Application of casein kinase PPK related to growth and development of plants

A technology of casein kinase and plant growth, which is applied in the fields of application, plant products, plant gene improvement, etc., and can solve the problems of less functional research

Inactive Publication Date: 2017-03-22
FUJIAN AGRI & FORESTRY UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

However, the function in plants is still less studied. Liu et al. (2003) found that rice casein kinase 1 plays a role in root development and hormone response (Liu et al. 2003)

Method used

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  • Application of casein kinase PPK related to growth and development of plants
  • Application of casein kinase PPK related to growth and development of plants
  • Application of casein kinase PPK related to growth and development of plants

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0032] First, according to PPK1 Sequence Design PCR amplification primer pairs, as shown in Table 1. Using the total cDNA of wild Col-0 Arabidopsis as a template, PCR was obtained PPK1 full sequence. Connect the PCR fragment into the plant expression vector pFGFP, the map is as follows figure 1 . After sequencing, the sequence identical to that of the target gene was obtained. Then, using the Agrobacterium-mediated method, the Arabidopsis rdr6-11 ( Peragine et al. 2004) Plants were infested by dipping flowers. The seeds of the transformed plants were collected and screened for herbicide resistance (Basta), and then transgene-positive plants were obtained through western blot verification, and finally three expressing transgenic lines were obtained. Using wild-type and PPK transgenic Arabidopsis as materials, they were light-treated (blue light: 30 μmol m-2s-1, red light: 30, far-red light: 2.5, dark), and the hypocotyls were measured after growing for 5 days. The m...

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Abstract

The invention provides an application of a casein kinase PPK related to the growth and development of plants. The casein kinase PPK is PPK1, PPK2, PPK3 or PPK4, and the sequence registration numbers thereof are At3G13670, At5G18190, At3G03940 and At2G25760 respectively. The casein kinase PPK is used for improving the growth and development of the plants, and then is applied to crop production.

Description

technical field [0001] The invention belongs to the field of biotechnology, and in particular relates to the application of a kind of casein kinase PPK related to plant growth and development. Background technique [0002] Protein kinases are a class of phosphorylated proteins that use GTP or ATP as phosphate donors to transfer γ-phosphate groups to serine, threonine or tyrosine, thereby regulating related signaling pathways. Eukaryotic protein kinases are a very large superfamily of homologous proteins, including the A-C-G group, the CaMK group, the C-M-G-C group, the conventional protein-casein kinase group, and other protein kinase families (Hanks and Hunter 1995). [0003] In the middle of the 19th century, researchers discovered for the first time a protein kinase capable of phosphorylating casein in rat liver, named casein kinase (Burnett and Kennedy 1954). Since then, casein kinase has attracted widespread attention. At present, compared with the research on casein ...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12N9/12C12N15/54C12N15/82A01H5/00
CPCC12N9/12C12N15/8205C12N15/8261C12N15/827C12Y207/11001
Inventor 柳青蔡大伟黄颖王雪君陈芷馨高红孙世城
Owner FUJIAN AGRI & FORESTRY UNIV
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