An automated cell expansion system

Abstract

The invention relates to the technical field of cell culture and especially relates to an automatic cell expansion system. The automatic cell expansion system realizes automatic real-time regulation and control of the amount of gas dissolved in a cell growth environment and provides a stable growth environment for cell culture. The system comprises a culture liquid delivery device and a hollow fiber reactor communicated with the culture liquid delivery device through a culture liquid circulation conduit. The hollow fiber reactor comprises a culture liquid channel and a cell growth chamber. The culture liquid delivery device comprises a gas dissolver. The gas dissolver comprises a housing, a gas inlet, a culture liquid inlet and a culture liquid outlet, wherein the gas inlet, the culture liquid inlet and the culture liquid outlet are arranged on the housing. Through the culture liquid inlet and the culture liquid outlet, the gas dissolver and the culture liquid channel form a circulation loop. The automatic cell expansion system also comprises a gas supplying device communicated with the gas inlet. A gas flow control valve is arranged between the gas supplying device and the gas inlet. The automatic cell expansion system also comprises a control system electrically connected to the gas flow control valve.

Description

technical field [0001] The invention relates to the technical field of cell culture, in particular to an automated cell expansion system. Background technique [0002] T cells have a variety of biological functions, such as directly killing target cells, assisting or inhibiting B cells to produce antibodies, responding to specific antigens and mitogens, and producing cytokines, etc. Mature T cells are distributed to the periphery through the bloodstream The thymus-dependent area of ​​the immune organ settles, and can be recycled through lymphatic vessels, peripheral blood and tissue fluid, etc., to play the functions of cellular immunity and immune regulation. It has become possible to successfully grow T cells and infuse them in large numbers into patients to boost the immune system. [0003] There are two main methods for culturing T cells in the prior art. One is to use a cell culture dish, a high-grade laminar flow hood and a carbon dioxide incubator for culturing, whic...

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Problems solved by technology

[0003] In the prior art, there are mainly two methods for culturing T cells. One is to use cell culture dishes, high-grade laminar flow hoods and carbon dioxide incubators for culturing. This method has low efficiency and cannot meet clinical needs; One is to cultivate cells through a hollow fiber reactor, and one of the culture devices can be found in figure 1 , the main circulation pump 1, the gas exchange tube 2, the culture tube 3 and the culture solution main reservoir 4 connected in sequence through the guide tube, the culture solution main reservoir 4 is provided with a vent hole communicating with the outside world; wherein, the The culture tube 3 is a hollow fiber reactor, and the guide tube includes a first guide tube 11 connecting the main circulation pump 1 and the gas exchange tube 2, connecting the gas exchange tube 2 and the culture tube 3 The second diversion tube 12 of the second guide tube 12, the third guide tube 13 connecting the culture tube 3 and the main culture fluid reservoir 4, and the fourth diversion tube connecting the culture fluid main reservoir 4 and the main circulation pump 1 pipe 14, the third guide pipe 13 communicates with the waste liquid storage tank 5 through the fifth guide pipe 15, wherein the hollow fiber reactor 3 includes a hollow fiber tube and a cylindrical tube for installing the hollow fiber tube The shell, the inside and outside of the hollow fiber tube respectively form a culture solution channel 31 and a cell growth cavity 32, the culture solution channel 31 flows through the culture solution, the cell growth cavity 32 is seeded with cells, and the cells The growth cavity 32 communicates with the fifth flow guide tube through the sixth flow guide tube 16, and the culture solution can enter the cell growth cavity 32 through the aperture of the hollow fiber tube to provide nutrients for the cells to cultivate the cells, The waste liquid produced by the cell culture can be discharged into the waste liquid storage tank 5 through the sixth diversion pipe 16. During this process, the cell culture system is a closed system, and it is difficult to deal with the cell growth process in the closed system in the prior art. Proper adjustment of the dissolved amount of gas (such as oxygen, carbon dioxide, etc.) in the medium makes it difficult for cells to grow under suitable and stable conditions. Therefore, the regulation of the cell growth environment restricts the application of hollow fiber reactors in cell culture

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