Kit and method for determining uric acid

Abstract

The invention relates to a kit and method for determining uric acid. The kit comprises a reagent set 1 and a reagent set 2, wherein the reagent set 1 comprises a chromogen; the reagent set 2 comprises 4-aminoantipyrine, peroxidase and uricase; and the chromogen is or comprises N-ethyl-N-(2-hydroxy-3-sulfopropyl)-3,5-dimethoxyaniline. The kit and method can lower the interference caused by calcium phenolsulfonate and / or etamsylate in the sample in the uric acid detection process.

Description

technical field [0001] The invention relates to the technical field of medical testing. In particular, the present invention relates to a kit and method for detecting uric acid, and more particularly to a kit for measuring uric acid mainly used in automatic biochemical analyzers. Background technique [0002] Uric acid (Uric Acid, UA) is the final product of the metabolism of purine compounds in the body, which is weakly acidic. Uric acid can come from the body or from the catabolism of purines in food. In the body, uric acid is mainly produced in the liver, a small part can be excreted through the liver with bile, and most of the rest are excreted from the kidneys. The solubility of uric acid is small, so it is easy to precipitate crystals when the concentration in the body is high. Uric acid is considered to be closely related to the occurrence and development of gout, cardiovascular and cerebrovascular diseases, metabolic syndrome, ureteral stones and kidney diseases. ...

AI Technical Summary

Problems solved by technology

[0008] The interference of calcium dobesilate and phenetamine on the enzymatic detection of uric acid caused great inconvenience to patients who were administered these two drugs

For example, hyperuricemia has a direct impact on diabetes, hypertriglyceridemia, metabolic syndrome, coronary heart disease, and kidney damage. For diabetic patients, the administered calcium dobesilate has no effect on the enzymatic detection of uric acid. The interference of uric acid may cause incorrect assessment of uric acid, thereby masking and delaying the disease

For surgical patients, the interference of the administered uric acid with enzymatic detection of uric acid may make it impossible to correctly assess the uric acid level, resulting in postoperative complications and affecting the prognosis of patients

Therefore, this interference seriously affects the accuracy of the test results, affects the correct judgment of clinicians, and ultimately delays the treatment of patients, or causes casualties to patients by adopting unreasonable treatment measures

[0009] At present, the enzymatic detection kits sold on the market generally have the defect that they cannot effectively resist the interference of calcium dobesilate and phensulfonate

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