Primary culture bud induction method of cork oak

Abstract

The invention discloses a primary culture bud induction method of a cork oak. The primary culture bud induction method is characterized in that a coppice shoot at the root of the cork oak is adopted as an explant, after asepsis of the explant, the explant is inoculated on a high-quality primary culture bud induction culture medium and is placed under the conditions of appropriate temperature, humidity and illumination intensity for cultivation so as to induce the germination of buds. The method overcomes the problems such as explant browning and vitrification in a bud induction process, a bud induction rate, a germination index and germination growth conditions are improved, and a great number of high-quality buds are obtained, so that the requirement of enrichment culture is met, and the establishment of a cork oak tissue culture intermediate propagation system is promoted.

Description

technical field

[0001] The invention relates to the asexual propagation technique of the cork oak, in particular to a bud induction method for the primary culture of the cork oak. Background technique

[0002] cork oak ( Quercus variabilis Bl ) Also known as Cork Quercus, Quercus vulgaris, and Quercus vulgaris, it is a tree of the genus Quercus in the family Fagaceae, with a broad oval crown, many trunks, taupe, deep longitudinal fissures, and a thick cork layer. The branchlets are light brown, the male inflorescences are born in the lower part of the current year's branches, and the female flowers are solitary or twin in the axils of the current year's branches. The flowering period is May; the fruit matures in September-October of the following year. It is an important timber tree species in China. Cork oak likes light and is often born on sunny slopes in mountains, but it is better for young trees to have side shade. Strong adaptability to climate and soil. It can wi...

Examples

Embodiment 1

[0021] Semi-lignified shoots germinated from the base of Quercus variabilis with a length of 6-7 cm and a diameter of 0.2-0.3 cm were selected as explants. Cut off the needles and leaves of Quercus cork, put them in a triangular flask, add detergent solution with a volume concentration of 1%, seal the bottle mouth with gauze, and wash it on a shaker with a rotation speed of 200 r / min for 10 min. Rinse under running water for 2 h, then wash twice with sterile water, transfer the explants to an ultra-clean workbench and sterilize them with 0.1% mercuric chloride solution for 10 min; then rinse with sterile water for 8~ After 10 times, the stems cut to 3.5 cm long were inoculated in the bud induction medium, and 2 stems were inoculated in each bottle.

[0022] The raw material content of the bud induction medium is: 1 / 3 improved WPM medium + 6-BA 1.0 mg / L + vitamin C 10 mg / L + glucose 5.0 g / L + sucrose 20.0 g / L. Wherein the improved WPM medium composition and volume to weight ra...

Embodiment 2

[0025] Semi-lignified shoots germinated from the base of Quercus variabilis with a length of 7-8 cm and a diameter of 0.2-0.3 cm were selected as explants. Cut off the needles and leaves of the cork oak, put them in a triangular flask, add a 2% volume concentration of detergent solution, seal the bottle mouth with gauze, and wash it for 10 minutes on a shaker with a rotation speed of 200 r / min. Rinse under running water for 1.5 h, then wash twice with sterile water, transfer the explants to an ultra-clean workbench and sterilize them with 0.1% mercury chloride solution for 10 min; then rinse with sterile water for 8~ After 10 times, the stems cut to 3.5 cm long were inoculated in the bud induction medium, and 2 stems were inoculated in each bottle.

[0026] The raw material content of the bud induction medium is: 1 / 3 improved WPM medium + 6-BA 1.5 mg / L + vitamin C 10 mg / L + glucose 5.0 g / L + sucrose 20.0 g / L. Wherein the improved WPM medium composition and volume to weight ra...

Embodiment 3

[0029] The semi-lignified shoots germinated from the base of Quercus variabilis with a length of 7-8 cm and a diameter of 0.3-0.4 cm were selected as explants. Cut off the needles and leaves of the cork oak, put them in a triangular flask, add a 4% volume concentration of detergent solution, seal the bottle mouth with gauze, and wash it for 15 minutes on a shaker with a rotation speed of 200 r / min. Rinse under running water for 1 h, then wash twice with sterile water, transfer the explants to an ultra-clean workbench and sterilize them with 0.1% mercuric chloride solution for 10 min; then rinse with sterile water for 8~ After 10 times, the stem segments cut to a length of 4.0 cm were inoculated in the bud induction medium, and 2 stem segments were inoculated in each bottle.

[0030] The raw material content of the bud induction medium is: 1 / 2 improved WPM medium + 6-BA 1.5 mg / L + vitamin C 10 mg / L + glucose 5.0 g / L + sucrose 20.0 g / L. Wherein the improved WPM medium compositi...

technical field

[0001] The invention relates to the asexual propagation technique of the cork oak, in particular to a bud induction method for the primary culture of the cork oak. Background technique

[0002] cork oak ( Quercus variabilis Bl ) Also known as Cork Quercus, Quercus vulgaris, and Quercus vulgaris, it is a tree of the genus Quercus in the family Fagaceae, with a broad oval crown, many trunks, taupe, deep longitudinal fissures, and a thick cork layer. The branchlets are light brown, the male inflorescences are born in the lower part of the current year's branches, and the female flowers are solitary or twin in the axils of the current year's branches. The flowering period is May; the fruit matures in September-October of the following year. It is an important timber tree species in China. Cork oak likes light and is often born on sunny slopes in mountains, but it is better for young trees to have side shade. Strong adaptability to climate and soil. It can wi...

Examples

Embodiment 1

[0021] Semi-lignified shoots germinated from the base of Quercus variabilis with a length of 6-7 cm and a diameter of 0.2-0.3 cm were selected as explants. Cut off the needles and leaves of Quercus cork, put them in a triangular flask, add detergent solution with a volume concentration of 1%, seal the bottle mouth with gauze, and wash it on a shaker with a rotation speed of 200 r / min for 10 min. Rinse under running water for 2 h, then wash twice with sterile water, transfer the explants to an ultra-clean workbench and sterilize them with 0.1% mercuric chloride solution for 10 min; then rinse with sterile water for 8~ After 10 times, the stems cut to 3.5 cm long were inoculated in the bud induction medium, and 2 stems were inoculated in each bottle.

[0022] The raw material content of the bud induction medium is: 1 / 3 improved WPM medium + 6-BA 1.0 mg / L + vitamin C 10 mg / L + glucose 5.0 g / L + sucrose 20.0 g / L. Wherein the improved WPM medium composition and volume to weight ra...

Embodiment 2

[0025] Semi-lignified shoots germinated from the base of Quercus variabilis with a length of 7-8 cm and a diameter of 0.2-0.3 cm were selected as explants. Cut off the needles and leaves of the cork oak, put them in a triangular flask, add a 2% volume concentration of detergent solution, seal the bottle mouth with gauze, and wash it for 10 minutes on a shaker with a rotation speed of 200 r / min. Rinse under running water for 1.5 h, then wash twice with sterile water, transfer the explants to an ultra-clean workbench and sterilize them with 0.1% mercury chloride solution for 10 min; then rinse with sterile water for 8~ After 10 times, the stems cut to 3.5 cm long were inoculated in the bud induction medium, and 2 stems were inoculated in each bottle.

[0026] The raw material content of the bud induction medium is: 1 / 3 improved WPM medium + 6-BA 1.5 mg / L + vitamin C 10 mg / L + glucose 5.0 g / L + sucrose 20.0 g / L. Wherein the improved WPM medium composition and volume to weight ra...

Embodiment 3

[0029] The semi-lignified shoots germinated from the base of Quercus variabilis with a length of 7-8 cm and a diameter of 0.3-0.4 cm were selected as explants. Cut off the needles and leaves of the cork oak, put them in a triangular flask, add a 4% volume concentration of detergent solution, seal the bottle mouth with gauze, and wash it for 15 minutes on a shaker with a rotation speed of 200 r / min. Rinse under running water for 1 h, then wash twice with sterile water, transfer the explants to an ultra-clean workbench and sterilize them with 0.1% mercuric chloride solution for 10 min; then rinse with sterile water for 8~ After 10 times, the stem segments cut to a length of 4.0 cm were inoculated in the bud induction medium, and 2 stem segments were inoculated in each bottle.

[0030] The raw material content of the bud induction medium is: 1 / 2 improved WPM medium + 6-BA 1.5 mg / L + vitamin C 10 mg / L + glucose 5.0 g / L + sucrose 20.0 g / L. Wherein the improved WPM medium compositi...