Greenhouse seedling stage inoculation identification method of disease-resistance strength of resisting to alternaria alternata of tobacco

An identification method and disease resistance technology, which is applied in the field of identification of tobacco red spot disease resistance and inoculation at the greenhouse seedling stage, can solve the problems of lack of a relatively unified screening method, differences in screening effects, and non-uniform screening methods, etc. To achieve the effect of accurate and reliable identification results and reasonable methods

Inactive Publication Date: 2017-02-15
GUANGDONG BRANCH OF CHINA TOBACCO GENERAL
View PDF1 Cites 4 Cited by
  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0003] According to the occurrence rule of red spot disease, (initial infection, transmission route, re-infection, etc.), the prevention and control of red spot disease should adopt comprehensive control measures that combine planting of disease-resistant varieties, chemical control and implementation of reasonable cultivation measures. How to screen out disease-resistant varieties is the key. In traditional screening, there is no unified screening method for the screening of red spot disease-resistant varieties, resulting in inconsistencies in screening methods and differences in screening effects.

Method used

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
View more

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0025] (Guangdong) Inoculation identification of red spot disease resistance at seedling stage in greenhouse

[0026] 1. Preparation of Inoculum

[0027] Tobacco Alternaria was cultured on PDA plate medium (potato dextrose agar medium) at 26°C for 7 days, and the concentration was 1*10 with 1% glucose solution. 5 The spore suspension in CFU / ml was used as the inoculum.

[0028] 2. Preparation of Inoculation Material

[0029] Transplant the tobacco into flower pots with a diameter greater than 10 cm, 20 pots per species, 2 plants per pot, and at least 3 repetitions.

[0030] 3. Inoculation method

[0031] When the tobacco seedlings have 9-10 true leaves, red spot disease spores (1*10 5 CFU / ml) inoculation, the spore suspension is sprayed on the plant with a small hand-held sprayer, then placed in an artificial climate box, the set temperature is 26-30 ° C, the relative humidity is 70%, and the susceptible varieties and disease-resistant varieties are used as controls , as ...

Embodiment 2

[0036] Embodiment 2-3 method is the same as embodiment 1, and difference is that embodiment 2 is carried out in Shandong, and embodiment 3 is carried out in Yunnan.

[0037] The identification results show that the disease resistance of Guangdong, Yunnan, and Shandong is not much different (the severity of the disease in the greenhouse is not much different), and the data are as follows:

[0038] 7d(％) 10d(％) 20d(％) Disease severity (level) Example 1 1 2 4 3 Example 2 1 3 8 5 Example 3 1 2 5 3

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to view more

PUM

PropertyMeasurementUnit
diameteraaaaaaaaaa
Login to view more

Abstract

The invention provides a greenhouse seedling stage inoculation identification method of disease-resistance strength of resisting to alternaria alternata of tobacco. The method comprises the following steps: I, cultivating tobacco alternaria alternata germs on a PDA plate medium, and preparing inoculation fluid; II, transplanting tobacco into a flowerpot; and III, spraying the inoculation fluid to plants after the growth of tobacco true leaves and conducting inoculating, conducting cultivation and identifying the disease-resistance strength. According to the greenhouse seedling stage inoculation identification method of the disease-resistance strength of resisting to the alternaria alternata of tobacco, the scientific and reasonable identification method and process parameter control are set in accordance with researches on infection processes and infection parameters of the alternaria alternata; and meanwhile, in combination with the growth properties of the tobacco plants as well as local disease resistance of the plants, disease-resistance strength of resisting to the alternaria alternata of tobacco is effectively and reasonably identified

Description

technical field [0001] The invention relates to the field of microbial control, in particular to a method for identifying the strength and weakness of tobacco resistance against red spot disease in a greenhouse at seedling stage. Background technique [0002] Tobacco red spot disease is one of the main diseases of tobacco production in my country. The disease occurs in various tobacco-producing areas in my country, and it can infect leaves, stems, pedicels and capsules. Leaf infection mostly occurs from the lower leaves and gradually develops upwards. Lesions are small yellowish-brown spots at first, and later develop into brown round or nearly round spots with reddish brown or dark brown concentric ring patterns. When the expansion is fast, a yellow halo appears on the edge. When the humidity is high, a dark brown or black mold layer can be seen on the spots. Lesions are brittle and easily broken. In severe cases, the lesions merge and the leaves become broken leaves. ...

Claims

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to view more

Application Information

Patent Timeline
no application Login to view more
Patent Type & Authority Applications(China)
IPC IPC(8): C12Q1/18C12R1/645
CPCC12Q1/18
Inventor 陈泽鹏
Owner GUANGDONG BRANCH OF CHINA TOBACCO GENERAL
Who we serve
  • R&D Engineer
  • R&D Manager
  • IP Professional
Why Eureka
  • Industry Leading Data Capabilities
  • Powerful AI technology
  • Patent DNA Extraction
Social media
Try Eureka
PatSnap group products

Browse by: Latest US Patents, China's latest patents, Technical Efficacy Thesaurus, Application Domain, Technology Topic.

© 2024 PatSnap. All rights reserved.Legal|Privacy policy|Modern Slavery Act Transparency Statement|Sitemap