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A kind of Qiongdao poplar tissue culture fast propagation method

A technology of Qiongdao and rapid propagation, applied in the field of plant tissue culture, can solve problems such as slow cultivation of poplar seedlings in Qiongdao, and achieve the effects of rapid induction of axillary buds, high transplant survival rate and low cost of seedling raising.

Active Publication Date: 2022-08-09
海南省林业科学研究院
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  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

[0004] The present invention intends to provide a method for tissue culture and rapid propagation of Populus qiongdao, which solves the problem of slow cultivation of poplar qiongdao seedlings

Method used

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  • A kind of Qiongdao poplar tissue culture fast propagation method
  • A kind of Qiongdao poplar tissue culture fast propagation method
  • A kind of Qiongdao poplar tissue culture fast propagation method

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Embodiment

[0029] as attached figure 1 and figure 2 Shown: a kind of Qiongdao poplar tissue culture rapid propagation method, comprising the following steps:

[0030] S102, the weather is fine and the new branches of the perennial Qiongdao poplar mother tree are cut off within 9:00-10:00 in the morning, and the stem segments with a length of 5-10cm below the shoot tips of the new branches and full axillary buds without pests and diseases are used as explants. spare.

[0031] S104. Wash the explants in S102 with tap water for 15 minutes, then place them in a sterile ultra-clean bench and wash them with 75% alcohol for 30s in turn, rinse them with sterile water for 3-4 times, and then wash and sterilize the explants. The explants were placed in HgCl supplemented with 1-2 drops of 0.1% Tween 80 2 Soak in solution for 12-13min, in which HgCl 2 The concentration of the solution is 1.5%, and finally rinse with sterile water 4 to 5 times.

[0032] S106, as attached figure 1 As shown, the...

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Abstract

The patent of the present invention discloses a tissue culture rapid propagation method of Populus qiongdao, and specifically relates to the technical field of plant tissue culture. A method for tissue culture and rapid propagation of Populus qiongdao, comprising the following steps: cutting off new branches of perennial Populus qiongdao, cutting out stem segments of the new branches as explants; cleaning and sterilizing the explants; connecting the explants to induce Cultivate in bud medium; transfer the obtained axillary bud stem segments to proliferation medium for proliferation, cut the adventitious buds obtained by proliferation into small bud blocks, transfer the small bud blocks to the medium for cultivation and obtain a large number of clustered buds or Adventitious buds; cut the tender stems from the clump buds, and transfer them into the rooting medium, under natural light and constant temperature conditions to induce the adventitious buds to take root, the rooted seedlings obtained are moved to the greenhouse for hardening, and the tissue cultured seedlings are transplanted to Cultivation in mixed substrates can get Qiongdao poplar seedlings. The technical solution of the invention solves the problem of slow cultivation of Populus qiongdao seedlings, and can be used for rapidly and massively cultivating poplar seedlings of Qiongdao.

Description

technical field [0001] The invention relates to the technical field of plant tissue culture, in particular to a tissue culture rapid propagation method of Populus qiongdao. Background technique [0002] Qiongdaoensis (Populus qiongdaoensis T.Hong et P.Luo) is a large deciduous tree in the Salicaceae, Populus genus, hi light, fast-growing, and strong germination. The wood is suitable for pulp, wood wool, rayon, fiberboard, plywood, matchsticks , match boxes, toothpicks and other raw materials, especially suitable for food (eggs, meat) and clothes packaging boxes, but also for construction, beverage barrels, utensils, etc., with important economic value. Qiongdao poplar is an endemic tree species in Hainan, and is listed on the provincial key protected terrestrial wild plant list (2006). Currently, it is only found in Zhizailing, Baisha County and Bawangling, Changjiang County, Hainan Island. It is an endangered tree species. [0003] At present, domestic and foreign research...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): A01H4/00
CPCA01H4/001A01H4/008Y02P60/40
Inventor 陈彧邢文婷董晓娜徐佩玲陈显臻韩豫饶丹丹
Owner 海南省林业科学研究院
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