Method of quickly identifying tobacco black shank resistance via artificial disease nursery

Abstract

The invention discloses a method of quickly identifying tobacco black shank resistance via an artificial disease nursery. The method comprises steps of manufacturing a black shank inoculants bacteria valley, forming an artificial disease nursery via a layer-cake type inoculating coverage method, maintaining moisture and inducing via an intermittent way and elevating resistance. A feature of susceptibility to diseases of tobacco at a plant field crop land resettling stage and a vigorous-growing period, and biological features of tobacco black shank bacterium surviving, growing and propagating the 5cm depth surface soil are utilized, and the periphery of tobacco plant root parts are inoculated, and a layered-cake type inoculating and covering method of covering a thin layer of organic substance and a thin layer of particular soil to form the artificial disease nursery; and artificial simulation of morning-night intermittent moisture-remaining facilitates shower weather when the black shank disease often happen, and meets high-moisture and ventilating environment requirement for disease occurrence, so the disease can be effectively induced and accuracy and repeatability of tobacco black shank resistance identification can be improved. The method of quickly identifying tobacco black shank resistance via the artificial disease nursery is advantaged by simple operation, water and labor work use reduction and accurate tobacco black shank resistance phenotype identification, thereby being worthy of promotion.

Description

technical field [0001] The invention belongs to the technical field of tobacco disease resistance breeding, and in particular relates to a method for rapidly identifying tobacco black shank resistance in an artificial disease garden. Background technique [0002] The disease garden identification method is a disease resistance identification method formed in the tobacco field growth environment. There are two methods: artificial inoculation and natural induction. It is a traditional method for identification of tobacco black shank disease resistance. The germplasm resistant to black shank in tobacco has been identified and screened by this method for many years and many places, which can reflect the actual resistance level of the variety, but it is easily affected by meteorological conditions and soil bacterial content, and fluctuates greatly between years. Repeated identifications over many years are required. The controllability of its onset conditions seriously restricts...

AI Technical Summary

Problems solved by technology

The national standard GB / T 23224 identification of disease resistance of tobacco varieties regulates the establishment of artificial disease gardens from the aspects of the production of black shank inoculum, inoculation period, and manual quantitative inoculation of bacteria. Although it also mentions the link of irrigation and moisturizing, However, due to the lack of some technical operation details, human factors and environmental conditions have a relatively large impact on the implementation process, and there are still large fluctuations in the identification results.

Images