Asial type foot and mouth disease virus antibody solid-phase competition ELISA kit based on specific monoclonal antibody

Abstract

The invention discloses an Asial type foot and mouth disease virus antibody solid-phase competition ELISA kit based on a specific monoclonal antibody. The Asial type foot and mouth disease virus antibody solid-phase competition ELISA kit contains an HRP labeled Asial type foot and mouth disease specific monoclonal antibody. The kit can be used for monitoring the level of an Asial type foot and mouth disease virus antibody. The kit replaces foot and mouth disease rabbit-anti, guinea antiserum and anti-guinea enzyme labelled antibodies in ELISA, steps of the ELISA method are simplified, stability is improved, detection time is shorted greatly from 24 hours to be within 1.5 hours, and reaction processes requiring primary antibodies, second antibodies and the like in the original ELISA are broken through.

Description

Technical field [0001] The invention relates to the field of biological kits, in particular to a type-specific monoclonal antibody-based Asia1 foot-and-mouth disease virus antibody solid phase competition ELISA kit. Background technique [0002] Foot-and-mouth disease is an acute, febrile, and contact infectious disease caused by cloven-hoofed animals caused by the foot-and-mouth disease virus. It will cause huge economic losses to animal husbandry production and import and export trade. The disease not only seriously affects the development of animal husbandry, but also Human health poses a great hazard. As a large country in cattle and sheep breeding, foot-and-mouth disease is an extremely important disease that harms cattle and sheep breeding. Strengthening the research and development of new technologies for the diagnosis and detection of foot-and-mouth disease, and breaking through the key technologies and processes of the industrialization of foot-and-mouth disease diagnost...

AI Technical Summary

Problems solved by technology

However, with the development of biotechnology, existing methods have shown one or another deficiency, or are cumbersome and time-consuming, or have not reached a very precise level

At present, virus isolation, ELISA, PCR, agglutination test, immunocolloidal gold chromatography, etc. are used in the diagnosis of foot-and-mouth disease. The most widely used is liquid phase blocking ELISA. It takes one day to get the results, so it is impossible to realize the instant detection of a large number of samples in case of an outbreak

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