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Coccidiosis vaccine component having cross immunological protection capacity and vaccine

A technology of cross-immunity and coccidiosis, applied in vaccines, veterinary vaccines, drug combinations, etc.

Active Publication Date: 2017-02-22
CHINA AGRI UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0004] In order to solve the risk that the existing coccidiosis vaccine components do not have cross-immune protection and the resulting coccidiosis vaccination brings to the aquaculture industry, the purpose of the present invention is to construct a class of worm species with cross-immune protection as a coccidiosis vaccine. Insect disease vaccine components, that is, using transgenic technology to construct transgenic strains expressing single or multiple immunodominant antigens of other insect species

Method used

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  • Coccidiosis vaccine component having cross immunological protection capacity and vaccine
  • Coccidiosis vaccine component having cross immunological protection capacity and vaccine
  • Coccidiosis vaccine component having cross immunological protection capacity and vaccine

Examples

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Embodiment 1

[0048] In this example, Eimeria tenella (Eimeriatenella), one of the most serious coccidia species that endangers the poultry industry, is used as an expression vector, and the strategy of the present invention is used to express the immunodominant antigen IMP1 of Eimeria maxima ( Immune mapped protein 1, IMP1). To evaluate its immunogenicity and immune protection against Eimeria maxima infection. Measuring indicators were oocyst excretion, flock weight gain and intestinal lesion score.

[0049] The construction of the novel vaccine components is the use of restriction endonuclease-mediated nucleofection technology to transform the transfection vector ( figure 1A) After the coccidia sporozoites were transfected, chicks were inoculated in the cloaca to obtain transgenic coccidia oocysts (Trans 1), and a large number of transgenic coccidia populations were obtained through continuous drug screening and flow sorting. The new vaccine component for immunization is a clonal popula...

Embodiment 2

[0059] In this example, on the basis of the existing novel vaccine component (Trans 1 described in Example 1), the immunodominant antigen AMA1 (Apical membrane antigen 1, AMA1) of Eimeria maxima was additionally expressed. A novel vaccine component (Trans 2) expressing two immunodominant antigens was constructed. The verification of its own biological characteristics and immunogenicity was carried out according to the description in Example 1, and there was no significant change. The amount of oocysts excreted after attacking the worms, the weight gain of the chicken population and the lesion degree of the middle section of the small intestine are used to reflect the immune protection of the novel vaccine components of the present invention against the infection of heterogeneous strains, which are described in detail as follows:

[0060] 1. Using oocyst excretion as an index to measure the protective power of the new vaccine components against heterogeneous strains ( Figure ...

Embodiment 3

[0064] In this embodiment, on the basis of existing novel vaccine components (Trans 2 described in Example 2), the immunodominant antigen Profilin of Eimeria maxima was expressed ( Figure 5 A). A novel vaccine component (Trans 3) expressing three immunodominant antigens was constructed. The verification of its own biological characteristics and immunogenicity was carried out according to the description in Example 1, and there was no significant change. The amount of oocysts excreted after attacking the worms, the weight gain of the chicken population and the lesion degree of the middle section of the small intestine are used to reflect the immune protection of the novel vaccine components of the present invention against the infection of heterogeneous strains, which are described in detail as follows:

[0065] 1. Using oocyst excretion as an index to measure the protective power of the new vaccine components against heterogeneous strains ( Figure 5 B). It can be seen tha...

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Abstract

The invention relates to biological immunity and a vaccine, and in particular discloses a coccidiosis vaccine component having a cross immunological protection capacity and a vaccine containing the coccidiosis vaccine component. The coccidiosis vaccine component is a transgenic coccidiosis strain capable of expressing heterogeneous coccidiosis immunity dominant antigens. The transgenic coccidiosis strain, which simultaneously has immunogenicities of a parental coccidiosis strain and a heterogeneous coccidiosis strain, can enhance the cross immunological protection capacity of the coccidiosis vaccine component when the transgenic coccidiosis strain serves as the coccidiosis vaccine component. Based upon experimental studies, it discovers that the cross immunological protection capacity, which is provided by the novel vaccine component, is positively correlated to the quantity of the expressed heterogeneous dominant antigens; therefore, preferably, a plurality of immunity dominant antigens are expressed. According to the coccidiosis vaccine component and a construction strategy thereof provided by the invention, the immunological protection capacity can be offered for various insect infections; and the cross immunological protection capacity is universal.

Description

technical field [0001] The invention relates to biological immunity and vaccines, in particular to coccidiosis vaccine components with cross immune protection. Background technique [0002] Chicken coccidiosis is one of the diseases that must be prevented and controlled in large-scale farms, and its prevention and control measures rely on drug prevention and vaccination. However, the rapid emergence of drug resistance against coccidia, the difficulty in developing new anti-coccidia drugs, and the demand for green and healthy farming have made drug prevention and control more and more restricted. Chicken coccidiosis live oocyst vaccine is currently the only approved protozoan vaccine for the prevention and control of chicken coccidiosis. control. [0003] Coccidiosis vaccines with live oocysts as the main component cannot fully replace anticoccidial drugs for coccidiosis control mainly due to the characteristics of the vaccine species. First of all, there are 7 species of ...

Claims

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Application Information

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IPC IPC(8): A61K39/012A61P33/02A61P37/04
CPCA61K39/012A61K2039/552
Inventor 索勋汤新明刘贤勇索静霞
Owner CHINA AGRI UNIV
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