A kind of polyploid induction method of mat grass

Abstract

The invention relates to the field of plant breeding, in particular to a polyploid induction method of juncus effusus. The method is characterized in that in the process of mutagenesis, a sterile explant is selected, pretreated with a high-concentration mutagenic agent firstly and then transferred to a proliferation medium containing a low-concentration mutagenic agent for culture, and mutagenic test-tube seedlings are obtained in the shortest time. The method has the advantages of being good in stability, convenient to operate and high in mutation efficiency.

Description

technical field

[0001] The invention relates to the field of plant breeding, in particular to a polyploid induction method of mat grass. Background technique

[0002] Mat grass (Juncus effusus L.) is one of the excellent natural green plant fibers, so it is suitable for weaving. Various products woven with mat grass have the functions of ventilation, moisture absorption and cooling, especially the stem of mat grass has the function of regulating dryness and humidity. function. It can keep moderately dry in summer, making people's skin feel very comfortable, and has good thermal insulation performance in winter. Therefore, mat woven fabrics have become an indispensable household daily necessities in people's daily life, and are also a unique indoor fashion decoration. Consumers love it. Although there are all kinds of plastic, chemical fiber, bamboo and other woven products in various designs and colors, the many characteristics and advantages of mat straw woven products ca...

Examples

Embodiment 1

[0036] 1. Polyploid mutagenesis and multiplication and rooting: take mat grass (Juncus effusus L.) aseptic seedlings, clean the agar at the root, place in a sterile empty bottle, soak in the mutagen, and the mutagen contains 0.1 mg / L, 0.5mg / L asulphin mixed solution, the treatment temperature is 4°C, 25°C, the treatment time is 30min, 60min, and the setting is repeated 3 times; the sterile vaccine is taken out and placed on sterile filter paper to absorb the water, and inoculated Put on the proliferation medium containing mutagen, the mutagen components include 0.01mg / L, 0.03mg / L, 0.05mg / L oryzalin, light culture at about 25°C for 30d, 60d, set 3 times of repetition;

[0037] 2. Polyploid identification: In order to reduce the workload of chromosome number detection, plants with obvious phenotypic variation were inoculated on rooting medium for cultivation. Then the part of the root tip of the plant containing the meristematic zone was cut, and the karyotype identification was...

Embodiment 2

[0048] Adopt optimized proliferation medium 1 / 2MS+6-BA3.5mg / L of the present invention and rooting medium 1 / 2MS+6-BA2.5mg / L+NAA0.6mg / L+IAA0.5mg / L+ paclobutrazol 3.0mg / L, it was found that the multiplication factor reached 7-10 in 30 days, and more than 70 effective seedlings were obtained per bottle, which greatly improved the cultivation efficiency and saved the production cost. Moreover, multiplication and rooting are simplified in one step, the rooting rate reaches more than 95%, and the time to form a complete mutagenic plant is only 30 days, which greatly improves the production efficiency.

technical field

[0001] The invention relates to the field of plant breeding, in particular to a polyploid induction method of mat grass. Background technique

[0002] Mat grass (Juncus effusus L.) is one of the excellent natural green plant fibers, so it is suitable for weaving. Various products woven with mat grass have the functions of ventilation, moisture absorption and cooling, especially the stem of mat grass has the function of regulating dryness and humidity. function. It can keep moderately dry in summer, making people's skin feel very comfortable, and has good thermal insulation performance in winter. Therefore, mat woven fabrics have become an indispensable household daily necessities in people's daily life, and are also a unique indoor fashion decoration. Consumers love it. Although there are all kinds of plastic, chemical fiber, bamboo and other woven products in various designs and colors, the many characteristics and advantages of mat straw woven products ca...

Examples

Embodiment 1

[0036] 1. Polyploid mutagenesis and multiplication and rooting: take mat grass (Juncus effusus L.) aseptic seedlings, clean the agar at the root, place in a sterile empty bottle, soak in the mutagen, and the mutagen contains 0.1 mg / L, 0.5mg / L asulphin mixed solution, the treatment temperature is 4°C, 25°C, the treatment time is 30min, 60min, and the setting is repeated 3 times; the sterile vaccine is taken out and placed on sterile filter paper to absorb the water, and inoculated Put on the proliferation medium containing mutagen, the mutagen components include 0.01mg / L, 0.03mg / L, 0.05mg / L oryzalin, light culture at about 25°C for 30d, 60d, set 3 times of repetition;

[0037] 2. Polyploid identification: In order to reduce the workload of chromosome number detection, plants with obvious phenotypic variation were inoculated on rooting medium for cultivation. Then the part of the root tip of the plant containing the meristematic zone was cut, and the karyotype identification was...

Embodiment 2

[0048] Adopt optimized proliferation medium 1 / 2MS+6-BA3.5mg / L of the present invention and rooting medium 1 / 2MS+6-BA2.5mg / L+NAA0.6mg / L+IAA0.5mg / L+ paclobutrazol 3.0mg / L, it was found that the multiplication factor reached 7-10 in 30 days, and more than 70 effective seedlings were obtained per bottle, which greatly improved the cultivation efficiency and saved the production cost. Moreover, multiplication and rooting are simplified in one step, the rooting rate reaches more than 95%, and the time to form a complete mutagenic plant is only 30 days, which greatly improves the production efficiency.

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