Solid-phase competition ELISA reagent kit of antibody for O-typed foot and mouth disease virus based on specific monoclonal antibody

Abstract

The invention discloses a solid-phase competition ELISA reagent kit of an antibody for an O-typed foot and mouth disease virus based on a specific monoclonal antibody, which comprises an O-typed foot and mouth disease specific monoclonal antibody marked by HRP (hypothalamic regulatory peptides). The reagent kit of the invention can be used for monitoring a level of the antibody for the O-typed foot and mouth disease virus. By replacing a rabbit antibody, guinea pig antiserum and guinea pig enzyme mark antibody in existing ELISA, the invention simplifies the steps of the ELISA method, improves stability, and greatly shorten detection time from 24 h to 1.5 h or less, thereby breaking through reaction processes for a primary antibody and a second antibody required by original ELISA.

Description

technical field [0001] The invention relates to the field of biological kits, in particular to a type O foot-and-mouth disease virus antibody solid-phase competition ELISA kit based on a type-specific monoclonal antibody. Background technique [0002] Foot-and-mouth disease is an acute, febrile and contagious infectious disease common to cloven-hoofed animals caused by foot-and-mouth disease virus, which will cause huge economic losses to animal husbandry production and import and export trade. Human health poses a great risk. As a large cattle and sheep breeding country in China, foot-and-mouth disease is an extremely important disease that endangers cattle and sheep breeding. Strengthening the research and development of new technologies for the diagnosis and detection of foot-and-mouth disease and breaking through the key technologies and processes for the industrialization of foot-and-mouth disease diagnostic reagents is a solution to the rapid development of animal husb...

AI Technical Summary

Problems solved by technology

However, with the development of biotechnology, existing methods have shown one or another deficiency, or are cumbersome and time-consuming, or have not reached a very precise level

At present, virus isolation, ELISA, PCR, agglutination test, immunocolloidal gold chromatography, etc. are used in the diagnosis of foot-and-mouth disease. The most widely used is liquid phase blocking ELISA. It takes one day to get the results, so it is impossible to realize the instant detection of a large number of samples in case of an outbreak

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