A kind of Qiongdao poplar tissue culture fast propagation method
What is Al technical title?
Al technical title is built by PatSnap Al team. It summarizes the technical point description of the patent document.
A technology of Qiongdao and rapid propagation, applied in the field of plant tissue culture, can solve problems such as slow cultivation of poplar seedlings in Qiongdao, and achieve the effects of rapid induction of axillary buds, high transplant survival rate and low cost of seedling raising.
Active Publication Date: 2022-08-09
海南省林业科学研究院
View PDF2 Cites 0 Cited by
Summary
Abstract
Description
Claims
Application Information
AI Technical Summary
This helps you quickly interpret patents by identifying the three key elements:
Problems solved by technology
Method used
Benefits of technology
Problems solved by technology
[0004] The present invention intends to provide a method for tissue culture and rapid propagation of Populus qiongdao, which solves the problem of slow cultivation of poplar qiongdao seedlings
Method used
the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
View more
Image
Smart Image Click on the blue labels to locate them in the text.
Viewing Examples
Smart Image
Click on the blue label to locate the original text in one second.
Reading with bidirectional positioning of images and text.
Smart Image
Examples
Experimental program
Comparison scheme
Effect test
Embodiment
[0029] as attached figure 1 and figure 2 Shown: a kind of Qiongdao poplar tissue culture rapid propagation method, comprising the following steps:
[0030] S102, the weather is fine and the new branches of the perennial Qiongdao poplar mother tree are cut off within 9:00-10:00 in the morning, and the stem segments with a length of 5-10cm below the shoot tips of the new branches and full axillary buds without pests and diseases are used as explants. spare.
[0031] S104. Wash the explants in S102 with tap water for 15 minutes, then place them in a sterile ultra-clean bench and wash them with 75% alcohol for 30s in turn, rinse them with sterile water for 3-4 times, and then wash and sterilize the explants. The explants were placed in HgCl supplemented with 1-2 drops of 0.1% Tween 80 2 Soak in solution for 12-13min, in which HgCl 2 The concentration of the solution is 1.5%, and finally rinse with sterile water 4 to 5 times.
[0032] S106, as attached figure 1 As shown, the...
the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to view more
PUM
Login to view more
Abstract
The patent of the present invention discloses a tissue culture rapid propagation method of Populus qiongdao, and specifically relates to the technical field of plant tissue culture. A method for tissue culture and rapid propagation of Populus qiongdao, comprising the following steps: cutting off new branches of perennial Populus qiongdao, cutting out stem segments of the new branches as explants; cleaning and sterilizing the explants; connecting the explants to induce Cultivate in bud medium; transfer the obtained axillary bud stem segments to proliferation medium for proliferation, cut the adventitious buds obtained by proliferation into small bud blocks, transfer the small bud blocks to the medium for cultivation and obtain a large number of clustered buds or Adventitious buds; cut the tender stems from the clump buds, and transfer them into the rooting medium, under natural light and constant temperature conditions to induce the adventitious buds to take root, the rooted seedlings obtained are moved to the greenhouse for hardening, and the tissue cultured seedlings are transplanted to Cultivation in mixed substrates can get Qiongdao poplar seedlings. The technical solution of the invention solves the problem of slow cultivation of Populus qiongdao seedlings, and can be used for rapidly and massively cultivating poplar seedlings of Qiongdao.
Description
technical field [0001] The invention relates to the technical field of plant tissue culture, in particular to a tissue culture rapid propagation method of Populus qiongdao. Background technique [0002] Qiongdaoensis (Populus qiongdaoensis T.Hong et P.Luo) is a large deciduous tree in the Salicaceae, Populus genus, hi light, fast-growing, and strong germination. The wood is suitable for pulp, wood wool, rayon, fiberboard, plywood, matchsticks , match boxes, toothpicks and other raw materials, especially suitable for food (eggs, meat) and clothes packaging boxes, but also for construction, beverage barrels, utensils, etc., with important economic value. Qiongdao poplar is an endemic tree species in Hainan, and is listed on the provincial key protected terrestrial wild plant list (2006). Currently, it is only found in Zhizailing, Baisha County and Bawangling, Changjiang County, Hainan Island. It is an endangered tree species. [0003] At present, domestic and foreign research...
Claims
the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to view more
Application Information
Patent Timeline
Application Date:The date an application was filed.
Publication Date:The date a patent or application was officially published.
First Publication Date:The earliest publication date of a patent with the same application number.
Issue Date:Publication date of the patent grant document.
PCT Entry Date:The Entry date of PCT National Phase.
Estimated Expiry Date:The statutory expiry date of a patent right according to the Patent Law, and it is the longest term of protection that the patent right can achieve without the termination of the patent right due to other reasons(Term extension factor has been taken into account ).
Invalid Date:Actual expiry date is based on effective date or publication date of legal transaction data of invalid patent.
Login to view more 
Login to view more