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Refrigeration loading rod

A technology of carrying rods and slides, applied in the field of medical devices, can solve the problems of uneven surface tension, increased thawing time, easy falling of slides, etc., achieves strong hygroscopicity and acid and alkali resistance, avoids cross-infection, good The effect of saving the environment

Active Publication Date: 2017-03-15
BEIJING ZHONGYI KANGWEI MEDICAL INSTR
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0004] The above-mentioned method of carrying embryos has the following defects: first, the slides are directly glued to the glass rod with glue, and the slides are easy to fall during storage; second, the original carrier is the latest plastic carrier. The surface tension is not uniform, and the embryo will appear spherical or flattened during use, which is not conducive to observation and is not conducive to absorbing the freezing liquid around the embryo. If it is directly frozen in this way, the thawing time will be increased during thawing, which will affect the embryo. quality; thirdly, there is no fixed place to place the embryo on the longer carrier, but placed on a certain position on the slide according to the doctor's habit. When observing, it is necessary to find the position of the embryo. The sheath will have the risk of knocking down the embryos; fourth, several people's embryos will be placed in a large liquid nitrogen bucket, and the liquid nitrogen is not used for one-time use, and there will be a risk of virus cross-infection

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0030] The radian rad of the slide 3 is 2.12°, and the thickness d is 0.2mm. When the embryo needs to be frozen, the embryo droplet is placed on the arc slide 3, and then the handle part 1 is removed from the slide 3. Put it outside into the cylindrical hollow tube 4 of the outer casing of the loading rod, and then place the frozen loading rod in the liquid nitrogen tank. Under the action of the steel ring 5, the frozen loading rod will sink rapidly in the liquid nitrogen. The sinking time is shortened, and the freezing rod loaded with embryos is fully in contact with liquid nitrogen for cryopreservation. During the preservation process, the arc-shaped slide 3 of 2.12° can ensure that the frozen droplet is in a three-dimensional shape, ensuring that the frozen droplet can Freezes better.

Embodiment 2

[0032] The radian rad of the slide 3 is 2.32°, and the thickness d is 0.25mm. When the embryo needs to be frozen, the embryo droplet is placed on the arc slide 3, and then the handle part 1 is removed from the slide 3. Put it outside into the cylindrical hollow tube 4 of the outer casing of the loading rod, and then place the frozen loading rod in the liquid nitrogen tank. Under the action of the steel ring 5, the frozen loading rod will sink rapidly in the liquid nitrogen. Shorten its sinking time, let the cryopreservation rod loaded with embryos fully contact with liquid nitrogen for cryopreservation, during the preservation process, the arc-shaped slide 3 of 2.32° can fit the droplet under the natural weight condition The surface curvature maintains the three-dimensional shape of the frozen droplet, allowing the frozen liquid in the frozen droplet to be evenly distributed around the sample, ensuring that the frozen droplet is evenly frozen.

Embodiment 3

[0034]The radian rad of the slide 3 is 2.52°, and the thickness d is 0.3mm. When the embryo needs to be frozen, the embryo droplet is placed on the arc slide 3, and then the handle part 1 is removed from the slide 3. Put it outside into the cylindrical hollow tube 4 of the outer casing of the loading rod, and then place the frozen loading rod in the liquid nitrogen tank. Under the action of the steel ring 5, the frozen loading rod will sink rapidly in the liquid nitrogen. The sinking time is shortened, and the cryopreservation rod loaded with embryos is fully in contact with liquid nitrogen for cryopreservation. During the preservation process, the arc-shaped slide 3 of 2.52° can keep the droplet in a three-dimensional shape under natural weight.

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Abstract

The invention relates to a refrigeration loading rod, and belongs to the technical field of medical instruments. The refrigeration loading rod comprises a loading rod part and a loading rod outer cannula. The loading rod part disclosed by the invention is integrally formed, so as to prevent a slide glass from dropping out; the radian of the designed arc-shaped slide glass can fit for the radian of the surface of a drop under the natural gravity, so that the three-dimensional shape of the refrigerating drop is kept, and refrigerating fluid around a sample in the refrigerating drop can be uniformly distributed, so as to ensure that the refrigerating fluid is uniformly refrigerated; after the slide glass loaded with embryos is loaded in a cylindrical hollow tube, the refrigeration loading rod can quickly sink in liquid nitrogen under the action of a steel ring, so that the refrigeration loading rod can be in full contact with the liquid nitrogen; since the part of the slide glass, which is loaded with the embryos, is completely located in the cylindrical hollow tube, and a cotton plug for fixing a powder plug is formed by weaving synthetic fibers in specific proportion, the air permeability of the embryos is ensured, the moisture absorption and the resistance to acid and base are stronger, a good storage environment is provided for the embryos, and the cross infection of the embryos in a liquid nitrogen pot is effectively avoided.

Description

technical field [0001] The invention belongs to the technical field of medical devices, and in particular relates to a freezing rod. Background technique [0002] With the increase of infertile population, more and more people accept embryo transfer. In open vitrification, embryos are in direct contact with liquid nitrogen, so that the toxic substances in liquid nitrogen may cause potential danger to embryos, so many External fertilization-embryo transfer (IVF) laboratories use closed vitrification as the main method for storing embryos, eggs, blastocysts, etc. [0003] However, the current carrier for carrying embryos is relatively simple and rough. For example, the slide carrying embryos is directly glued to the glass rod with glue, or the glass rod is melted and pulled into a thin sheet, and the embryo is placed on it; or it is carried by a plastic tube. Embryos, the latest product is to put the embryos on the plastic sheet, and then cover the cap, or directly inject a t...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A01N1/02
CPCA01N1/0242
Inventor 费嘉冯涛
Owner BEIJING ZHONGYI KANGWEI MEDICAL INSTR
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