Greenhouse seedling stage inoculation identification method of disease-resistance strength of resisting to alternaria alternata of tobacco

Abstract

The invention provides a greenhouse seedling stage inoculation identification method of disease-resistance strength of resisting to alternaria alternata of tobacco. The method comprises the following steps: I, cultivating tobacco alternaria alternata germs on a PDA plate medium, and preparing inoculation fluid; II, transplanting tobacco into a flowerpot; and III, spraying the inoculation fluid to plants after the growth of tobacco true leaves and conducting inoculating, conducting cultivation and identifying the disease-resistance strength. According to the greenhouse seedling stage inoculation identification method of the disease-resistance strength of resisting to the alternaria alternata of tobacco, the scientific and reasonable identification method and process parameter control are set in accordance with researches on infection processes and infection parameters of the alternaria alternata; and meanwhile, in combination with the growth properties of the tobacco plants as well as local disease resistance of the plants, disease-resistance strength of resisting to the alternaria alternata of tobacco is effectively and reasonably identified

Description

technical field [0001] The invention relates to the field of microbial control, in particular to a method for identifying the strength and weakness of tobacco resistance against red spot disease in a greenhouse at seedling stage. Background technique [0002] Tobacco red spot disease is one of the main diseases of tobacco production in my country. The disease occurs in various tobacco-producing areas in my country, and it can infect leaves, stems, pedicels and capsules. Leaf infection mostly occurs from the lower leaves and gradually develops upwards. Lesions are small yellowish-brown spots at first, and later develop into brown round or nearly round spots with reddish brown or dark brown concentric ring patterns. When the expansion is fast, a yellow halo appears on the edge. When the humidity is high, a dark brown or black mold layer can be seen on the spots. Lesions are brittle and easily broken. In severe cases, the lesions merge and the leaves become broken leaves. ...

AI Technical Summary

Problems solved by technology

[0003] According to the occurrence rule of red spot disease, (initial infection, transmission route, re-infection, etc.), the prevention and control of red spot disease should adopt comprehensive control measures that combine planting of disease-resistant varieties, chemical control and implementation of reasonable cultivation measures. How to screen out disease-resistant varieties is the key. In traditional screening, there is no unified screening method for the screening of red spot disease-resistant varieties, resulting in inconsistencies in screening methods and differences in screening effects.