A kind of polyploid induction method of mat grass
A technology of polyploidy and mat grass, applied in horticultural methods, botanical equipment and methods, plant regeneration, etc., can solve the problems of low mutagenesis rate of polyploidy, poor stem toughness, and species degradation, so as to save mutagenesis Effects of exploration time, long topgrass, and short recovery period
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Embodiment 1
[0036] 1. Polyploid mutagenesis and multiplication and rooting: take mat grass (Juncus effusus L.) aseptic seedlings, clean the agar at the root, place in a sterile empty bottle, soak in the mutagen, and the mutagen contains 0.1 mg / L, 0.5mg / L asulphin mixed solution, the treatment temperature is 4°C, 25°C, the treatment time is 30min, 60min, and the setting is repeated 3 times; the sterile vaccine is taken out and placed on sterile filter paper to absorb the water, and inoculated Put on the proliferation medium containing mutagen, the mutagen components include 0.01mg / L, 0.03mg / L, 0.05mg / L oryzalin, light culture at about 25°C for 30d, 60d, set 3 times of repetition;
[0037] 2. Polyploid identification: In order to reduce the workload of chromosome number detection, plants with obvious phenotypic variation were inoculated on rooting medium for cultivation. Then the part of the root tip of the plant containing the meristematic zone was cut, and the karyotype identification was...
Embodiment 2
[0048] Adopt optimized proliferation medium 1 / 2MS+6-BA3.5mg / L of the present invention and rooting medium 1 / 2MS+6-BA2.5mg / L+NAA0.6mg / L+IAA0.5mg / L+ paclobutrazol 3.0mg / L, it was found that the multiplication factor reached 7-10 in 30 days, and more than 70 effective seedlings were obtained per bottle, which greatly improved the cultivation efficiency and saved the production cost. Moreover, multiplication and rooting are simplified in one step, the rooting rate reaches more than 95%, and the time to form a complete mutagenic plant is only 30 days, which greatly improves the production efficiency.
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