A kind of method utilizing ems mutagenesis to create watermelon mutant

Abstract

The invention belongs to the technical field of watermelon breeding, and in particular relates to a method for using EMS mutagenesis to create watermelon mutants. The method includes collecting fully open male flower anthers, fully stirring the anthers with EMS-paraffin oil solution to obtain EMS-treated pollen, dipping the pollen to pollinate the open female flowers on the same day, and spraying evenly the female flowers pollinated by EMS pollen with an appropriate concentration of gualin Ovary, harvesting and planting M0 generation seeds, and obtaining watermelon EMS mutant individuals with rich variation in M1 generation. Various mutants such as male sterility, dwarfing, tetraploid, and leaf deformity can be obtained through the present invention, which effectively solves the problems of narrow genetic background, less genetic variation, lack of excellent germplasm, etc. in watermelon functional gene research and variety breeding. The low efficiency of germplasm creation and other technical bottlenecks can be used to construct a watermelon mutant library, which is convenient for functional gene research, can greatly promote the mining and application of excellent watermelon genes, and provide material support for the breeding of excellent new varieties.

Description

A method for creating watermelon mutants by EMS mutagenesis technical field The present invention relates to watermelon breeding technical field, be specifically related to a kind of method utilizing EMS mutagenesis to produce watermelon mutant method to create watermelon mutants with rich variation. Background technique Watermelon (Citrullus lanatus) belongs to the cucurbitaceae watermelon genus, is a kind of important worldwide economic crop, accounts for about 7% of the world vegetable production area. my country is the world's largest watermelon producer and consumer, according to the China Agricultural Statistics Yearbook. According to statistics, in 2016, the sown area of ​​watermelon in my country reached 1.8908 million hectares, with an output of 79.4 million tons, accounting for about 70% of the world's watermelon production. Around, watermelon production occupies a very important place in horticultural crops. my country's watermelon industry generally ha...

AI Technical Summary

Problems solved by technology

Due to the ability to quickly create dominant mutants, many researchers have tried on other crops, but there have been no successful applications. On the one hand, it is difficult to collect pollen from most crops; on the other hand, paraffin oil is harmful to corn female flower silk It will not cause damage and can bear fruit normally, while paraffin oil will seriously damage the female flowers of watermelon, cucumber and other crops, resulting in no fruit setting or a very low fruit setting rate

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