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Tissue culture method for ipomoea holubii

A Helu morning glory and tissue culture technology, applied in horticultural methods, botanical equipment and methods, plant regeneration, etc., can solve problems such as no successful examples, and achieve the effect of overcoming the scarcity of female parents and the slowness of conventional breeding methods

Inactive Publication Date: 2016-12-21
ZHEJIANG ACADEMY OF AGRICULTURE SCIENCES
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, before this, there was no report on the tissue culture research of Helu petunias, let alone any successful examples

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0028] The invention provides a method for Helu petunias tissue culture, the steps are:

[0029] 1), the preparation of culture medium

[0030] (1) Basic medium: WPM medium, in which sucrose 20-30g / L, agar 6-9g / L, pH=5.8;

[0031] (2) Starting medium: WPM+6-BA 0.2~0.5mg / L+NAA 0~0.02mg / L+0.1~0.3% activated carbon;

[0032] (3) Proliferation medium: WPM+6-BA 0.1~0.5mg / L+0.1~0.3% activated carbon;

[0033] (4) Rooting medium: WPM+IBA 0.1~0.3mg / L+0.1~0.3% activated carbon;

[0034] 2), selection and disinfection of explants

[0035] Select the stem section of Helu morning glory as the explant material, first soak it in saturated washing powder solution for 30 minutes, then rinse it with tap water, and then wash it in an alcohol solution with a volume ratio of 70% and sodium hypochlorite with an effective chlorine saturation of 1%. Soak in the solution for 40s and 7min respectively, shake several times during the period, and finally rinse with sterile water 3 to 5 times, 1min e...

Embodiment 2

[0044] The invention provides a method for Helu petunias tissue culture, the steps are:

[0045] 1), the preparation of culture medium

[0046] (1) Basic medium: WPM medium, wherein sucrose 30g / L, agar 8g / L, pH=5.8;

[0047] (2) Start medium: WPM+6-BA 0.3mg / L+NAA 0.01mg / L+0.1% activated carbon;

[0048] (3) Proliferation medium: WPM+6-BA 0.2mg / L+0.1% activated carbon;

[0049] (4) Rooting medium: WPM+IBA 0.2mg / L+0.1% activated carbon;

[0050] 2), selection and disinfection of explants

[0051] Select the stem section of Helu morning glory as the explant material, first soak it in saturated washing powder solution for 30 minutes, then rinse it with tap water, and then wash it in an alcohol solution with a volume ratio of 70% and sodium hypochlorite with an effective chlorine saturation of 1%. Soak in the solution for 40s and 7min respectively, shake several times during the period, and finally rinse with sterile water 4 times, each time for 1min;

[0052] 3), induction of...

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PUM

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Abstract

The invention discloses a tissue culture method for ipomoea holubii, which particularly includes the steps of: 1) preparation of a culture medium which includes components of a basic culture medium and culture mediums in all steps of tissue culture; 2) selection and disinfection of an explant; 3) induction of axillary buds; 4) proliferation of adventitious buds; and 5) rooting and transplanting. The method has the following advantageous effects: through plant tissue cultivation, induction of axillary buds and proliferation of adventitious buds clusters are carried out to stem fragments of the ipomoea holubii, so that a large quantity of high-quality seedlings having consistent genetic characters can be obtained within short time. The method also overcomes defects of scarcity of female parents and low speed of common reproductive methods, and also has positive significance on protection of germplasm resources of wild ipomoea holubii and promotion of seedling production in large scale.

Description

technical field [0001] The invention belongs to the field of tissue culture methods, in particular to a method for Helu petunias tissue culture. Background technique [0002] Ipomoea holubii is a plant of the genus Ipomoea in the family Convolvulaceae, native to Botswana and Namibia. Helu Morning Glory has large bulbous roots with a petrified yellow-brown surface. Its leaves are slender like willow leaves and have dark pink trumpet-shaped flowers. It has high ornamental value and is a precious succulent plant. However, under natural conditions, the Helu morning glory grows slowly, has a low reproductive rate and a small number. In order to protect wild Helu petunias from predatory collection and even extinction, the International Organization for Succulent Plant Study has listed Helu petunias in the Convention on International Trade in Endangered Species of Wild Fauna and Flora (CITES Convention) appendix, are endangered wild plants. This makes its market price remain hig...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A01H4/00
CPCA01H4/008
Inventor 吕永平王燕汪一婷牟豪杰陈志陈剑平
Owner ZHEJIANG ACADEMY OF AGRICULTURE SCIENCES
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