Rapid detecting mycoplasma ovipneumoniae method

Abstract

The invention relates to a rapid detecting mycoplasma ovipneumoniae method, according to the gene sequence of the detected mycoplasma ovipneumoniae designing a LAMP primer; establishing a reaction system for LAMP visualization method, and conducting optimizations for LAMP reaction conditions; gathering and embrocating the sheep nasal secretions, preparing DNA template solution, conducting detections using the optimized reaction system and reaction condition. The method targets the shortcomings of requiring professional instruments and labs and the like in detecting mycoplasma ovipneumoniae in the existing methods, which limits the promotion and application of these diagnosis methods in clinics, and establishes a rapid detecting LAMP visualization method to detect MO, the method has very high specificity and sensitivity, and provides a rapid, simple, and accurate detection method for clinical diagnoses of sheep MO infection.

Description

technical field [0001] The invention relates to the technical field of biological detection, in particular to a method for rapidly detecting ovine mycoplasma pneumoniae in a visualized manner. Background technique [0002] Mycoplasma ovis pneumonia, also known as ovine infectious pleuropneumonia, is caused by Mycoplasma ovis pneumoniae (Mycoplasma ovipneumoniae, MO) causing interstitial, atypical pneumonia, and suppurative pneumonia in sheep and goats. [0003] Since the first report in sheep by Mackay et al. in 1963, Mycoplasma ovis pneumonia has been widespread worldwide. MO not only infects sheep and goats, but also other ruminants such as bighorn sheep. In recent years, there have been reports of the occurrence and prevalence of Mycoplasma ovis pneumonia in Xinjiang, Gansu, Inner Mongolia, Qinghai, Shaanxi, Ningxia, Liaoning, Jiangsu, Sichuan, Yunnan and other provinces with developed sheep farming industry. With the healthy development of the sheep industry, the harm i...

AI Technical Summary

Problems solved by technology

Indirect ELISA has high specificity, sensitivity, stability and reproducibility, but it can only detect MO antibody and cannot directly detect pathogens

[0009] The PCR method is sensitive and fast, but it relies on expensive and complex precision instruments, making it difficult to promote and popularize it at the grassroots level with poor conditions

[0010] In addition, the above detection methods generally require professional instruments and laboratories, which limits the clinical application of these diagnostic methods

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