Description

(1) Technical field [0001] The invention relates to a cycloastragenol extract, a preparation method and application of the extract, and belongs to the field of medicine. (2) Background technology [0002] At present, the aging population of countries around the world is accelerating. According to statistics, in 2012, the elderly population aged 65 and above in my country accounted for 9.4% of the total population. Double the number of elderly people in 2012. In such a severe situation, human beings are facing enormous challenges. Aging and anti-aging have become a hot topic in medical discussion today. With the improvement of people's living standards and the continuous strengthening of the concept of returning to green life, how to effectively prevent aging and improve the quality of life has become an urgent problem that people need to solve. Therefore, people's demand for anti-aging related green health care products and plant extracts continues to increase. [0003] In...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C07J53/00A23L33/105A61P39/00
CPCC07J53/004
Inventor 张慧章诗迪颜继忠吕华伟沈芒芒
Owner ZHEJIANG UNIV OF TECH
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