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Method for preparing compounded antihypertensive peptide through marine organisms

A blood pressure-lowering peptide, the technology of marine organisms, applied in the field of peptide preparation

Inactive Publication Date: 2017-02-22
威海红印食品有限公司
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

At the same time, at present, there are no research reports on the use of various marine organisms to prepare compound hypotensive peptides

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0021] A method for preparing compound hypotensive peptides from marine organisms, the specific process steps are as follows:

[0022] a. Selection and processing of raw materials Weigh 10kg of sea cucumber, 50kg of jellyfish, 25kg of kelp and 15kg of Chinese hairy shrimp as raw materials, soak in cold water for 36 hours, remove salt, wash, remove and drain, and use a beater respectively Perform beating treatment, then add 20 times of pure water and use a colloid mill to refine the material to obtain sea cucumber pulp, jellyfish pulp, kelp pulp and Chinese hairy shrimp pulp;

[0023] b. Enzymolysis Heat the sea cucumber pulp, jellyfish pulp, kelp pulp and Chinese hair shrimp pulp obtained in step (a) until the solution center temperature is 100° C., keep for 10 minutes, and kill microorganisms; Carry out enzymolysis treatment then, wherein the condition of each slurry enzymolysis is:

[0024] Adjust the temperature of the sea cucumber slurry to 55°C, add 90 g of compound prot...

Embodiment 2

[0033] A method for preparing compound hypotensive peptides from marine organisms, the specific process steps are as follows:

[0034] a. Selection and processing of raw materials Weigh 5kg of sea cucumber, 65kg of jellyfish, 10kg of kelp and 20kg of Chinese hairy shrimp as raw materials, soak in cold water for 48 hours, remove salt, wash, remove and drain, and use a beater respectively Perform beating treatment, then add 10 times of pure water and use a colloid mill to refine the material to obtain sea cucumber pulp, jellyfish pulp, kelp pulp and Chinese hairy shrimp pulp;

[0035] b. Enzymolysis Heat the sea cucumber pulp, jellyfish pulp, kelp pulp and Chinese hair shrimp pulp obtained in step (a) to a solution center temperature of 95° C., keep for 10 minutes, and kill microorganisms; Carry out enzymolysis treatment then, wherein the condition of each slurry enzymolysis is:

[0036] Adjust the temperature of the sea cucumber slurry to 50°C and add 25 g of compound protease...

Embodiment 3

[0045] A method for preparing compound hypotensive peptides from marine organisms, the specific process steps are as follows:

[0046] a. Selection and processing of raw materials Weigh 8kg of sea cucumber, 60kg of jellyfish, 22kg of kelp and 10kg of Chinese hairy shrimp as raw materials, soak them in cold water for 24 hours, remove the salt, wash them, remove and drain the water, and use a beater respectively Perform beating treatment, then add 30 times of pure water and use a colloid mill to refine the material to obtain sea cucumber pulp, jellyfish pulp, kelp pulp and Chinese hairy shrimp pulp;

[0047] b. Enzymolysis Heat the sea cucumber pulp, jellyfish pulp, kelp pulp and Chinese hair shrimp pulp obtained in step (a) until the solution center temperature is 100° C., keep for 10 minutes, and kill microorganisms; Carry out enzymolysis treatment then, wherein the condition of each slurry enzymolysis is:

[0048] Adjust the temperature of the sea cucumber slurry to 52°C, ad...

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Abstract

The invention relates to a method for preparing a compounded antihypertensive peptide through marine organisms. The method includes the steps that 5%-10% by weight of trepang, 50%-65% by weight of jellyfishes, 10%-25% by weight of kelp and 10%-20% by weight of acetes chinensis are weighed and serve as raw materials, and the raw materials are desalted and softened respectively; then the raw materials are pulped respectively to be watered and ground into pulp; microorganisms are removed in a heated mode; cooling is carried out, then enzymolysis treatment is carried out, and enzyme denaturing is carried out; the product is cooled to be centrifuged, liquid supernatant is taken, and macromolecules are filtered and removed through a tubular membrane; the filtered liquid is graded by intercepting an organic membrane with the molecular mass of 3000Dalton, intercepted liquid with the molecular mass below 3000Dalton is collected, subjected to nanofiltration, concentrated and desalted, dried and compounded, and the compounded antihypertensive peptide is obtained. The best optimized enzymolysis technical formula is determined in the method; as pipe filtration, ultrafiltration and nanofiltration separation and extraction technologies are adopted, the extracting efficiency is high, and the obtained compounded antihypertensive peptide is remarkable in blood-pressure reduction effect. The method is scientific in material selecting, reasonable in technology and easy and convenient to operate, and industrial production is easy to achieve.

Description

technical field [0001] The invention relates to the preparation of a peptide, in particular to a method for preparing a compound blood pressure-lowering peptide by using marine organisms. Background technique [0002] Antihypertensive peptides, also known as angiotensin converting enzyme (Angiotensin Converting Enzyme, EC3.4.15.1, ACE) inhibitory peptides, are a class of peptides isolated from food proteins that can significantly lower blood pressure. molecular peptide. Among the many antihypertensive substances, antihypertensive peptides have become a hotspot in the research of active peptides because of their non-toxic and side effect antihypertensive function. The antihypertensive peptide isolated from natural food protein, although the antihypertensive effect is not as strong as that of synthetic drugs, but it has high safety, and it only has antihypertensive effect on hypertensive patients, and has no antihypertensive effect on normal blood pressure. Excessive blood p...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12P21/06C07K1/34
CPCC12P21/06C07K1/34
Inventor 迟桂丽
Owner 威海红印食品有限公司
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