Method for improving purity of hybrid rice seeds through cytoplasm-controlled leaf color mutant

Abstract

The invention discloses a method for improving the purity of hybrid rice seeds through a cytoplasm-controlled leaf color mutant. The method comprises the steps that the cytoplasm-controlled leaf color mutant obtained through ethylmethane sulfonate (EMS) mutagenesis is taken as a female parent to cross with a three-line maintainer line, later generations continuously back-cross with the three-line maintainer line, and a serial cytoplasm-controlled leaf color marker maintainer line is obtained; the serial cytoplasm-controlled leaf color marker maintainer line crosses and back-crosses a sterile line, and a corresponding matched sterile line is bred; the cytoplasm-controlled leaf color marker maintainer line and the corresponding sterile line are subjected to large-field propagation, and the maintainer lines in the sterile line group are removed according to a leaf color marker; the propagated sterile systems and a restoring line are subjected to large-field seed production. According to the method, the sterile lines and the maintainer lines are easily separated by the cytoplasm gene-controlled leaf color mutant, the maintainer lines mixed in the sterile lines can be removed from the source of sterile line propagation, and then the purity of the hybrid rice seeds is effectively improved.

Description

technical field [0001] The invention belongs to the technical field of crop breeding, in particular to a method for improving the purity of hybrid rice seeds by using cytoplasm to control leaf color mutants. Background technique [0002] Leaf color mutants are a common trait mutation in higher plants. Leaf color mutation is a genetic phenomenon, which is related to abnormalities in the shape, structure, composition and physiological state of chloroplasts. Most studies have shown that leaf color mutations are caused by recessive nuclear gene mutations, and very few are the result of cytoplasmic gene mutations. Leaf color mutation is an easily identifiable trait mutation. As a marker trait combined with the hybrid rice CMS, the CMS self-bred seeds can be removed according to the difference in rice leaf color, ensuring that the hybrid F 1 purity. There have been many reports in recent years on the research on rice leaf color markers. Ren Guangjun et al. first transferred th...

AI Technical Summary

Problems solved by technology

It is known that the purity of the propagated sterile line is not high because it contains maintainer seeds, and the leaf color markers controlled by nuclear genes cannot distinguish between the two

Images