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Bacillus amyloliquefaciens DGY 18 for degrading nicotine and application method thereof

A technology of amylolytic spores and application methods, applied in the direction of microorganism-based methods, applications, botany equipment and methods, etc., can solve the problems of high economic cost, time-consuming and labor-intensive, and poor effect of reducing nicotine, so as to reduce the content, The effect with better effect and good application prospect

Active Publication Date: 2016-11-16
CHINA TOBACCO HUNAN INDAL CORP
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

The first two methods are not only time-consuming and labor-intensive, but also have high economic costs, and the effect of reducing nicotine is not good. However, the use of microorganisms to reduce the nicotine content of cigarettes, improve the quality of tobacco leaves, and improve resource utilization are of great significance.

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0039] Cut tobacco extract formula: Weigh 10g cut tobacco powder, 13.3g K 2 HPO4·3H 2 O, 4g KH 2 PO 4 ·3H 2 O, 0.2gMgSO 4 ·7H 2 O, 1g maltose, 1g peptone, measure 0.5mL trace element solution (trace element solution is MnSO4 with 0.1mol / L hydrochloric acid 4 ·7H 2 O 0.4g, CaCl 2 2H 2 O 0.2g, FeSO 4 ·7H 2 O 0.2g was dissolved and the volume was adjusted to 100mL. ) into 500mL of distilled water, dilute to 1000mL with distilled water, subpackage, and sterilize.

[0040] Preparation of Bacillus amyloliquefaciens DGY18 bacterial suspension used for inoculation of cut tobacco extract: the pure strain was inserted into 5mL liquid nicotine medium, cultured on a shaker at 30°C and 200r / min for 2 days, and then the culture solution was 10000r / min Centrifugal 10min, collect thalline, with 0.9% physiological saline, the thalline after centrifugation is formulated into bacterial suspension (thalline concentration is 10 7 -10 8 CFU / mL).

[0041] Nicotine medium formula: K 2...

Embodiment 2

[0044] Preparation of Bacillus amyloliquefaciens DGY18 bacterial suspension applied to spraying on the leaves of tobacco plants: the pure strains were inserted into 100mL liquid nutrient medium at 30°C, shaken at 120r / min and cultured overnight, 5%-10% by volume The culture solution was added to 2.5 L of liquid nutrient medium and cultivated for 48 hours. Liquid nutrient-based formula: beef extract 5g, peptone 10g, sodium chloride 5g, water 1000mL, adjust the pH to 7.0-7.2. When the tobacco grows and enters the mature stage, the 6 tobacco leaves on the upper part of each tobacco plant are sprayed, and each tobacco plant is sprayed with 25mL bacterial liquid, and treated for 20 days (treatment once every 2 days, that is, the 1st, 4th, 7th, 10th, 13th, 1st, 4th, 7th, 10th, 13th) The 16th and 19th days were treated once respectively, and the treatment was carried out in the evening of the same day), and the tobacco leaves of each treated tobacco plant were sampled once respective...

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PUM

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Abstract

The invention discloses bacillus amyloliquefaciens DGY 18 for degrading nicotine and application method thereof. The conservation series number is CGMCC NO.11900. The bacillus amyloliquefaciens DGY 18 acts on a healthy tobacco plant, and within 20 days of a test, the degradation rate of nicotine in tobacco leaves on the upper portion is 30-40%. Bacillus amyloliquefaciens DGY 18 is used for degrading nicotine, which saves time and labor and has obvious economic benefits; the content of nicotine in the tobacco leaves on the upper portion can be controlled more effectively, a guarantee of the tobacco leaf raw material higher in usability is provided for development of low-tar cigarette products, usability of the tobacco leaves is improved, and therefore bacillus amyloliquefaciens DGY 18 has wide application prospects in tobacco processing and environment protection.

Description

technical field [0001] The invention relates to a Bacillus amyloliquefaciens DGY18 (Bacillus amyloliquefaciens DGY18) for degrading nicotine and a method for using the bacterium to degrade nicotine in shredded tobacco extract and upper tobacco leaves of tobacco plants, belonging to the technical field of microbial application. Background technique [0002] Nicotine, also known as nicotine, is the main alkaloid present in tobacco, accounting for more than 95% of the alkaloid content, and is also one of the main harmful components in cigarettes and tobacco leaves. The current tobacco production requires that the nicotine content in the tobacco leaves be moderate, but due to the application of a large amount of chemical fertilizers in the tobacco planting process in my country, the nicotine content in the upper tobacco leaves is too high, and the nicotine content in some upper tobacco leaves reaches more than 5%; Caused this part tobacco leaf to lose available value. It is esti...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12N1/20A24B15/20A01N63/00A01P21/00C12R1/07
CPCA01N63/00A24B15/20C12N1/205C12R2001/07
Inventor 易建华王修忠蒲文宣彭宇孙在军汪耀富张惠琳周曙光王东
Owner CHINA TOBACCO HUNAN INDAL CORP
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