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A method for high-density cultivation of Artemia under the environment of algae cultivation

A high-density, artemia technology, applied in animal husbandry, etc., can solve the problems of slow growth of artemia, achieve high survival rate, shorten spawning rate, stabilize water environment and survival rate

Active Publication Date: 2019-10-29
TIANJIN OCEAN PAL CAROL BIOTECH +1
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0003] In view of this, the present invention aims to propose a method for high-density cultivation of Artemia in an algae-raising environment. This method not only realizes the growth of algae, but also provides oxygen for Artemia at high density while carrying out photosynthesis and oxygen production. The amount provides guarantee, solves the problem of slow growth of Artemia due to insufficient bait, and at the same time ensures that Artemia has a high survival rate, and the present invention is also suitable for ensuring a stable survival rate in large breeding sites

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0026] Use a 20L transparent glass tank to add 10L algae liquid with a density of 2,000,000 / ml for aeration, then add 100,000 Artemia nauplii, and add algae culture medium according to the ratio of f / 2 medium to the amount of 10L algae. Add the medium of algae according to the amount of 5L of algae on the third day, and add the culture medium according to the amount of 5L of algae on the sixth day. Add 25ml at four o'clock in the afternoon every day with a concentration of 10 9 Pseudomonas aeruginosa per mL, added once every 24 hours, the salinity is 30g dissolved salt / L, the pH fluctuates between 8.5 and 9.6, the concentration of dissolved oxygen is greater than 6mg / L, and the water temperature is 26±2°C ;

[0027] The configuration process of the algae solution is to add f / 2 medium into the sterile seawater, then inoculate the algae species into the sterile seawater with f / 2 medium on the ultra-clean workbench, and culture them in the incubator; When the density of algae i...

Embodiment 2

[0032] Use a 20L transparent glass tank to add 10L of algae liquid with a density of 2,000,000 / ml for aeration, then add 100,000 Artemia nauplii, and add algae medium according to the amount of 10L of algae, and add algae according to the amount of 5L of algae on the third day On the 6th day, also add culture medium according to 5L algae. Add 25ml of Pseudomonas aeruginosa every night. The algae solution is prepared by mixing brine with tap water. The salinity is 30g dissolved salt / L, the pH fluctuates between 8.5 and 9.6, and the concentration of dissolved oxygen is greater than 6mg / L. The water temperature is 26±2℃;

[0033] The configuration process of the algae solution is to add f / 2 medium into the sterile seawater, then inoculate the algae species into the sterile seawater with f / 2 medium on the ultra-clean workbench, and culture them in the incubator; When the density of algae is 2,000,000 / ml, pour the algae solution into the culture tank, and add air stones for aerati...

Embodiment 3

[0038] Use a 75L transparent plastic bucket to add 50L of algae liquid with a density of 2,000,000 / ml for aeration, then add 500,000 Artemia nauplii, and add algae medium according to the amount of 50L of algae, and add algae according to the amount of 25L of algae on the third day On the 6th day, 25L of algae was added to the culture medium for cultivation. Add 125ml of Pseudomonas aeruginosa every night. The algae solution is prepared by mixing brine with tap water. The salinity is 30g dissolved salt / L, the pH fluctuates between 8.5 and 9.6, and the concentration of dissolved oxygen is greater than 6mg / L. The water temperature is 26±2℃;

[0039] The algae is Thalassiosira; and the eggs of Artemia Bohai Bay are used.

[0040] The configuration process of the algae solution is to add f / 2 medium into the sterile seawater, then inoculate the algae species into the sterile seawater with f / 2 medium on the ultra-clean workbench, and culture them in the incubator; When the density...

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Abstract

The invention provides a method of breeding artemia in high density under alga cultivation environment. In an artemia cultivation water environment, the density of algae is one million cells / mL to ten million cells / mL; the water environment contains pseudomonas aeruginosa whose concentration is 10<6> to 10<8> / mL; the density of artemia is 5 to 12 / mL; and the optimized density of artemia is 5 to 10 / mL. The method increases dissolved oxygen in water and not only solves the problem of initial feed of artemia, but also solves the problem of low survival rate and spawning rate of high density cultivation. The method is also suitable in large aquaculture pond for guaranteeing stable water environment and survival rate.

Description

technical field [0001] The invention belongs to the technical field of high-density cultivation of Artemia, in particular to a method for high-density cultivation of Artemia in an algal culture environment. Background technique [0002] Artemia is also known as "brine shrimp". Large artemia is generally the preferred bait for shrimp, crab, seahorse and sea cucumber. There is generally no live feed such as artemia in the market, only the frozen body of artemia, and the hatched nauplii become large artemia after a short week, which not only solves the problems of high density of artemia, low survival rate and long breeding time, It also solves the economic cost of buying frozen large artemia and the problem that the frozen large artemia is not fresh. But at present, people have different opinions on the bait needed for high-density culture of Artemia. Therefore, no one has proposed the impact of different baits on high-density culture of Artemia. The growth status of Artemia ...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): A01K67/033
CPCA01K67/033
Inventor 柴英辉孙朦朦于宏周文礼李连星王永苓
Owner TIANJIN OCEAN PAL CAROL BIOTECH
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