Method for fast, simply and conveniently performing bemisia tabaci adult RNA (Ribonucleic Acid) interference

Abstract

The invention discloses a method for performing bemisia tabaci RNA (Ribonucleic Acid) interference by a feeding method. The method comprises the following steps of (1) preparing bemisia tabaci feeding nutrition solution; (2) preparing an experiment device material; (3) feeding the bemisia tabaci: firstly preparing dsRNA; taking a small piece of opening sealing film to cover one end of a glass tube; then, adding the prepared nutrition solution; taking a small piece of opening sealing film to cover a liquid drip; manufacturing a small bag which contains the nutrition solution and dsRNA and is formed by the double-layer opening sealing film; releasing the bemisia tabaci into a pipe from the other end opening; plugging black cotton plug; covering a light shielding pipe sleeve; putting the glass tube into a culture box; enabling the direction of the feeding small bag towards the light source. The method has the advantages that the phototropism of the bemisia tabaci is used for creating a black environment, so that the bemisia tabaci can fast eat the feeding solution with dsRNA; the death rate of the bemisia tabaci adult is obviously reduced; high timeliness and good repeatability are realized.

Description

Technical field [0001] The invention belongs to the field of biology, and relates to a rapid and simple construction of an RNA interference method system for the adult Bemisia tabaci. Background technique [0002] Bemisia tabaci (Gennadius) is a worldwide pest that seriously damages vegetables, flowers, and horticultural crops. It has the characteristics of a wide host range, high reproduction rate, transmission of plant viruses, strong outbreaks, and resistance to pesticides. The hazards of Bemisia tabaci include direct feeding of plant sap to make plants wilt, secrete honeydew to form coal pollution, thereby inhibiting photosynthesis, and more serious is that Bemisia tabaci can transmit a variety of plant viruses, such as Bemisia tabaci can transmit plants Tomato yellow leaf curl virus (TYLCV), a gemini virus, has caused a serious reduction in tomato production or even production. . [0003] Bemisia tabaci has formed more than 30 different cryptic species in the long evolutiona...

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Problems solved by technology

For further verification, the researchers injected single-stranded RNA transcribed in vitro into nematodes, and found that the gene suppression effect was significantly weakened, but the silencing effect caused by purified double-stranded RNA was quite different, and it could specifically and efficiently inhibit the gene. Express

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