Porcine epidemic diarrhea virus and separation culture method

A porcine epidemic diarrhea, isolation and cultivation technology, applied in the field of new porcine epidemic diarrhea virus and its isolation and cultivation, can solve the problems of unsuccessful attempts, difficult propagation of PEDV, and difficulty in isolation of PEDV
CN106399259AInactive Publication Date: 2017-02-15BEIJING DAWEIJIA BIOTECH SHARE CO LTD
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Patent Information

Authority / Receiving Office
CN · China
Patent Type
Applications(China)
Current Assignee / Owner
BEIJING DAWEIJIA BIOTECH SHARE CO LTD
Publication Date
2017-02-15
Estimated Expiration
Not applicable · inactive patent

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Abstract

The present invention discloses a strain of porcine epidemic diarrhea virus and a separation culture method. According to the present invention, the virus is Coronaviridae Coronavirus porcine epidemic diarrhea virus, is named PEDV / CH / BJ / 2014, and has the preservation number of CGMCC No.10111; the culture of the virus requires a blank DMEM culture liquid containing trypsin; and the porcine epidemic diarrhea virus has good immunogenicity, is the current domestic epidemic variant, can be used for preparing PEDV diagnosis kit, PEDV attenuated vaccines and inactivated vaccines, and enriches the existing PEDV vaccine resources.
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Description

technical field

[0001] The invention relates to the field of microbes, in particular to a new strain of porcine epidemic diarrhea virus and a method for its isolation and cultivation. Background technique

[0002] Porcine Epidemic Diarrhea Virus (PEDV) is a member of the Coronaviridae family of Coronaviridae, which can cause intestinal infectious diseases of pigs characterized by vomiting, diarrhea and dehydration. Pigs of all ages are susceptible, but suckling piglets are the most affected, with mortality rates as high as 100%. In recent years, the outbreak of PED in many provinces in my country has caused serious economic losses to the pig industry.

[0003] The prevention and control of porcine epidemic diarrhea disease is mainly based on vaccination. At present, there is no specific drug to treat this disease, and the vaccine used in China is mainly PEDV CV777 strain. This strain was isolated earlier. Compared with the new mutant strain Lack of extensive immunogenicity...

Claims

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